A5081717331- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Virology and Viral Diseases
- Animal Disease Management and Epidemiology
- Wildlife Ecology and Conservation
- Rabies epidemiology and control
- Zoonotic diseases and public health
- Bat Biology and Ecology Studies
- Poxvirus research and outbreaks
- Primate Behavior and Ecology
- Insect-Plant Interactions and Control
- Phytoplasmas and Hemiptera pathogens
- Herpesvirus Infections and Treatments
- Respiratory viral infections research
- Plant Pathogens and Fungal Diseases
- interferon and immune responses
- COVID-19 Clinical Research Studies
- Fungal Biology and Applications
- Long-Term Effects of COVID-19
- Insect symbiosis and bacterial influences
- Mycorrhizal Fungi and Plant Interactions
Cornell University
2023-2025
New York State College of Veterinary Medicine
2023-2024
Département Santé Animale
2024
Universidad De Santander
2019-2022
Universidad Antonio Nariño
2022
Leibniz Institute for Zoo and Wildlife Research
2015-2021
Humboldt-Universität zu Berlin
2021
Was the 1993/1994 fatal canine distemper virus (CDV) epidemic in lions and spotted hyaenas Serengeti ecosystem caused by recent spillover of a virulent domestic dog strain or one well adapted to these noncanids? We examine this question using sequence data from 13 'Serengeti' strains including five complete genomes obtained between 1993 2011. Phylogenetic haplotype network analyses reveal that noncanids during were more closely related each other than those wild canids. All noncanid encoded:...
The extent to which the fitness costs of infection are mediated by key life-history traits such as age or social status is still unclear. Within populations, individual heterogeneity in outcome result two successive processes; degree contact with pathogen (exposure) and immune response infection. In mammals, because individuals holding high typically interact more frequently group members, they should be often infected than those low status. However, when access resources determined status,...
Pathology studies of SARS-CoV-2 Omicron variants concern (VOC) are challenged by the lack pathogenic animal models. While BA.1 and BA.2 replicate in K18-hACE2 transgenic mice, they cause minimal to negligible morbidity mortality, less is known about more recent VOC. Here, we show that contrast BA.1, BA.5-infected mice exhibited high levels correlating with higher early viral loads. Neither nor BA.5 replicated brains, unlike most prior Only BA.5–infected substantial weight loss, pathology...
ABSTRACT Feline coronavirus (FCoV) infection normally causes mild or subclinical signs and is common in domestic cats. However, some cats, FCoV can also lead to the development of feline infectious peritonitis (FIP)—a typically lethal disease. has two serotypes genotypes, FCoV-1 FCoV-2, both which cause FIP. The main difference between genotypes viral spike (S) protein that determines tropism pathogenicity, crucial mechanisms Subclinical FIP have been reported wild felids, including...
Abstract Feline coronaviruses (FCoVs) commonly cause mild enteric infections in felines worldwide (termed feline coronavirus [FECV]), with around 12 per cent developing into deadly infectious peritonitis (FIP; virus [FIPV]). Genomic differences between FECV and FIPV have been reported, yet the putative genotypic basis of highly pathogenic phenotype remains unclear. Here, we used state-of-the-art molecular evolutionary genetic statistical techniques to identify compare natural selection...
Background Feline coronavirus infection causes feline infectious peritonitis in a subset of cats, but can also result persistent infection. The tissue reservoirs and the role viral persistence pathogenesis are poorly understood. Aims This study aimed to identify sites naturally infected cat, disease correlates characterise within-host evolution. Methods followed 5-year-old Bengal cat for 6 years collected non-invasive samples, including faeces conjunctival, oropharyngeal saliva swabs. At...
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19), triggered a global pandemic with profound social and economic consequences. The viral spike (S) protein has been identified as key determinant SARS-CoV-2 pathogenicity. In this study, we demonstrate that Omicron BA.4 BA.5 variants, which have closely related S proteins, exhibit different virulence in K18-hACE2 transgenic mice. A comparison genomic sequences revealed...
A 2-year-old male neutered domestic shorthair cat was presented with a progressive history of tetraparesis, ataxia, and inappetence over 4 days. physical exam revealed mucopurulent nasal discharge stertor. neurologic multifocal neurolocalization. The non-ambulatory tetraparetic developed seizures while in hospital. Hematologic assessment anemia, hypoalbuminemia hyperglobulinemia. Magnetic resonance imaging (MRI) the brain meningeal contrast enhancement brainstem cervical spine, as well...
The genus Sapovirus, in the family Caliciviridae, includes enteric viruses of humans and domestic animals. Information on sapovirus infection wildlife is limited currently lacking for any free-ranging species Africa. By screening a large number predominantly fecal samples (n = 631) obtained from five carnivore Serengeti ecosystem, East Africa, RNA was detected spotted hyena (Crocuta crocuta, Hyaenidae), African lion (Panthera leo, Felidae), bat-eared fox (Otocyon megalotis, Canidae), but not...
