A5002871600- Enzyme Production and Characterization
- Enzyme Structure and Function
- Biofuel production and bioconversion
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- Microbial Metabolites in Food Biotechnology
- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Bacterial Genetics and Biotechnology
- Digestive system and related health
- Mesoporous Materials and Catalysis
- Lipid Membrane Structure and Behavior
- Enzyme Catalysis and Immobilization
- Biochemical and Molecular Research
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Carbohydrate Chemistry and Synthesis
- Metal complexes synthesis and properties
- Insect Utilization and Effects
- Biopolymer Synthesis and Applications
- DNA and Nucleic Acid Chemistry
- Insect Resistance and Genetics
- Nanoparticle-Based Drug Delivery
- Endoplasmic Reticulum Stress and Disease
Centre National de la Recherche Scientifique
2010-2025
Université Claude Bernard Lyon 1
2005-2025
CEA Grenoble
2009-2017
Institut de Biologie Structurale
2009-2017
Université Grenoble Alpes
2010-2017
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2009-2017
Centre de Biologie Structurale
2013
Heidelberg University
2008-2010
Université Joseph Fourier
2009-2010
Institut de Biologie et de Chimie des Protéines
2003-2009
The YidC/Oxa1/Alb3 family of membrane proteins controls the insertion and assembly in bacteria, mitochondria, chloroplasts. Here we describe molecular mechanisms underlying interaction Alb3 with chloroplast signal recognition particle (cpSRP). C-terminal domain (A3CT) is intrinsically disordered recruits cpSRP to thylakoid by a coupled binding folding mechanism. Two conserved, positively charged motifs reminiscent chromodomain histone tails are identified A3CT that essential for Alb3-cpSRP43...
Abstract Most membrane proteins studies require the use of detergents, but because lack a general, accurate and rapid method to quantify them, many uncertainties remain that hamper proper functional structural data analyses. To solve this problem, we propose based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) allows quantification pure or mixed detergents in complex with proteins. We validated wide variety automated process, thereby allowing routine for...
Various diseases related to the overconsumption of sugar make a growing need for substitutes. Because sucrose is an inexpensive and readily available d-glucose donor, industrial potential enzymatic synthesis isomers trehalulose and/or isomaltulose from large. The product specificity isomerases that catalyze this reaction depends essentially on possibility tautomerization sucrose, which required formation. For optimal use enzyme, targeting controlled these functional isomers, it necessary...
The mitochondrial ADP/ATP carrier imports ADP from the cytosol into matrix for its conversion to ATP by synthase and exports out of mitochondrion replenish eukaryotic cell with chemical energy. Here substrate specificity human AAC1 was determined two different approaches. In first protein functionally expressed in Escherichia coli membranes as a fusion maltose binding effect excess unlabeled compounds on uptake [(32)P]-ATP measured. second approach cytoplasmic membrane Lactococcus lactis....
Abstract ATP-binding-cassette (ABC) transporters are molecular pumps that translocate molecules across the cell membrane by switching between inward-facing and outward-facing states. To obtain a detailed understanding of their mechanism remains challenge to structural biology, as these proteins notoriously difficult study at level in active, membrane-inserted form. Here we use solid-state NMR investigate multidrug ABC transporter BmrA reconstituted lipids. We identify chemical-shift...
In bacteria the biogenesis of inner membrane proteins requires targeting and insertion factors such as signal recognition particle Sec translocon. YidC is an essential protein involved in together with translocon, but also a separate entity. Escherichia coli member conserved (in bacteria)/Oxa1 mitochondria)/Alb3 chloroplasts) family contains six transmembrane segments large periplasmic domain (P1). We determined crystal structure from E. (P1D) at 1.8 A resolution. The P1D shows...
BmrA from Bacillus subtilis is a half-size ABC (ATP-binding cassette) transporter involved in multidrug resistance. Although its supramolecular organization has been investigated after reconstitution lipid bilayer environment, and shows dimeric possibly tetrameric form, the precise quaternary structure detergent-solubilized state never addressed. In present study, was purified Escherichia coli membranes using an optimized purification protocol different detergents. Furthermore, ATPase...
Stresses increasing the load of unfolded proteins that enter endoplasmic reticulum (ER) trigger a protective response termed protein (UPR). Stromal cell-derived factor2 (SDF2)-type are highly conserved throughout plant and animal kingdoms. In this study we have characterized AtSDF2 as crucial component UPR in Arabidopsis thaliana. Using combination biochemical cell biological methods, demonstrate SDF2 is induced to ER stress conditions causing accumulation proteins. Transgenic reporter...
