This study was conducted to the effects of ensiled alfalfa (Medicago sativa) and red clover (Trifolium pratense) at different ratios on dynamics fermentation parameters, N distribution, protein fractions, protease activities during ensiling. Alfalfa were harvested wilted 35 25% dry matter, respectively, chopped 1 cm, mixed, weighed into 1.0-L buckets a density 700 g/L, for 1, 3, 7, 15, 30 d 30°C. The treatments mixing ratio 100:0, 70:30, 50:50, 30:70, 0:100 (R0, R30, R50, R70, R100,...

Two lactic acid bacteria (LAB) strains with different ferulic esterase (FAE) activities were isolated: Lactobacillus farciminis (LF18) and plantarum (LP23). The effects of these on the fermentation quality, in vitro digestibility phenolic extraction yields sorghum (Sorghum bicolor L.) silage studied at 20, 30 40 °C. Sorghum was ensiled no additive (control), LF18 or LP23 for 45 days. At °C, content decreased, whereas ammonia nitrogen (NH3-N) significantly increased (p < 0.05). all three...

Inflammatory bowel disease is known to be associated with alterations in gut microbiota. The bioactive compound syringic acid has been shown alleviate inflammatory disease, but its interaction microbiota and mechanism of action remain unclear. To address this, we conducted a study which investigated the potential benefits mouse model dextran sulfate sodium-induced colitis through modulation. Our results show that oral administration effectively reduced symptoms colitis, as indicated by...

This study aimed to evaluate the effect of application an inoculant and a preservative on fermentation quality, in vitro digestibility, aerobic stability alfalfa silage-based fermented total mixed ration (TMR). The TMR was ensiled with (1) no additives (control), (2) Lactobacillus plantarum (LP), or (3) potassium sorbate (PS). V-scores all silages were higher than 80 points during 30 days ensiling. addition LP PS had effects parameters, such as digestibility gas production (p > 0.05)....

This study explored the effects of four sugar source treatments, including no (CON), fructose (FRU), pectin (PEC), and starch (STA), on microbiota metabolome alfalfa (Medicago sativa) silage. The bacterial community was determined via 16S rRNA gene sequencing. analyzed using ultra high-performance liquid chromatography tandem time-of-flight mass spectrometry (UHPLC/TOF-MS). After 60 days fermentation, pH values in FRU PEC were lower than those STA CON. had a greater lactic acid concentration...

This study aimed to evaluate the effects of different proportions alfalfa silage on fermentation quality, in vitro digestibility, and aerobic stability total mixed ration (TMR) silage. Three TMRs were prepared with contents a fresh matter basis: (1) 60% (AS60), (2) 40% (AS40), (3) 20% (AS20). The lactic acid AS60 did not increase after 30 days ensiling (p > 0.05). Butyric was detected AS20 group 14 ensiling. showed significantly higher dry digestibility than < TMR gradually increased...

<title>Abstract</title> Whole corn silage (WCS) is prone to deterioration during storage, but appropriate supplements can enhance active metabolites, preserving quality and feeding value. This study compared the metabolic responses of WCS treated with <italic>Lactobacillus buchneri</italic> (LB), plant-derived melatonin (ME), their combination (LB + ME) by evaluating fermentation quality, antioxidant indices, metabolites. LB ME significantly increased superoxide dismutase catalase activities...

Dairy products are the major source of odd- and branched-chain fatty acids (OBCFAs), a group nutrients with emerging health benefits. The animal diet is known to influence milk fat OBCFAs dairy cows; however, little about effects physiological factors. objective this study was investigate parity lactation stage on in cows. Holstein cows (n = 157) were selected according (first, second, third, or greater) days (DIM) (≤21 DIM, 21 < DIM ≤ 100, 100 200, >200 DIM). All fed same total mixed ration...

Abstract The effects of Lactobacillus plantarum ( LP ) and Chinese gallnut Rhus chinensis Mill) tannin on the fermentation quality, nitrogen distribution, protein fractions proteases activity alfalfa Medicago sativa silage were studied. Additives added to forage (approximately 40% DM (1 × 10 6 cfu/g FW plus sucrose (4 g/kg + S), commercial cellulase (0.1 C) at two levels (20 50 TA 2% 5%). control was sprayed with same volume distilled water. Silage sampled analysed days 1, 3, 5, 7, 14, 21,...

In this study, an experiment was performed to evaluate the effect of lactic acid bacteria and 3-phenyllactic (PLA) on fermentation quality chemical composition alfalfa silage. Several PLA-tolerant strains were screened from silages identified. The selected (1 × 106 colony forming units/g fresh alfalfa) PLA (1.0, 2.0, or 3.0 g/kg) applied before ensiling. After 45 days storage, unsealed subjected component analysis. Biochemical methods 16S rDNA gene sequencing used for identification two as...

