A5038384540- Blood transfusion and management
- Immune cells in cancer
- Blood donation and transfusion practices
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Angiogenesis and VEGF in Cancer
- Blood groups and transfusion
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Cell Adhesion Molecules Research
- Trauma and Emergency Care Studies
- Electrospun Nanofibers in Biomedical Applications
- Neonatal Respiratory Health Research
- COVID-19 Clinical Research Studies
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- Hemoglobinopathies and Related Disorders
- Wnt/β-catenin signaling in development and cancer
- Organ Donation and Transplantation
- Immune Response and Inflammation
- Erythrocyte Function and Pathophysiology
- Protease and Inhibitor Mechanisms
- Chemokine receptors and signaling
- Cardiac Fibrosis and Remodeling
Vanderbilt University Medical Center
2015-2024
American Red Cross
2004-2024
Vanderbilt University
2010-2024
World Health Organization Regional Office for South-East Asia
2021
Vanderbilt Health
2021
Canadian Bio-Systems (Canada)
2021
All India Institute of Medical Sciences
2021
Wuhan Blood Center
2021
Shaanxi Blood Center
2021
Tan Tock Seng Hospital
2021
Some observational studies have reported that transfusion of red-cell units been stored for more than 2 to 3 weeks is associated with serious, even fatal, adverse events. Patients undergoing cardiac surgery may be especially vulnerable the effects transfusion.We conducted a randomized trial at multiple sites from 2010 2014. Participants 12 years age or older who were complex and likely undergo red cells randomly assigned receive leukocyte-reduced 10 days less (shorter-term storage group) 21...
Background: The importance of early and aggressive management trauma- related coagulopathy remains poorly understood. We hypothesized that a trauma exsanguination protocol (TEP) systematically provides specified numbers types blood components immediately upon initiation resuscitation would improve survival reduce overall product consumption among the most severely injured patients. Methods: recently implemented TEP, which involves immediate continued release products from bank in predefined...
Introduction: Massive transfusion (MT) protocols have been shown to improve survival in severely injured patients. However, others noted that these higher fresh frozen plasma (FFP):red blood cell (RBC) ratios are associated with increased risk of organ failure. The purpose this study was determine whether MT failure and complications. Methods: Our institution’s exsanguination protocol (TEP) involves the immediate delivery products a 3:2 ratio RBC:FFP 5:1 for RBC:platelets. All patients...
Despite recent attention and impressive results with damage control resuscitation, the appropriate ratio of blood products to be transfused has yet defined. The purpose this study was evaluate whether suggested product ratios yield superior survival rates.After IRB approval, a retrospective evaluation performed on all trauma exsanguination protocol (TEP, n = 118) activations from February 1, 2006 July 31, 2007. A comparison cohort (pre-TEP, 140) selected admissions between August 2004...
There are growing data to suggest that tissue hypoxia represents a critical force drives adult vasculogenesis. Vascular endothelial growth factor (VEGF) expression is dramatically up-regulated by and results in enhanced neovascularization. Although the role of VEGF angiogenesis has been well characterized, its vasculogenesis remains poorly understood. We used two distinct murine bone marrow transplantation (BMT) models demonstrate increased levels at site tumor promoted vivo. This effect was...
Background: Several studies have described predictive models to identify trauma patients who require massive transfusion (MT). Early identification of lethal exsanguination may improve survival in this patient population. The purpose the current study was validate a simplified score predict MT at multiple Level I centers. Methods: All adult treated three centers from July 2006 June 2007 (1) were transported directly scene, (2) activations, and (3) received any blood transfusions during...
Osteoarthritis (OA) is a degenerative joint disease involving both cartilage and synovium. The canonical Wnt/β-catenin pathway, which activated in OA, emerging as an important regulator of tissue repair fibrosis. This study seeks to examine Wnt pathway effects on synovial fibroblasts articular chondrocytes well the therapeutic inhibition OA severity. Mice underwent destabilization medial meniscus surgery were treated by intra-articular injection with XAV-939, small-molecule inhibitor...
The majority of trauma patients (>90%) do not require any blood product transfusion and their mortality is <1%. However, 3% to 5% civilian will receive a massive (MT), defined as >10 units packed red cells (PRBC) in 24 hours. In addition, more than 25% these arrive emergency departments with evidence trauma-associated coagulopathy. With this combination loss coagulopathy, it has become increasingly common transfuse early the PRBC, plasma, platelets. Given inherent uncertainties care severe...
Wnt signaling plays an important role in developmental and stem cell biology. To test the hypothesis that temporary inhibition of will enhance granulation tissue promote angiogenesis repair, we employed a recently characterized small molecule inhibitor. Pyrvinium is FDA-approved drug identified as inhibitor chemical screen for molecules stabilize β-catenin inhibit Axin degradation. Our subsequent characterization pyrvinium has revealed its critical cellular target pathway Casein Kinase 1α....