ABSTRACT Eukaryotic-like membrane Ser/Thr protein kinases play a pivotal role in different aspects of bacterial physiology. In contrast to the diversity their extracellular domains, cytoplasmic catalytic domains are highly conserved. However, function long juxtamembrane domain (JMD), which connects transmembrane helix, remains elusive. this study, we investigated JMD kinase StkP cell division Streptococcus pneumoniae . We observed that deletion affected ability phosphorylate some its...
Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by
Copper (Cu) plays a key role in the photosynthetic process as cofactor of plastocyanin (PC), an essential component chloroplast electron transfer chain. Encoded by nuclear genome, PC is translocated its apo-form into and lumen thylakoids where it processed to mature form acquires Cu. In Arabidopsis, Cu delivery involves two transporters PIB-1 ATPases family, heavy metal associated protein 6 (HMA6) located at envelope HMA8 thylakoid membrane. To gain further insight way delivered PC, we...
Abstract Teichoic acids (TA) are crucial for the homeostasis of bacterial cell wall as well their developmental behavior and interplay with environment. TA can be decorated by different modifications, modulating thus biochemical properties. One major modification consists in esterification d -alanine, a process known -alanylation. -alanylation is performed Dlt pathway, which starts cytoplasm continues extracellularly after -Ala transportation through membrane. In this study, we combined...
ABSTRACT A new cholesterol flow injection analysis biosensor is described. It based on a luminol/hydrogen peroxide electrochemiluminescence (ECL) reaction induced by glassy carbon electrode polarised at + 425 mV vs Pt pseudo reference. The b8543158 sensing layer oxidase (COD) immobilised either UltraBindTM membrane or Immunodyne membrane. physicochemical properties of the type membranes enable high-performance to be obtained. high salt concentration, 3 M NaCl, shown induce 400% increase COD...
The healthy sweetener isomaltulose is industrially produced from the conversion of sucrose by isomerase SmuA Protaminobacter rubrum . Crystal structures in native and deoxynojirimycin complexed forms completed with modeling studies unravel characteristics synthases catalytic pocket their substrate binding mode. Comparison trehalulose synthase MutB highlights role Arg 298 306 active site residues surface charges controlling product specificity isomerases (isomaltulose versus trehalulose)....
Palatinose (isomaltulose, α-d-glucosylpyranosyl-1,6-d-fructofuranose), a nutritional and acariogenic reducing sugar, is industrially obtained from sucrose by using immobilized cells of Protaminobacter rubrum that produce the isomerase SmuA. The isomerization catalyzed this enzyme also results in formation trehalulose (α-d-glucosylpyranosyl-1,1-d-fructofuranose) smaller amounts glucose, fructose eventually isomaltose as by-products, which lower yield reaction complicate recovery palatinose....
The neuronal adaptor protein Fe65 is involved in brain development, Alzheimer disease amyloid precursor (APP) signaling, and proteolytic processing of APP. It contains three protein-protein interaction domains, one WW domain, a unique tandem array phosphotyrosine-binding (PTB) domains. N-terminal PTB domain (Fe65-PTB1) was shown to interact with variety proteins, including the low density lipoprotein receptor-related (LRP-1), ApoEr2 receptor, histone acetyltransferase Tip60. We have...
The mitochondrial ADP/ATP carrier (AAC) is a prominent actor in the energetic regulation of cell, importing ADP into mitochondria and exporting ATP toward cytoplasm. Severe genetic diseases have been ascribed to specific mutations this membrane protein. How minute, well-localized modifications transporter impact function remains, however, largely unclear. Here, for first time, relationship between all documented pathological AAC its transport properties established. Activity measurements...
Copper is a crucial ion in cells, but needs to be closely controlled due its toxic potential and ability catalyse the formation of radicals. In chloroplasts, an important step for proper functioning photosynthetic electron transfer chain delivery copper plastocyanin thylakoid lumen. The main route transport lumen driven by two PIB-type ATPases, Heavy Metal ATPase 6 (HMA6) HMA8, located inner membrane chloroplast envelope membrane, respectively. Here, crystal structures nucleotide binding...
Sucrose isomerase is an enzyme that catalyzes the production of sucrose isomers high biotechnological and pharmaceutical interest. Owing to complexity chemical synthesis these isomers, isomaltulose trehalulose, enzymatic conversion remains preferred method for obtaining products. Depending on microbial source, ratio sucrose-isomer products varies significantly. In studies aimed at understanding explaining underlying molecular mechanisms reactions, mutations obtained using a...
ABSTRACT The psychrophilic alkaline metalloprotease (PAP) produced by a Pseudomonas bacterium isolated in Antarctica belongs to the clan of metzincins, for which zinc ion is essential catalytic activity. Binding studies crystalline state have been performed X-ray crystallography order improve understanding role and calcium ions bound this protease. Cocrystallization soaking experiments with EDTA concentration range from 1 85 mM resulted five three-dimensional structures distinct number metal...