Abstract The effect of Lactobacillus plantarum ‘KR107070’ and a propionic acid‐based preservative on the quality aerobic stability alfalfa‐corn mixed silage ensiled with four ratios was evaluated. A 4 × factorial arrangement used in completely randomized design this study. chopped alfalfa corn were made into to (containing 20, 40, 60 80% corn), each which treated (i) distilled water; (ii) 1 10 6 colony forming units per g L. ‘KR107070’; (iii) chemical additive consisting 0.24% acid, sodium...

Abstract This study evaluated the effect of three isolated lactic acid bacteria (LAB) inoculants on ensiling characteristics, aerobic stability and in vitro digestibility low dry matter whole‐crop oat silages (WCOS). The oats were harvested at two stages maturity, boot filling stages, then inoculated with 1 × 10 6 cfu/g fresh material Lactobacillus plantarum (LP), rhamnosus (LR) buchneri (LB). After 45 days ensiling, LAB increased (LA) concentrations ( p &lt; .05) decreased ammonia nitrogen...

Alfalfa sometimes cannot be harvested in time due to the rainy season. To improve fermentation quality, protein quality and digestibility of alfalfa silage late, Lactobacillus plantarum (LP) molasses were applied an actual production process this study. at full bloom stage was ensiled with (1) distilled water (control), (2) 1 × 106 colony-forming units LP/g fresh matter, (3) 15 g molasses/kg matter (M) or (4) LP + M (LPM) for 55 days. and/or showed significantly lower pH ammonia nitrogen...

The objective of this experiment was to evaluate the effects chopping length and additive on fermentation characteristics aerobic stability in silage Leymus chinensis. L. chinensis chopped 1–2 cm 4–5 cm, immediately ensiled with three treatments, i.e., 2% sucrose (fresh weight basis; SU), 1 × 105 cfu/g Lactobacillus plantarum (LP) or LP plus (SU+LP). Silage treated distilled water served as control. After processing for 30 90 d, quality evaluated. composition products pH value were...

Abstract Silages processed with gramineous grass and wood feed mixtures may have positive associative effects on silage quality animal performance when ruminants are fed those silages. The present study was conducted to evaluate the fermentation quality, chemical composition, in vitro rumen digestion characteristics of silages produced sweet sorghum (SS) korshinsk pea shrub (KP) six different ratios fresh weights: 100:0 (0%KP), 80:20 (20%KP), 60:40 (40%KP), 40:60 (60%KP), 20:80 (80%KP),...

High-moisture corn silage presents a novel approach to reduce forage feeding expenses and enhance animal performance. Nevertheless, given corn's proclivity for starch, suboptimal fermentation quality in high-moisture can lead spoilage, posing risks livestock well-being. Therefore, the objective of this study is evaluate effects different additives on profile, chemical composition, nutrient digestibility (HMC) silage. All treatments improved fermentation, as demonstrated by decrease pH...

Sweet sorghum can be used to produce a substantial quantity of biofuel due its high biological yield and carbohydrate content. In this study, we investigated the dynamic changes in fermentation characteristics, components, bacterial community during ensiling wilted unwilted sweet sorghum. The results revealed rapid pattern high-quality quality sorghum, wherein lactic acid, acetic acid accumulated stabilized initial 9 days ensiling, with pH values less than 4.2, until 60 ensiling. We found...

Functional rhizosphere microbiomes (FRM) are critical for plant health and yield. However, the ecological succession of FRM their links to genetic factors across life cycle perennial plants remain poorly understood.

Abstract Leymus chinensis , secalinus and Elymus nutans are three common grasses of the steppes Central Asia. This study examined effects lactic acid bacteria on fermentation qualities aerobic stabilities L. E. silages. The forages were directly ensiled with or without bacteria. Lactobacillus plantarum (LP), brevis (LBR) buchneri NCIMB40788 (LBU) from a commercial inoculant used as additives at 1.0 × 10 5 cfu g −1 fresh matter. control was sprayed same volume distilled water. Six replicates...

Feed use of antioxidants is critical in protecting animals from oxidative stress. Due to consumer resistance synthetic antioxidants, there growing interest using alternative natural substance animal feed industry. The current study aimed evaluate the antioxidant properties peppermint oil, spearmint and scotch oil vitro tests cell culture models. general capacity mint oils was first evaluated through four chemical-based assays including DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging...