A5100659422- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Protease and Inhibitor Mechanisms
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Aldose Reductase and Taurine
- Prenatal Substance Exposure Effects
- Intraocular Surgery and Lenses
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Ocular Disorders and Treatments
- Ophthalmology and Eye Disorders
- Blood Coagulation and Thrombosis Mechanisms
- Spectroscopy Techniques in Biomedical and Chemical Research
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Photoreceptor and optogenetics research
- Biochemical effects in animals
- Connexins and lens biology
- Cannabis and Cannabinoid Research
- Corneal surgery and disorders
- Nitric Oxide and Endothelin Effects
- Laser Applications in Dentistry and Medicine
- Ginseng Biological Effects and Applications
- Dental Implant Techniques and Outcomes
University College London
2011-2025
COMSATS University Islamabad
2024
Kuwait University
2023
Institute of Ophthalmology
1984-2019
New Jersey Institute of Technology
2010-2017
University of London
1984-2015
Moorfields Eye Hospital NHS Foundation Trust
2014
National Institute for Health Research
2014
King's College London
1999-2011
St Thomas' Hospital
1996-2010
The in vitro generation of a three-dimensional (3-D) myocardial tissue-like construct employing cells, biomaterials, and biomolecules is promising strategy cardiac tissue regeneration, drug testing, engineering applications. Despite significant progress this field, current models are not yet able to stably maintain functional characteristics cardiomyocytes for long-term culture therapeutic purposes. objective study was fabricate bioactive 3-D chitosan nanofiber scaffolds using an...
To evaluate the potential role of matrix metalloproteinase (MMP) system Bruch's membrane in pathology age-related macular degeneration.Free and bound pools gelatinase activity membrane-choroid preparations were isolated by phosphate-buffered saline (PBS) sodium dodecyl sulfate (SDS) extraction, respectively. Individual MMP species separated gelatin-substrate zymography levels quantified densitometric techniques. Altogether, 13 control (age range, 71-99 years) 6 AMD 71-95 donor eyes used.All...
In this study, a new 3D liver model was developed using biomimetic nanofiber scaffolds and co-culture system consisting of hepatocytes fibroblasts for the maintenance long-term functions. The chitosan were fabricated by electrospinning technique. To enhance cellular adhesion spreading, surfaces coated with fibronectin (FN) adsorption evaluated various cell types. Cellular phenotype, protein expression, liver-specific functions extensively characterized immunofluorescent histochemical...
Photoreceptor maintenance is dependent on effective delivery of nutrients from the choroidal circulation by way acellular Bruch's membrane and retinal pigment epithelium. Aging associated with thickening, increased cross linking fibers, deposition debris culminating in reduced porosity. The present study has investigated effects aging diffusional transport eight amino acids across 19 human donors. Diffusion studies were carried out Ussing chambers, amount time-dependent transfer preparation...
To investigate cellular dynamics and associated matrix metalloproteinase (MMP) release patterns of human retinal pigment epithelium (RPE) cells subsequent to irradiation by nanosecond pulsed laser at energy levels below visual threshold.Following a stabilization period, RPE-Bruch's-choroid explants were irradiated with system (Q-switched, frequency doubled YAG laser, 532 nm), using 400 μm spot size discontinuous distribution total irradiance 240 mJ/cm², returned the incubator for further 14...
Recovery rate of rod photoreceptor sensitivity (S2 gradient) following a bleach is reduced in age-related macular degeneration (AMD) due to diminished delivery retinol across grossly altered Bruch's membrane. Since triterpenoid saponins are known improve transport Bruch's, we have assessed their possible use for reversing the visual deficits AMD. Double-blind, placebo controlled randomised clinical trial. Altogether 11 AMD patients and seven age-matched control subjects were recruited...
