A5019526444- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Acute Myeloid Leukemia Research
- Adrenal and Paraganglionic Tumors
- TGF-β signaling in diseases
- Cancer Genomics and Diagnostics
- Blood Coagulation and Thrombosis Mechanisms
- Lymphoma Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- Lung Cancer Treatments and Mutations
- Bone Metabolism and Diseases
- Pituitary Gland Disorders and Treatments
- Chronic Lymphocytic Leukemia Research
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Connective tissue disorders research
- Epigenetics and DNA Methylation
- Eosinophilic Disorders and Syndromes
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
Central University Hospital of Asturias
2012-2025
Instituto de Investigación Sanitaria del Principado de Asturias
2014-2024
Instituto de Investigación Biosanitaria de Granada
2023
Gobierno del Principado de Asturias
2022
Universidad de Oviedo
2002-2022
Consejo Superior de Investigaciones Científicas
2002
Spanish National Centre for Cardiovascular Research
2002
Centro de Investigaciones Biológicas Margarita Salas
2002
Augusta University
2002
Fundación Hospital de Jove
1998
A cDNA coding for a new human matrix metalloproteinase (MMP) has been cloned from library derived breast tumor. The isolated contains an open reading frame polypeptide of 471 amino acids. predicted protein sequence displays extensive similarity to the previously known MMPs and presents all structural features characteristic members this family, including well conserved PRCGXPD motif, involved in latency enzyme zinc-binding domain (HEXGHXXXXXHS). In addition, novel MMP its acid several...
As cancer cells traverse collagen-rich extracellular matrix (ECM) barriers and intravasate, they adopt a fibroblast-like phenotype engage undefined proteolytic cascades that mediate invasive activity. Herein, we find fibroblasts express an indistinguishable pericellular collagenolytic activity allows them to the ECM. Using isolated from gene-targeted mice, metalloproteinase (MMP)–dependent is identified drives invasion independently of plasminogen, gelatinase A/TIMP-2 axis, B, collagenase-3,...
Significance Relapsed acute lymphoblastic leukemia (ALL) is associated with chemotherapy resistance and poor prognosis. This study analyzes the emergence of acquired mutations in relapsed ALL samples, identifying genes implicated disease progression defining process clonal evolution leading to relapse. These analyses revealed that relapse emerges from subclonal populations sharing only part present dominant population found at diagnosis. Moreover, we show pathways RAS are highly prevalent...
Collagenase 3 (MMP-13) is a recently identified member of the matrix metalloproteinase (MMP) gene family that expressed at high levels in diverse human carcinomas and articular cartilage from arthritic patients. In addition to its expression pathological conditions, collagenase has been detected osteoblasts hypertrophic chondrocytes during fetal ossification. this work, we have evaluated possibility Cbfa1 (core binding factor 1), transcription playing major role osteoblastic specific genes,...
Matrix metalloproteinases (MMPs) have been implicated in numerous tissue-remodeling processes. The finding that mice deficient collagenase-2 (MMP-8) are more susceptible to develop skin cancer, prompted us investigate the role of this protease cutaneous wound healing. We observed a significant delay closure MMP8−/− and an altered inflammatory response their wounds, with neutrophil infiltration during first days persistent inflammation at later time points. These changes were accompanied by...
Remodeling of fibrillar collagen in mouse tissues has been widely attributed to the activity collagenase-3 (matrix metalloproteinase-13 (MMP-13)), main collagenase identified this species. This proposal largely based on repeatedly unproductive attempts detect presence murine interstitial (MMP-1), a major many species, including humans. In work, we have performed an extensive screening genomic and cDNA libraries using as probe full-length for human MMP-1. We report identification two novel...
Collagenase-3 (MMP-13) is a matrix metalloproteinase (MMP) originally identified in breast carcinomas which also produced at significant levels during fetal ossification and arthritic processes. In this work, we have found that transforming growth factor β1 (TGF-β1), widely assumed to be inhibitory for MMPs, strongly induces collagenase-3 expression human KMST fibroblasts. contrast, down-regulated the these cells of collagenase-1 (MMP-1), an enzyme highly related terms structure enzymatic...
Identification of genes associated with hereditary cancers facilitates management patients family histories cancer. We performed exome sequencing DNA from 3 individuals a colorectal cancer who met the Amsterdam criteria for risk nonpolyposis These had mismatch repair-proficient tumors and each carried nonsense variant in FANCD2/FANCI-associated nuclease 1 gene (FAN1), which encodes involved inter-strand cross-link repair. sequenced FAN1 176 additional families vitro functional analyses...
Abstract Matrix metalloproteinases (MMPs) are involved in inflammatory reaction, including asthma-related airway inflammation. MMP-8, mainly produced by neutrophils, has recently been reported to be increased the bronchoalveolar lavage fluid (BALF) from asthmatic patients. To evaluate role of MMP-8 asthma, we measured expression lung tissue an OVA-sensitized mouse model asthma and addressed effect deletion on allergen-induced bronchial production was lungs C57BL/6 mice exposed allergens....
Neutrophil collagenase or 2 (MMP-8) is unique among the family of matrix metalloproteinases (MMPs) because its exclusive pattern expression in inflammatory conditions. At present, no evidence occurrence this enzyme tissues other than human has been reported. In work, we have cloned murine homologue 2. The isolated cDNA contains an open reading frame coding for a polypeptide 465 amino acids, which 74% identical to counterpart. mouse exhibits domain structure characteristic several MMPs,...
Abstract Purpose: Gastrointestinal stromal tumors (GIST) are a distinctive group of mesenchymal neoplasms the gastrointestinal tract. The oncogene KIT has central role in pathogenesis GIST, with c-kit receptor tyrosine kinase (KIT) protein expression being gold standard its diagnosis. identification GIST patients become crucial, because inhibitor Imatinib is effective treatment this malignancy. However, small set GISTs remain unrecognized, not always evident. aim study was new markers for...
The PI3K/AKT/mTOR signaling pathway has emerged as one of the most frequently deregulated in head and neck squamous cell carcinomas (HNSCC). Numerous alterations various upstream downstream components have been described; however, their prognostic significance impact on HNSCC patient survival remains to be established. This was addressed using an unbiased cohort 93 consecutive homogeneous surgically treated patients results confirmed 432 patients. Our findings reveal high prevalence S6...
Genetic diagnosis is recommended for all pheochromocytoma and paraganglioma (PPGL) cases, as driver mutations are identified in approximately 80% of the cases. As list related genes expands, genetic becomes more time-consuming, targeted next-generation sequencing (NGS) has emerged a cost-effective tool. This study aimed to optimize NGS PPGL diagnostics. A workflow based on two customized assays was validated 18 main germline frozen tumor DNA, with one them specifically directed toward...
GCDFP(gross-cystic-disease-fluid protein)-24, a progesterone-binding protein present in large amounts cyst fluid from human breast gross cystic disease, was purified one-step procedure by size-exclusion h.p.l.c. Peptide fragments obtained trypsin digestion of the intact were reverse-phase and analysed for their amino acid composition subjected to automated Edman degradation. A search National Biomedical Research Foundation Data Bank revealed that all sequenced tryptic peptides GCDFP-24...