A5074278844- Bacterial Genetics and Biotechnology
- Cell Adhesion Molecules Research
- Endodontics and Root Canal Treatments
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Protein Kinase Regulation and GTPase Signaling
- Escherichia coli research studies
- Dental Radiography and Imaging
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Transgenic Plants and Applications
- Biochemical and Molecular Research
- PI3K/AKT/mTOR signaling in cancer
- Plant Molecular Biology Research
- DNA Repair Mechanisms
- RNA Research and Splicing
- Microbial Metabolites in Food Biotechnology
- Biochemical Acid Research Studies
- Protease and Inhibitor Mechanisms
- Cardiomyopathy and Myosin Studies
- Cancer Immunotherapy and Biomarkers
James Hutton Institute
2012-2023
The Francis Crick Institute
2016-2022
The Honourable Society of Lincoln's Inn
1944-2016
Cancer Research UK
2014
University of Dundee
2012
University of Oxford
2012
University of Bristol
2007-2011
University of Veterinary Medicine Vienna
2011
St Thomas' Hospital
2007-2010
King's College London
2010
Alternative splicing (AS) coupled to nonsense-mediated decay (NMD) is a post-transcriptional mechanism for regulating gene expression. We have used high-resolution AS RT–PCR panel identify endogenous isoforms which increase in abundance when NMD impaired the Arabidopsis factor mutants, upf1-5 and upf3-1 . Of 270 genes (950 transcripts) on panel, 102 transcripts from 97 (32%) were identified as targets. Extrapolating these data around 13% of intron-containing genome are potentially regulated...
Alternative splicing plays crucial roles by influencing the diversity of transcriptome and proteome regulating protein structure/function gene expression. It is widespread in plants, alteration levels factors leads to a wide variety growth developmental phenotypes. The circadian clock complex piece cellular machinery that can regulate physiology behavior anticipate predictable environmental changes on revolving planet. We have performed system-wide analysis alternative components Arabidopsis...
In pancreatic ductal adenocarcinoma (PDAC), differentiation of stellate cells (PSCs) into myofibroblast-like cancer-associated fibroblasts (CAFs) can both promote and suppress tumor progression. Here, we show that the Rho effector protein kinase N2 (PKN2) is critical for PSC myofibroblast differentiation. Loss PKN2 associated with reduced proliferation, contractility, alpha-smooth muscle actin (α-SMA) stress fibers. spheroid co-cultures PDAC cells, loss prevents invasion but,...
Summary Internalization of food‐borne bacteria into edible parts fresh produce plants represents a serious health risk. Therefore, internalization verocytotoxigenic E. coli O157:H7 isolate Sakai was assessed in two species associated with outbreaks, spinach ( Spinacia oleracea ) and lettuce Lactuca sativa compared to the model Nicotiana benthamiana . occurred leaves roots throughout 10 day time‐course. The plant species, tissue type inoculum dose all impacted outcome. A combination low (~10...
In animals, the protein kinase C (PKC) family has expanded into diversely regulated subgroups, including Rho family-responsive PKN kinases. Here, we describe knockouts of all three mouse isoforms and reveal that PKN2 loss results in lethality at embryonic day 10 (E10), with associated cardiovascular morphogenetic defects. The phenotype was not recapitulated by conditional deletion endothelial cells or developing heart. contrast, inducible systemic after E7 provoked collapse mesoderm....
Type 1 fimbriae (T1F) are well characterised cell surface organelles expressed by Escherichia coli and required for adherence to mannosylated host tissue. They satisfy molecular Koch's postulates as a virulence determinant host-adapted role has been reinforced reports that T1F expression is repressed at submammalian temperatures. Analysis of group 136 environmental animal E. isolates express 37°C showed 28% also capable 20°C, in phase variable manner. The heterogeneous proportions varied...
Novel surfaces derivatized with tertiary amine oxides have been prepared and tested for their ability to resist nonspecific protein adsorption. The oxidation of amines supported on triazine units was carried out using mCPBA give a format allowing conjugation biologically active ligands alongside them. Adsorption these compared adsorption set commercial custom oligo-/poly(ethylene glycol) (OEG/PEG) supports by challenging them display library presented bacteriophage λ. new class oxide is...
Most restriction endonucleases bridge two target sites before cleaving DNA: examples include all of the translocating Type I and III systems, many II nucleases acting at their sites. A subset enzymes, IIB recognise bipartite sequences, like sites, but cut specified phosphodiester bonds near IIS enzymes. However, they make double-strand breaks, one either side site, to release recognition sequence on a short DNA fragment; 34 bp long in case archetype, BcgI. It has been suggested that BcgI...
The endonucleases from the Type IIB restriction–modification systems differ all other restriction enzymes. enzymes cleave both DNA strands at specified locations distant their recognition sequences, like IIS nucleases, but they are unique in that do so on sides of site, to liberate site remainder a short duplex. fact these cut specific mark them as II systems, opposed I randomly, terms gene organization and protein assembly, most have more common with than systems. Our current knowledge is...
The four-component polypeptides of the 2-oxoacid dehydrogenase complex from thermophilic archaeon Thermoplasma acidophilum assemble to give an active multienzyme possessing activity with branched-chain 2-oxoacids derived leucine, isoleucine and valine, pyruvate. dihydrolipoyl acyl-transferase (E2) core is composed identical trimer-forming units that into a novel 42-mer structure comprising octahedral icosahedral geometric aspects. From our previously determined this catalytic core,...
The dihydrolipoyl acyl‐transferase (E2) enzyme forms the structural and catalytic core of tripartite 2‐oxoacid dehydrogenase multienzyme complexes central metabolic pathways. Although this family shares a common architecture, their E2 cores form homo‐trimers that, depending on source, further associate into either octahedral (24‐mer) or icosahedral (60‐mer) assemblies, as predicted by principles quasi‐equivalence. In crystal structure from Thermoplasma acidophilum , thermophilic archaeon,...
Arabinose is a major plant aldopentose in the form of arabinans complexed cell wall polysaccharides or glycoproteins (AGP), but comparatively rare as monosaccharide. l -arabinose an important bacterial metabolite, accessed by pectolytic micro-organisms such Pectobacterium atrosepticum via pectin and hemicellulose degrading enzymes. However, not all plant-associated microbes encode cell-wall-degrading enzymes, yet can metabolize -arabinose, raising questions about their use access to glycan...