A5082012795- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Tuberculosis Research and Epidemiology
- Vibrio bacteria research studies
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Food Safety and Hygiene
- Mycobacterium research and diagnosis
- Drug Transport and Resistance Mechanisms
- Mass Spectrometry Techniques and Applications
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Cancer therapeutics and mechanisms
- Streptococcal Infections and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Microbial infections and disease research
- Pneumonia and Respiratory Infections
- Biochemical and Structural Characterization
University of KwaZulu-Natal
2016-2025
University of Venda
2023-2024
University of Limpopo
2024
University of Zululand
2016
Care Resource
2013
The antileprosy drug clofazimine has shown potential for shortening tuberculosis treatment; however, the current dosing of is not evidence based, and optimal unknown. Our objective was to conduct a preclinical evaluation pharmacokinetics pharmacodynamics in mouse model tuberculosis, with goal providing useful information on future studies. Pharmacokinetic parameters were evaluated infected uninfected BALB/c mice. Pharmacodynamic Mycobacterium tuberculosis-infected mice that treated 12 weeks...
Antibiotic resistance profiles of Escherichia coli were investigated in an intensive pig production system the uMgungundlovu District, South Africa, using ‘farm-to-fork’ approach. Four hundred seventeen (417) samples collected from and products at different points (farm, transport, abattoir). E. was isolated enumerated Colilert® 18/Quanti-Tray® 2000 system. Ten isolates each Quanti-tray selected randomly putatively identified on eosin methylene blue agar. Real-time PCR targeting uidA gene...
The anti-leprosy drug clofazimine has been shown to have antimicrobial activity against Mycobacterium tuberculosis and associated with treatment-shortening in both clinical preclinical studies of TB chemotherapy. However, a reported lack early bactericidal (EBA) patients raised questions regarding the usefulness as an anti-TB drug. Our objective was systematically evaluate EBA vitro vivo provide insight into how when this exerts its M. tuberculosis. We evaluated 14 day (i) at concentrations...
The antileprosy drug clofazimine was recently repurposed as part of a newly endorsed short-course regimen for multidrug-resistant tuberculosis. It also enables significant treatment shortening when added to the first-line drug-susceptible tuberculosis in mouse model. However, causes dose- and duration-dependent skin discoloration patients, optimal dosing strategy context is unknown. We utilized well-established model systematically address impacts duration, dose, companion drugs on...
This study aimed at investigating the use of metal chelators as potential metallo‐β‐lactamase inhibitors (MBL). The minimum inhibitory concentration (MIC) meropenem was ascertained alone and in combination with various concentrations macrocyclic (1,4,7‐ triazacyclononane‐1‐glutaric acid‐4,7‐diacetic acid = NODAGA) peptide derivatives acyclic (N,N,N′,N′‐Tetrakis(2‐pyridylmethyl)ethylenediamine TPEN di‐(2‐picolyl)amine= DPA) using broth microdilution method. MICs against carbapenem‐resistant...
Coagulase-negative staphylococci (CoNS) are increasingly associated with nosocomial infections, especially among the immunocompromised and those invasive medical devices, posing a significant concern. We report on clinical multidrug-resistant CoNS from uMgungundlovu District, KwaZulu-Natal Province, South Africa, as emerging pathogens. One hundred thirty presumptive were obtained blood cultures. Culture, biochemical tests, Staphaurex™ Latex Agglutination Test used for initial identification...
Campylobacter spp. are among the leading foodborne pathogens, causing campylobacteriosis, a zoonotic infection that results in bacterial gastroenteritis and diarrheal disease animals humans. This study investigated molecular epidemiology of antibiotic-resistant isolated across farm-to-fork-continuum an intensive pig production system South Africa. Following ethical approval, samples were collected over sixteen weeks from selected critical points (farm, transport, abattoir, retail) using...
Background: This study determined the prevalence and antibiotic susceptibility profiles of Staphylococcus aureus isolated from selected critical control points (farm, transport, abattoir, retail product) in an intensive poultry production system uMgungundlovu District, South Africa, using “farm to fork” approach. Materials Methods: Three hundred eighty-four samples products were examined across continuum for S. selective media, biochemical tests, API Staph kit confirmed by polymerase chain...
Campylobacter jejuni isolated from broiler and layer chickens registered abattoirs in KwaZulu-Natal, South Africa, were tested for their susceptibility to eight antibiotics. Using agar dilution, antibiotics was determined C. recovered the caeca. A total of 155 isolates collected which 77 identified as (broilers n = 56 layers 21). Resistance highest tetracycline 98.2% 100%) ceftriaxone 96.4% 100%). High found ciprofloxacin 91% 76%) gentamicin 98% 81%). Susceptibilities each broilers layers,...
There is a dearth of surveillance data on clinical Campylobacter in South Africa, particularly the private healthcare environment. We investigated prevalence resistance to first-line antibiotics used treat campylobacterioses isolates from pathology laboratory.Identification specific genes were confirmed by PCR. Minimum inhibitory concentrations determined using broth micro-dilution method against macrolides (erythromycin, azithromycin), fluoroquinolones (ciprofloxacin, gatifloxacin) and...
Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococcus (VRE) are included on the WHO high priority list of pathogens that require urgent intervention. Hence emphasis needs to be placed developing novel class molecules tackle these pathogens. Teixobactin is a new antibiotic has demonstrated antimicrobial activity against common bacteria. Here we examined properties three derivatives clinically relevant bacterial isolates taken from South African patients. The...
ABSTRACT Experimental and clinical studies have indicated that the antileprosy drug clofazimine may contribute treatment-shortening activity when included in tuberculosis treatment regimens. Clofazimine accumulates to high levels tissues, has a long half-life, remains body for months after administration is stopped. We hypothesized treatment, accumulated sustained antimicrobial cessation, we used BALB/c mouse model of chronic chemotherapy address this hypothesis. Mycobacterium -infected mice...
This study investigated the antibiotic resistance, virulence profiles, and clonality of Campylobacter jejuni coli isolated from an intensive poultry farming system in KwaZulu-Natal, South Africa. Following ethical approval, samples were collected over six weeks using farm-to-fork approach. spp. identified culture, confirmed differentiated to species level by PCR, subjected susceptibility testing. Selected resistance (and mutations) genes screened PCR DNA sequencing. Genetic relatedness...
The presence of the zoonotic pathogen Salmonella in food supply chain poses a serious public health threat. This study describes prevalence, susceptibility profiles, virulence patterns, and clonality from poultry flock monitored over six weeks, using farm-to-fork approach. was isolated selective media confirmed to genus species level by real-time polymerase reaction (RT-PCR) invA iroB genes, respectively. Antimicrobial profiles were determined Vitek-2 Kirby-Bauer disk diffusion method...