The Alphacoronavirus-1 species include viruses that infect numerous mammalian species. To better understand the wide host range of these viruses, knowledge on molecular determinants virus-host cell entry mechanisms in wildlife hosts is essential. We investigated infection carnivores using long-term data Serengeti spotted hyenas (Crocuta crocuta) and analyses guided by tertiary structure viral spike (S) attachment protein's interface with receptor aminopeptidase N (APN). sequenced complete...
Feline coronavirus type 1 (FCoV-1 is an alphacoronavirus (species _alphacoronavirus-1_) present in a distinct genetic clade. The FCoV-1 spike protein contains identifiable furin cleavage site (FCS), which highly unusual for alphacoronavirus. widespread and transmissible virus of both domestic non-domestic felids. Notably, following infection with low-pathogenicity virus, pathogenic variants are selected individual cats by process ‘internal mutation’; these appear to have robust tropism...
Abstract Coronaviruses are endemic and can cause disease in a wide range of domestic animal wildlife species. The virus species Alphacoronavirus-1 comprises set diverse viruses that highly recombinogenic, including feline coronavirus type 2 (FCoV-2), which is recombinant genotype 1 (FCoV-1) canine (CCoV-2). Co-infection within host promotes viral recombination; thus, to understand the origin novel variants, it crucial identify hosts be infected with multiple alphacoronaviruses. receptor for...
Feline coronavirus type 1 (FCoV-1 is an alphacoronavirus (species _alphacoronavirus-1_) present in a distinct genetic clade. The FCoV-1 spike protein contains identifiable furin cleavage site (FCS), which highly unusual for alphacoronavirus. widespread and transmissible virus of both domestic non-domestic felids. Notably, following infection with low-pathogenicity virus, pathogenic variants are selected individual cats by process ‘internal mutation’; these appear to have robust tropism...
Feline Coronaviruses (FCoVs) commonly cause mild enteric infections in felines worldwide (termed Enteric Coronavirus [FECV]), with around 12% developing into deadly Infectious Peritonitis (FIP; Virus [FIPV]). Genomic differences between FECV and FIPV have been reported, yet the putative genotypic basis of highly pathogenic phenotype remains unclear. Here, we used state-of-the-art molecular evolutionary genetic statistical techniques to identify compare natural selection pressure sequences,...
Abstract Feline coronavirus type 1 (FCoV-1) is widely known for causing feline infectious peritonitis (FIP), a systemic infection that often fatal, with the virus as FIPV biotype. However, subclinical disease also occurs, in which cats may not show signs and intermittently shed virus, including feces, possibly long periods of time. This FECV Progression to has been linked several genomic changes, however specific region viral spike protein at interface S1 S2 domains especially implicated. In...
Abstract Diverse mammalian species display susceptibility to SARS-CoV-2. Potential SARS-CoV-2 spillback into rodents is understudied despite their host role for numerous zoonoses and human proximity. We assessed exposure infection among white-footed mice ( Peromyscus leucopus ) in Connecticut, USA. observed 1% (6/540) wild-type neutralizing antibody seroprevalence 2020–2022 residential with no cross-neutralization of variants. detected infections via RT-qPCR, but identified non-SARS-CoV-2...
Several coronaviruses (CoVs) have been detected in domesticated, farmed, and wild meso-carnivores, causing a wide range of diseases infecting diverse species, highlighting their important but understudied role the epidemiology these viruses. Assessing viral diversity hosted wildlife species is essential to understand significance cross-species transmission CoVs. Our focus here was on CoV discovery meso-carnivores Northeast United States as potential "hotspot" area with high density humans...
Abstract Small to mid-sized carnivores, or meso-carnivores, comprise a group of diverse mammals, many which can adapt anthropogenically disturbed environments. Wild meso-carnivores living in urban areas may get exposed spread pathogens other species, including stray/feral domestic animals. Several coronaviruses (CoVs) have been detected domesticated and farmed but knowledge CoVs circulating free-ranging wild remains limited. In this study, we analyzed 321 samples collected between 2016 2022...
Alphacoronaviruses are widespread but understudied in comparison to betacoronaviruses. Recombination, mutations, and indels hallmarks of coronavirus replication, which together facilitate viral spillover emergence, especially within the alphacoronaviruses. The species _Alphacoronavirus-1_ comprises distinct viruses cats, dogs pigs. In high-pathogenicity feline (FCoV) is infamous as cause infectious peritonitis (FIP), a lethal disease that can now be treated with antiviral drugs. FCoV-1...
Feline coronavirus (FCoV) infection normally causes mild or subclinical signs and is common in domestic cats. However, some cats, FCoV can also lead to the development of feline infectious peritonitis (FIP), a typically lethal disease. has two serotypes genotypes, FCoV-1 FCoV-2, both which cause FIP. The main difference between genotypes viral spike (S) protein that determines tropism pathogenicity, crucial mechanisms Subclinical FIP have been reported wild felids, including threatened...