Background The majority of reports on transfusion reactions address adult patients. Less is known about the types, incidence, and other clinical details in pediatric populations. Furthermore, to our knowledge, there have been no previous directly comparing these aspects between adults patient populations assess if are differences. Study Design Methods Between period January 1, 2011, February 2013, all reported at V anderbilt U niversity M edical C enter ( VUMC ) were evaluated by medicine...
To address the clinical and regulatory challenges of optimal primary endpoints for bleeding patients by developing consensus-based recommendations outcomes pivotal trials in within 6 categories significant bleeding, (1) traumatic injury, (2) intracranial hemorrhage, (3) cardiac surgery, (4) gastrointestinal (5) inherited disorders, (6) hypoproliferative thrombocytopenia.A standardized outcome evaluating hemostatic products strategies treatment clinically will facilitate conduct,...
The United States (US) Expanded Access Program (EAP) to coronavirus disease 2019 (COVID-19) convalescent plasma was initiated in response the rapid spread of severe acute respiratory syndrome 2 (SARS-CoV-2), causative agent COVID-19. While randomized clinical trials were various stages development and enrollment, there an urgent need for widespread access potential therapeutic agents. objective this study is report on demographic, geographical, chronological characteristics patients EAP, key...
Transplantation of mesenchymal stem cells (MSCs) is a promising therapy for ischemic injury; however, inadequate survival implanted in host tissue substantial impediment the progress cellular therapy. Secreted Frizzled-related protein 2 (sFRP2) has recently been highlighted as key mediator MSC-driven myocardial and wound repair. Notably, sFRP2 mediates significant enhancement MSC engraftment vivo. We hypothesized that improves by modulating self-renewal through increasing cell inhibiting...
Abstract Microenvironmental cues instruct infiltrating tumor-associated myeloid cells to drive malignant progression. A subpopulation of coexpressing endothelial and markers, although rare in peripheral blood, are primarily associated with tumors where they enhance tumor growth angiogenesis. These biphenotypic vascular leukocytes result from the differentiation progenitors, a process regulated by necrosis factor (TNF)α vitro. An vivo increase tumor-derived TNFα expression promoted...
Background: Massive transfusion (MT) protocols improve survival in patients with exsanguinating hemorrhage. Both the increased plasma to red blood cells (RBC) and platelets RBC ratios, "protocolization" of product delivery seem be critical components reduction mortality. The purpose this study was identify incidence impact MT protocol noncompliance intervene provider-related events associated poor compliance outcomes. Methods: A initiated 2006 at a Level I trauma center. All cases activation...
Clinical trials utilizing mesenchymal stem cells (MSCs) for severe vascular diseases have highlighted the need to effectively engraft and promote pro‐angiogenic activity. A functional material accomplishing these two goals is an ideal solution as spatiotemporal batch‐to‐batch variability in classical therapeutic delivery can be minimized, tissue regeneration would begin rapidly at implantation site. Gelatin may serve a promising biomaterial due its excellent biocompatibility,...
Understanding differences in gene expression that increase risk for pulmonary arterial hypertension (PAH) is essential to understanding the molecular basis disease. Previous studies on patient samples were limited by end-stage disease effects or use of nonadherent cells, which are not ideal model vascular cells vivo. These addressed hypothesis pathological processes associated with PAH may be identified via a genetic signature common across multiple cell types. Expression array experiments...
Abstract Fibrosis accompanying wound healing can drive the failure of many different organs. Activated fibroblasts are principal determinants post-injury pathological fibrosis along with physiological repair, making them a difficult therapeutic target. Although activated phenotypically heterogeneous, they not recognized as distinct functional entities. Using mice that express GFP under FSP1 or αSMA promoter, we characterized two non-overlapping fibroblast subtypes from mouse hearts after...
Background and objectives COVID-19 convalescent plasma (CCP) has been used, predominantly in high-income countries (HICs) to treat COVID-19; available data suggest the safety efficacy of use. We sought develop guidance for procurement use CCP, particularly low- middle-income (LMICs) which are lacking. Materials methods A multidisciplinary, geographically representative group individuals with expertise spanning transfusion medicine, infectious diseases haematology was tasked development a...
Recent evidence suggests that bone marrow-derived angioblasts or endothelial progenitor cells circulate in peripheral blood and can incorporate at sites of pathologic neovascularization during the ovarian cycle. However, incorporation into vessels nonischemic tissues adult animals has not been observed. We hypothesized vascular microenvironment differs between newborn animals, donor cell progenitors would engraft rapidly growing normal neonatal period. After nonablative administration marrow...
BACKGROUND: The development of RBC autoanti‐bodies resulting from or associated with allogeneic blood transfusions (i.e., autoimmunization) is not a well‐recognized complication transfusions. STUDY DESIGN AND METHODS: T he presentation, laboratory evaluation, clinical course, and management two patients whose autoimmune hemolytic anemia followed transfusion occurred in association the one more alloantibodies described. A retrospective analysis was performed our blood‐bank records over 1 year...