A5036683625- Plant Pathogens and Fungal Diseases
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Resistance
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Banana Cultivation and Research
- Plant Virus Research Studies
- Plant Disease Management Techniques
- Forest Insect Ecology and Management
- Mycorrhizal Fungi and Plant Interactions
- Phytoplasmas and Hemiptera pathogens
- Smart Agriculture and AI
- Plant and animal studies
- Plant and Fungal Interactions Research
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Insect and Pesticide Research
- Plant Physiology and Cultivation Studies
- Species Distribution and Climate Change
- Entomopathogenic Microorganisms in Pest Control
- Insect-Plant Interactions and Control
- Powdery Mildew Fungal Diseases
- Botany and Geology in Latin America and Caribbean
- Animal and Plant Science Education
- Cell Image Analysis Techniques
University of Florida
2016-2025
Plant (United States)
2018-2025
Florida Museum of Natural History
2013
Plant viruses in the genus Begomovirus, family Geminiviridae often cause substantial crop losses. These have been emerging many locations throughout tropics and subtropics. Like plant viruses, they are not recognized by diagnostic clinics due large part to lack of rapid cost effective assays. An isothermal amplification assay, Recombinase polymerase (RPA), was evaluated for its ability detect three begomoviruses suitability use clinics. Methods DNA extraction separation amplicons from...
Laurel wilt, caused by the fungus Raffaelea lauricola, is an exotic disease that affects members of Lauraceae plant family in southeastern United States. The spreading rapidly native forests and now found commercial avocado groves south Florida, where accurate diagnostic method would improve management. A polymerase chain reaction (PCR) based on amplifying ribosomal small-subunit DNA, with a detection limit 0.0001 ng, was to be suitable for some quantitative PCR applications; however, it not...
Citrus canker, caused by Xanthomonas citri subsp. (Xcc) and X. fuscans aurantifolii (Xfa), is a destructive disease of citrus. It sparsely distributed in the Caribbean, effective biosecurity measures, including accurate pathogen detection systems, are essential to prevent further spread. Current in-field screening methods rely on visual symptom identification serological tools like Agdia’s axonopodis (Xac) ImmunoStrips®. However, they failed detect A w C pathotypes this study. Nucleic acid...
Phytophthora ramorum is an invasive, federally regulated pathogen in the United States, with at least 75 confirmed hosts and over 100 associated hosts. As a movement of ornamental plants infected P. across U.S. resulted surge samples experienced by diagnostic laboratories has underscored need for specific rapid screening assay. This work adapted validated previously published RPA tool commercial application. assay was through inclusivity testing four lineages exclusivity on panel species...
Agricultural biosecurity, economical production, and safe trade require a reliable extensive validated diagnostic assay testing process, which is crucial for plant health management. To support this, the United States Department of Agriculture–National Institute Food Agriculture (USDA–NIFA) biosecurity grant program funded creation Diagnostic Assay Validation Network (DAVN) in 2022. This perspective article outlines various activities, tools, resources newly developed DAVN has built since...
Soybean rust (SBR) survival and host availability (kudzu, Pueraria spp.) were assessed from November 2006 through April 2007 at six sites the panhandle to southwest Florida. Micro loggers recorded both temperature relative humidity hourly each location. Periods of drought cumulative hours below 0°C correlated with kudzu defoliation. Inoculum potential detached leaves was evaluated in vitro under various levels. Kudzu SBR kept 4°C produced viable urediniospores highest germination all...
Kudzu (Pueraria spp.) is an accessory host for soybean rust (SBR) (caused by Phakopsora pachyrhizi) that widespread throughout the southeastern United States. An expanded survey of kudzu sites was conducted in 2008 to determine proportion natural resistance north-Florida population. Of 139 evaluated, ≈18% were found be free SBR infection, while 23% had reduced sporulation. Ten accessions from north-central Florida characterized their response challenge a single isolate P. pachyrhizi under...
