A5102923149- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Cholinesterase and Neurodegenerative Diseases
- Computational Drug Discovery Methods
- Analytical Chemistry and Sensors
- Microfluidic and Capillary Electrophoresis Applications
- Retinal Development and Disorders
- Advanced Biosensing Techniques and Applications
- Medicinal Plants and Neuroprotection
- Advanced biosensing and bioanalysis techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrochemical sensors and biosensors
- Marine Sponges and Natural Products
- Electrochemical Analysis and Applications
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Pluripotent Stem Cells Research
- Cardiac electrophysiology and arrhythmias
- Axon Guidance and Neuronal Signaling
- Cell Image Analysis Techniques
- Nanofabrication and Lithography Techniques
- Electrical and Bioimpedance Tomography
Karlsruhe Institute of Technology
2022-2024
Leipzig University
2013-2022
Fraunhofer Institute for Biomedical Engineering
1999-2008
Technical University of Darmstadt
1997-2005
Fraunhofer Society
2001-2002
German Cancer Research Center
1993-1994
Heidelberg University
1994
Johannes Gutenberg University Mainz
1989-1992
Darmstadt University of Applied Sciences
1988
Abstract The β‐site amyloid precursor protein‐cleaving enzyme 1 (BACE1) is a prerequisite for the generation of β‐amyloid peptides, principle constituents senile plaques in brains patients with Alzheimer's disease (AD). BACE1 expression and enzymatic activity are increased AD brain, but regulatory mechanisms largely unknown. Here we show that Yin Yang (YY1), highly conserved multifunctional transcription factor, binds to its putative recognition sequence within promoter stimulates rat...
Close to realistic responses anti-cancer drugs are not adequately provided in monolayer or single cells assays. 3-dimensional multicellular cultures (spheroids) mimicking vivo-like conditions established as cell biological models for microtumors/metastases. For a non-invasive real-time monitoring of the electrical parameters such spheroid we designed, fabricated and tested 3D multifunctional electrode-based microcavity array. In non-adherent assay acute tests with tumor spheroids were done...
Human casein kinase II (CKII) is a ubiquitous and multipotential Ser/Thr involved in the regulation of cell growth differentiation. Biochemically, two characteristics are particularly notable; first, tetrameric composition catalytic subunits (α and/or α′) regulatory (β); second, autophosphorylation holoenzyme at N‐terminus CKIIβ, suspected to be tuning activity. Whether CKIIα CKIIα′ reconstitute comparably with CKIIβ form unclear. For systematic investigation, complete set recombinant CKII...
In today's neurodevelopment and -disease research, human neural stem/progenitor cell-derived networks represent the sole accessible in vitro model possessing a primary phenotype. However, cultivation moreover, differentiation as well maturation of cells are very complex time-consuming processes. Therefore, techniques for sensitive non-invasive, real-time monitoring neuronal highly demanded. Using impedance spectroscopy, several cell lines was analyzed detail. After development an optimum...
Cancer resistance is a major cause for longevity of the naked mole-rat. Recent liver transcriptome analysis in this animal compared to wild-derived mice revealed higher expression alpha2-macroglobulin (A2M) and cell adhesion molecules, which contribute mole-rat's cancer resistance. Notably, A2M known dramatically decrease with age humans. We hypothesize that might facilitate tumour development. Here we found modulates adhesion, migration growth by inhibition promoting signalling pathways,...
Abstract Large‐ring cyclodextrins (CD) are cyclic glucans composed of 9 or more α‐1,4‐linked glucose units. They minor side products bacterial glucanotransferases (CGTases, EC 2.4.1.19) and have previously been available only in very small amounts for studies their properties supramolecular complex formation reactions. We engineered a CGTase to synthesize mainly large‐ring CD facilitating preparation larger amounts. By reversed phase chromatography, we obtained single samples 10 12 units...
Tauopathies including Alzheimer's disease represent one of the major health problems aging population worldwide. Therefore, a better understanding tau-dependent pathologies and consequently, tau-related intervention strategies is highly demanded. In recent years, several tau-focused therapies have been proposed with aim to stop progression. However, develop efficient active pharmaceutical ingredients for broad treatment patients, further improvements are necessary detailed neurodegenerative...
Human pluripotent stem cell derived cardiomyocytes are a promising source for research and clinical applications like investigation of cardiomyopathies therefore, identification testing novel therapeutics as well based therapy approaches. However, actually it´s challenge to generate matured adult cardiomyocyte-like phenotype in reasonable time. Moreover, there is lack applicable non-invasive label-free monitoring techniques providing quantitative parameters analysing the culture stability...
Chip-integrated electrically connected stationary SERS targets enable interference-free consecutive surface-enhanced Raman measurements by assisted substrate regeneration.
Tauopathies such as Alzheimer's disease (AD) belong to the group of neurodegenerative diseases that are characterised by hyperphosphorylation protein tau. Hyperphosphorylation tau is one salient events leading neuronal cytotoxicity and cognitive impairments. In this context, inhibition specific kinase inhibitors can provide an excellent drug target for treatment AD other tau-related diseases. To improve identification, optimisation validation during high-cost hit-to-lead cycle drugs, we...
Cardiovascular diseases represent the most common cause of death in industrialized countries. In this context vascular smooth muscle cells (SMCs) are a major key player that is involved pathological processes like hypertension and atherosclerosis. Therefore pharmaceutical industry intensively investigated developing non-destructive label-free monitoring techniques for quantitative detection SMC characteristics field active development as well clinical diagnostics. Hence, we developed novel...
Unexpected adverse effects on the cardiovascular system remain a major challenge in development of novel active pharmaceutical ingredients (API). To overcome current limitations animal-based vitro and vivo test systems, stem cell derived human cardiomyocyte clusters (hCMC) offer opportunity for highly predictable pre-clinical testing. The three-dimensional structure hCMC appears more representative tissue milieu than traditional monolayer culture. However, there is lack long-term, real time...
The maximum response and 10-year survival rate for metastatic melanoma patients treated with standardised chemotherapy is still less than 15% 10%, respectively. In contrast, oncogene targeting was found a promising tool killing of BRAFV600 mutated cells. Nevertheless, despite improved rates, resistance acquisition remains an ongoing problem. this context, the impact chronic BRAF inhibition on efficacy commonly applied cytostatics unknown. our study, human cells BRAFV600E mutation were...