A5000549419- Mitochondrial Function and Pathology
- Redox biology and oxidative stress
- Antibiotic Resistance in Bacteria
- Protein Structure and Dynamics
- Trace Elements in Health
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Microbial Metabolic Engineering and Bioproduction
- Probiotics and Fermented Foods
- Sulfur Compounds in Biology
- Essential Oils and Antimicrobial Activity
- Transgenic Plants and Applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Biochemical effects in animals
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- ATP Synthase and ATPases Research
Universidade de São Paulo
2012-2024
Yeast Prx1 is a mitochondrial 1-Cys peroxiredoxin that catalyzes the reduction of endogenously generated H2O2. synthesized on cytosolic ribosomes as preprotein with cleavable N-terminal presequence targeting signal, but mechanisms underlying distribution to distinct subcompartments are unknown. Here, we provide direct evidence following dual localization Prx1: soluble form in intermembrane space and matrix weakly associated inner membrane. We show sorting into likely involves release protein...
A novel transposon belonging to the Tn3-like family was identified on chromosome of a commensal strain Pseudomonas aeruginosa sequence type 2343 (ET02). Tn6350 is 7,367 bp long and harbors eight open reading frames (ORFs), an ATPase (IS481 family), transposase (DDE catalytic type), Tn3 resolvase, three hypothetical proteins, genes encoding new pyocin S8 with its immunity protein. We show that displays activity against carbapenemase-producing P. aeruginosa, including IMP-1, SPM-1, VIM-1,...
Multidrug resistance (MDR) is a serious threat to public health, making the development of new antimicrobials an urgent necessity. Pyocins are protein antibiotics produced by Pseudomonas aeruginosa strains kill closely related cells during intraspecific competition. Here, we report in-depth biochemical, microbicidal, and structural characterization S-type pyocin, named S8. Initially, described domain organization secondary structure Subsequently, observed that recombinant S8 composed killing...
Abstract Multi-drug resistance (MDR) is a serious threat to global public health, making the development of new antimicrobials an urgent necessity. Pyocins are protein antibiotics produced by Pseudomonas aeruginosa strains kill closely related cells during intraspecific competition. Here, we report in depth biochemical, microbicidal and structural characterization S-type pyocin, named S8. Initially, described domain organization secondary structure Subsequently, observed that recombinant S8...
Despite recent advances in the characterization of yeast mitochondrial proteome, submitochondrial localization a significant number proteins remains elusive. Here, we describe robust and effective method for determining suborganellar proteins, which is considered fundamental step during protein function elucidation. This involves an initial that consists obtaining highly pure intact mitochondria. These preparations are then subjected to subfractionation protocol consisting hypotonic shock...
Despite recent advances in the characterization of yeast mitochondrial proteome, submitochondrial localization a significant number proteins remains elusive. Here, we describe robust and effective method for determining suborganellar proteins, which is considered fundamental step during protein function elucidation. This involves an initial that consists obtaining highly pure intact mitochondria. These preparations are then subjected to subfractionation protocol consisting hypotonic shock...