To measure the specific hydraulic conductivity (K) of human sclera over a range ages, to assess topographical variation, and provide theoretical estimate potential scleral outflow facility.Human donor (n = 18; mean age 56.7 +/- 25.9 years; 4-89) was clamped in modified Ussing chamber connected water column set at 15.7 mm Hg. Column descent measured 24 hours 20 degrees C with digital micrometer. Scleral thickness glutaraldehyde-fixed specimens by light microscopy, taking 15 measurements per...
purpose. To determine whether uveal effusion syndrome (UES) is caused by altered scleral permeability to water and large molecules. methods. Transscleral movement was measured using surgically removed sclera clamped in a modified Üssing chamber connected column set at intraocular pressure. Sclera also between two hemichambers, transscleral diffusion of FITC-dextrans (4.4–77 kDa) with spectrophotometer. Clinical data were prospectively collected postal questionnaires. results. Ten patients...
In Brief Purpose: To test the hypothesis that uveal effusion syndrome is caused by reduced transscleral albumin permeability. Methods: Surgical scleral specimens were obtained from a 55-year-old patient with nanophthalmic syndrome. Specimens clamped in modified Ussing chamber, and rate of diffusion fluorescein isothiocyanate–albumin was measured over 12 hours, using spectrophotometer predetermined standard curves. The coefficient determined at 20°C, then adjusted to body temperature...
The ageing of Bruch's membrane is associated with progressive reduction in the degradation capacity for ECM turnover mediated by matrix metalloproteinase (MMP) system. In this study, free and bound pools all gelatinase species were quantified to aid assessing likelihood reduced availability pro-MMPs activation membrane.Bruch's from macular locations (10 eyes; donor age range, 21-84 years) was mounted Ussing chambers eluted phosphate-buffered saline release pool MMPs. Free MMPs subjected...
To quantify the permeability coefficient of albumin across human sclera and to assess topographical age-related variation.Equatorial superotemporal scleral tissue from 15 donor eyes (mean age 60 years; range 39-84) was mounted in a modified Ussing chamber. Additional taken anterior posterior regions six eyes, equatorial superonasal, inferotemporal further eyes. Fluorescein isothiocyanate (FITC)-labeled, 0.412 mM, bovine placed one hemichamber facing internal surface, rate transscleral flux...
This paper introduces a benchtop method for patterning mammalian cells-i.e., culturing cells at specific locations-on planar substrates. Compared with standard cell culture techniques, which do not allow the control of what areas monolayer are populated by one type or another, techniques open new routes to biology. Researchers interested in patterning, however, often times hindered limited access photolithographic capabilities. shows how can be patterned easily sub-millimeter precision using...
Purpose: Beneficial expectations of supplement therapies to increase the transport nutrients, vitamins, and antioxidants across Bruch's membrane in AMD, by mass action alone, remain inconclusive. Therefore, potential for targeting pathways themselves improve bidirectional exchange using amphipathic steroidal glycosides (ginsenosides) has been investigated. Methods: choroid preparations were mounted modified Ussing chambers basal levels hydraulic conductivity (23 donors, age range, 12–89...
To characterize the Michaelis-Menten kinetics of taurine transporter (TT) in retinal pigment epithelium (RPE) freshly isolated from human donor eyes. identify rate limiting compartment pathway delivery choroidal blood supply to outer retina composed by Bruch's-choroid (BC) and RPE older age group.In samples (4 melanoma-affected eyes, 14 control eyes; range, 62-93 years), radiochemical techniques were used determine accumulation at various exogenous concentrations. The transport capability...
The structural and functional demise of aging Bruch's membrane is associated with a reduction in the activity matrix metalloproteinase (MMP) degradation system. gelatinase component MMP system consists MMP2 MMP9 two high molecular-weight (HMW1, HMW2) species that are yet to be characterized whose roles process elucidated. purpose this study was determine age-related changes levels expression subunit characterization HMW membrane.Gelatin zymography followed by densitometric scanning used...
Purpose. Abnormal protein deposits including β -amyloid, found in ageing Bruch’s membrane and brain, are susceptible to degradation by matrix metalloproteinases (MMPs). In membrane, these MMPs become less effective due polymerisation aggregation reactions (constituting the MMP Pathway), a situation much advanced age-related macular degeneration (AMD). The likely presence of this Pathway brain with potential compromise -amyloid associated Alzheimer’s disease (AD) has been investigated....