A5002051079- Platelet Disorders and Treatments
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Blood disorders and treatments
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Retinoids in leukemia and cellular processes
- Adipokines, Inflammation, and Metabolic Diseases
- Drug Transport and Resistance Mechanisms
- Metabolism, Diabetes, and Cancer
- Blood properties and coagulation
- IL-33, ST2, and ILC Pathways
- Liver Disease Diagnosis and Treatment
- Psoriasis: Treatment and Pathogenesis
- Antiplatelet Therapy and Cardiovascular Diseases
- Cancer, Lipids, and Metabolism
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Chronic Lymphocytic Leukemia Research
- Mast cells and histamine
- Acute Ischemic Stroke Management
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cytokine Signaling Pathways and Interactions
- Kruppel-like factors research
- Stroke Rehabilitation and Recovery
- Ginger and Zingiberaceae research
Institut Pasteur de Lille
2007-2020
Inserm
2006-2020
Université de Lille
2001-2020
Centre Hospitalier Universitaire de Lille
2016-2020
Centre National de la Recherche Scientifique
2013-2015
Center for Infection and Immunity of Lille
2015
Université Lille Nord de France
2011-2013
The University of Western Australia
2013
European Genomic Institute for Diabetes
2013
Institut National de Recherche en Santé Publique
2012
The liver X receptors (LXRs) are nuclear that form permissive heterodimers with retinoid receptor (RXR) and important regulators of lipid metabolism in the liver. We have recently shown RXR agonist-induced hypertriglyceridemia hepatic steatosis mice dependent on LXRs correlate an LXR-dependent induction lipogenic genes. To further investigate roles LXR regulation gene expression, we mapped ligand-regulated genome-wide binding these factors mouse find agonist bexarotene primarily increases...
In the present study, we have addressed role of linker for activation T cells (LAT) in regulation phospholipase Cγ2 (PLCγ2) by platelet collagen receptor glycoprotein VI (GPVI). LAT is tyrosine phosphorylated human platelets heavily response to collagen, collagen-related peptide (CRP), and FcγRIIA cross-linking but only weakly G-protein-receptor-coupled agonist thrombin. phosphorylation abolished CRP-stimulated Syk-deficient mouse platelets, whereas it not altered SLP-76-deficient mice or...
The adapter protein SLP-76 is expressed in T lymphocytes and hematopoietic cells of the myeloid lineage, known to be a substrate tyrosine kinases that are activated after ligation T-cell antigen receptor. Transient overexpression line potentiates transcriptional activation receptor ligation, while loss expression abrogates several receptor-dependent signaling pathways. Mutant mice lack manifest severe block at an early stage thymocyte development, implicating events promote maturation. While...
The collagen receptor glycoprotein VI (GPVI) induces platelet activation through a similar pathway to that used by immune receptors. In the present study we have investigated role of phosphatidylinositol 3-kinase (PI 3-kinase) in GPVI signalling. Our results show collagen-related peptide {CRP: [GCP*(GPP*)(10)GCP*G](n); P*=hydroxyproline}, which is selective GPVI, formation 3,4,5-trisphosphate [PI(3,4,5)P(3)] and 3,4-bisphosphate [PI(3, 4)P(2)] platelets. increase two 3-phosphorylated lipids...
Collagen-related peptide (CRP), a collagen homologue, induces platelet activation through tyrosine kinase-dependent pathway, leading to sequential phosphorylation of Fc receptor (FcR) γ-chain, Syk, and phospholipase C-γ<sub>2</sub>. Here we report that CRP the low affinity immune FcγRIIA stimulate T cell adapter SLP-76, whereas G protein-coupled agonist thrombin only minor phosphorylation. This suggests SLP-76 has specific role downstream receptors signal via an immunoreceptor tyrosine-based...
Fibrate treatment in mice is known to modulate high density lipoprotein (HDL) metabolism by regulating apolipoprotein (apo)AI and apoAII gene expression. In addition alterations plasma HDL levels, fibrates induce the emergence of large, cholesteryl ester-rich treated transgenic expressing human apoAI (HuAITg). The mechanisms these changes may not be restricted modulation expression, aim present study was determine whether expression factors affect (<i>i.e.</i> phospholipid transfer protein...