Phakopsora pachyrhizi Syd. & P. Syd., the cause of soybean rust, was first observed in continental United States November 2004 (2). During growing season 2005, confirmed on (Glycine max) and/or kudzu (Pueraria montana) nine states southern States. It is known that has a much larger host range within Fabaceae family. On 29 September 2005 Quincy, FL, 45 entries mostly large-seeded legumes were planted next to soybeans infected with pachyrhizi. Several seeds each entry one hill. Soybean plants...
A population of adult reproductive female brown widow spiders, Latrodectus geometricus Koch (Araneae: Theridiidae) was collected in North Central Florida. Within weeks, the spiders displayed reduced foraging behavior and began to die. white fungal growth observed on deceased individuals others who showed signs decline. Fungal samples were from living as well recently specimens. Samples excised isolated acidified potato dextrose agar. Morphological characterization cross referenced with most...
Early detection of Asian soybean rust (ASR) is essential to help producers minimize the impact this serious disease. DNA from ASR-infected plants was used compare conventional and real-time ASR-specific PCR assays at seven laboratories in United States. Soybean were inoculated with four concentrations spores establish different levels infection within plants. Plant tissue then harvested 7 time points over course 12 days, extracted using two commercially available kits. spore tested for ASR...
In 2012, stem gall samples on Loropetalum chinense were sent to Florida diagnostic labs from Alabama and nurseries. A fluorescent pseudomonad was consistently isolated the galls. The organism originally identified in based 16S rRNA sequencing as Pseudomonas savastanoi, which causes a production-limiting disease of olive. loropetalum strains reference compared using LOPAT, Biolog, fatty acid analysis, multilocus sequence analysis (MLSA), pathogenicity tests. LOPAT tests placed within...
The National Plant Diagnostic Network (NPDN), comprising diagnostic professionals from more than 70 pathology, entomology, and nematology laboratories, safeguards U.S. plant systems through accurate diagnosis effective communications with clients, partners, stakeholders. As a USDA-NIFA extension program built on the land-grant university system, network has dual responsibilities to clientele such as farmers green industry, well state federal regulatory agencies. Following strategic planning...
Asian soybean rust caused by Phakopsora pachyrhizi was found on and kudzu in Florida November of 2004. The initial diagnosis based observations symptoms urediniospores. two species that cause diseases soybean, P. meibomiae, cannot be differentiated with light microscopy. A rapid DNA extraction PCR amplification protocol discriminated between the species. sequence amplified product confirmed this first report Florida. Accepted for publication 9 May 2005. Published 13 June
Field studies to quantify the effects of shade intensity and duration on soybean rust caused by Phakopsora pachyrhizi were carried out in Florida 2006 2007. Soybean plants at V4 stage inoculated with urediniospores 2100, 0000, 0200 h. Inoculated either placed cages that covered cloths different mesh sizes allowing 70, 50, or 20% transmission sunlight not so received 100% sunlight. Plants kept under 20 sampled 12, 18, 36 h after inoculation determine vivo germination percentage germ tubes...
Plant biosecurity depends heavily on early detection of biological pest and disease threats accurate diagnosis causal agents. The information from these activities is disseminated communicated with decision makers to promote effective mitigation. In the United States, most land-grant university, state, private diagnostic expert identifier labs are successfully networked via U.S. Department Agriculture Animal Health Inspection Service, Protection Quarantine (USDA APHIS PPQ;...
Soybean rust caused by Phakopsora pachyrhizi H. Sydow & was first reported in the continental United States during 2004 (2). By 10 November 2005, disease confirmed eight southern states (Florida, Georgia, Alabama, Mississippi, South Carolina, North Louisiana, and Texas). Diagnoses have been based on visual observation of uredinia urediniospores pathogen followed polymerase chain reaction confirmation. On telia were identified leaves kudzu (Pueraria lobata) central Florida. Telia noted as...