A5030468719- Redox biology and oxidative stress
- Mitochondrial Function and Pathology
- Antibiotic Resistance in Bacteria
- Coenzyme Q10 studies and effects
- Trace Elements in Health
- ATP Synthase and ATPases Research
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Biochemical effects in animals
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- Biochemical and Molecular Research
- Biochemical Acid Research Studies
- bioluminescence and chemiluminescence research
- Protein Structure and Dynamics
- Microbial Natural Products and Biosynthesis
- Seed Germination and Physiology
- Ferroptosis and cancer prognosis
- Plant nutrient uptake and metabolism
- Plant and animal studies
- Genetics and Neurodevelopmental Disorders
- Selenium in Biological Systems
- Metalloenzymes and iron-sulfur proteins
- Heme Oxygenase-1 and Carbon Monoxide
- Bioenergy crop production and management
Universidade de São Paulo
2016-2025
Marshfield Clinic
2010
Pontifícia Universidade Católica do Rio Grande do Sul
2010
Universidade Federal do Rio Grande do Sul
2010
Fundação Faculdade de Odontologia
2010
Hospital de Clínicas de Porto Alegre
2010
Yeast Prx1 is a mitochondrial 1-Cys peroxiredoxin that catalyzes the reduction of endogenously generated H2O2. synthesized on cytosolic ribosomes as preprotein with cleavable N-terminal presequence targeting signal, but mechanisms underlying distribution to distinct subcompartments are unknown. Here, we provide direct evidence following dual localization Prx1: soluble form in intermembrane space and matrix weakly associated inner membrane. We show sorting into likely involves release protein...
Saccharomyces cerevisiae has three distinct inner mitochondrial membrane NADH dehydrogenases mediating the transfer of electrons from to CoQ (coenzyme Q): Nde1p, Nde2p and Ndi1p. The active site Ndi1p faces matrix side, whereas enzymatic activities Nde1p are restricted intermembrane space where they responsible for cytosolic oxidation. In present study we genetically manipulated yeast strains in order alter redox state evaluate consequences on mtDNA (mitochondrial DNA) maintenance....
Nuclear mitochondrial DNA insertions (NUMTs) are sequences that have been transferred into the nucleus and recognized by presence of indels stop codons. Although NUMTs identified in a diverse range species, their discovery was frequently accidental. Here, our initial goal to develop standardize simple method for isolating from nuclear genome single bee. Subsequently, we tested new protocol determining whether codons cytochrome c oxidase subunit I (COI) sequence Melipona flavolineata origin....
Maria Cristina Munerato, PhD*, Sabrina Pozatti Moure, MSc Buccal Pathology†, Vivian Machado, Resident Doctor‡ and Fernando Grilo Gomes, Psychiatry‡ *Clinical Stomatology, Pontifical Catholic University of Rio Grande do Sul, Brazil †Buccal Pathology, Federal ‡Psychiatry, Hospital de Clínicas Porto Alegre (HCPA), Corresponding Author: Dra. Faculdade Odontologia – UFRGS, Rua Ramiro Barcelos, 2492, Departamento Conservadora (DOC), Alegre, RS. Brazil. 90035-003, Tel: +55 51 33165005, Fax:...
A novel transposon belonging to the Tn3-like family was identified on chromosome of a commensal strain Pseudomonas aeruginosa sequence type 2343 (ET02). Tn6350 is 7,367 bp long and harbors eight open reading frames (ORFs), an ATPase (IS481 family), transposase (DDE catalytic type), Tn3 resolvase, three hypothetical proteins, genes encoding new pyocin S8 with its immunity protein. We show that displays activity against carbapenemase-producing P. aeruginosa, including IMP-1, SPM-1, VIM-1,...
Thiol peroxidases (TP) are ubiquitous and abundant antioxidant proteins of the peroxiredoxin glutathione peroxidase families that can catalytically rapidly reduce biologically relevant peroxides, such as hydrogen peroxide peroxynitrite. However, TP catalytic cycle is complex, depending on multiple redox reactions partners, subjected to branching competition points may limit their reductase activity in vivo. The goals present study were demonstrate peroxynitrite members live cells real time...
Multidrug resistance (MDR) is a serious threat to public health, making the development of new antimicrobials an urgent necessity. Pyocins are protein antibiotics produced by Pseudomonas aeruginosa strains kill closely related cells during intraspecific competition. Here, we report in-depth biochemical, microbicidal, and structural characterization S-type pyocin, named S8. Initially, described domain organization secondary structure Subsequently, observed that recombinant S8 composed killing...
Abstract Based on available platforms of Saccharomyces cerevisiae mitochondrial proteome and other high throughput studies, we identified the yeast gene DMO2 with a profile genetic physical interactions that indicate putative role in respiration. Dmo2p is homolog to human DMAC1 two conserved cysteines Cx 2 C motif. Here, localized inner membrane facing intermembrane space. The observed phenotypes dmo2 null mutant general function cellular stress response; displayed poor growth...