Bexarotene (Targretin) is a clinically used antitumoral agent which exerts its action through binding to and activation of the retinoid-X-receptor (RXR). The most frequent side-effect bexarotene administration an increase in plasma triglycerides, independent risk factor cardiovascular disease. molecular mechanism behind this hypertriglyceridemia remains poorly understood.Using wild-type LXR alpha/beta-deficient mice, we show here that induces activates hepatic LXR-target genes lipogenesis...
Obesity is associated with a significantly increased risk for cancer suggesting that adipose tissue dysfunctions might play crucial role therein. Macrophages important roles in as well cancers. Here, we studied whether human macrophages (ATM) modulate cell function. Therefore, ATM were isolated and compared monocyte-derived (MDM) from the same obese patients. ATM, but not MDM, found to secrete factors inducing inflammation lipid accumulation T47D HT-29 cells. Gene expression profile...
Platelet activation by collagen is mediated the sequential tyrosine phosphorylation of Fc receptor γ‐chain (FcR γ‐chain), which part glycoprotein VI, kinase Syk and phospholipase C‐γ2 (PLC‐γ2). In this study tyrosine‐phosphorylated proteins that associate with PLC‐γ2 after stimulation a collagen‐related peptide (CRP) were characterized using glutathione S‐transferase fusion Src homology (SH) domains immunoprecipitation endogenous PLC‐γ2. The majority bind to its C‐terminal SH2 domain. These...
Dyslipidemia contributes to endothelial dysfunction in type 2 diabetes mellitus. Fenofibrate (FF), a ligand of the peroxisome proliferator-activated receptor-α (PPARα), has beneficial effects on microvascular complications. FF may act endothelium by regulating vasoactive factors, including endothelin-1 (ET-1). In vitro, decreases ET-1 expression human cells. We investigated molecular mechanisms involved effect treatment plasma levels mellitus patients.FF impaired capacity transforming growth...
The α-1-acid glycoprotein/orosomucoids (ORMs) are members of the lipocalin protein family. Encoded by 3 polymorphic genes in mouse (2 man, 1 rat), ORMs expressed hepatocytes and function as acute-phase proteins secreted plasma under stressful conditions. In addition to their role nanocarrier, involved several pathophysiological processes such immunosuppression, cardioprotection, inflammatory bowel disease. nuclear bile acid receptor farnesoid X (FXR) regulates homeostasis lipid glucose...
The sex steroid progesterone is essential for the proliferation and differentiation of mammary gland epithelium during pregnancy. In relation to this, in vitro studies using breast carcinoma T47D cells have demonstrated a biphasic response, consisting an initial proliferative burst followed by sustained growth arrest. However, transcriptional factors acting with receptor (PR) mediate effects on cell remain be determined. Recently, it has been that regulating protein 132 kDa (TReP-132),...
<i>Background:</i> We intended to determine ethnic differences in the characteristics, management and outcome of acute ischemic stroke between Israeli Arab Jewish populations. <i>Methods:</i> A national survey was conducted 2004 at all 28 hospitals Israel. Information on demographics, transportation, risk factors, clinical presentation, severity, type subtype, obtained. Mortality during 36 months after hospitalization assessed by matching with mortality data....
Humanized mice for the nuclear receptor peroxisome proliferator-activated δ (PPARδ), termed PPARδ knock-in (PPARδ KI) mice, were generated investigation of functional differences between mouse and human as tools early drug efficacy assessment.Human function in lipid metabolism was assessed at baseline, after fasting or when challenged with GW0742 compound fed a chow diet high-fat (HFD).Analysis mRNA levels revealed hypomorph expression liver, macrophages, small intestine heart, but not...
The occurrence of new blood vessel formation in the lungs asthmatic patients suggests a critical role for airway endothelial cells (ECs) disease. IL-33 (Interleukin-33)-a cytokine abundantly expressed human lung ECs-recently emerged as key factor development allergic diseases, including asthma. In present study, we evaluated whether mouse and ECs exposed to common Dermatophagoides farinae allergen produce characterized activated signaling pathways. Approach Results: Mouse primary were vitro...
Abstract Retinoic acid receptor-related orphan receptor-alpha (RORα) is a transcription factor from the nuclear receptor family expressed by immune cells and involved in development of obesity, insulin resistance (IR) non-alcoholic steatohepatitis (NASH). It was recently reported that mice deficient for RORα macrophages develop more severe NASH upon high fat diet (HFD) feeding due to altered Kupffer cell function. To better understand role obesity IR, we independently generated macrophage...