A5022209101- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Cardiomyopathy and Myosin Studies
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Cardiac Fibrosis and Remodeling
- Ubiquitin and proteasome pathways
- Reproductive Biology and Fertility
- Renal and related cancers
- Neurogenetic and Muscular Disorders Research
- Cardiac Structural Anomalies and Repair
- Knee injuries and reconstruction techniques
- Pulmonary Hypertension Research and Treatments
- Wnt/β-catenin signaling in development and cancer
- Adipose Tissue and Metabolism
- Genetic Syndromes and Imprinting
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Telomeres, Telomerase, and Senescence
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
Sorbonne Université
2010-2022
Inserm
2013-2022
Unité de recherche sur les maladies cardiovasculaires et métaboliques
2015-2022
Paris Cardiovascular Research Center
2018-2022
Université Paris Cité
1989-2022
University of California, San Francisco
2020
Fondation pour l’innovation en Cadiométabolisme et Nutrition
2016-2018
Université Sorbonne Paris Nord
2017
Centre Chirurgical Marie Lannelongue
2016
Centre National de la Recherche Scientifique
1990-2016
ABSTRACT The int-3 oncogene was identified as a frequent target in Mouse Mammary Tumor Virus (MMTV)-induced mammary carcinomas and encodes the intracellular domain of novel mouse Notch gene. To investigate role proto-oncogene development carcinogenesis, we isolated cDNA clones corresponding to entire coding potential proto-oncogene. We propose name this gene Notch4 reserve nomenclature for references oncogenic form. deduced amino acid sequence contains conserved motifs found proteins;...
Expression of the two isoforms cardiac myosin heavy chain (MHC), MHC alpha and beta, in mammals is regulated postnatally by a variety stimuli, including serum hormone levels. Less known about factors that regulate gene expression rapidly growing muscle embryos. Using isoform-specific 35S-labeled cRNA probes corresponding to genes alkali light (MLC) expressed muscle, we have investigated temporal spatial pattern these different developing mouse heart situ hybridization. Between 7.5 8 d post...
B R Stanton, A S Perkins, L Tessarollo, D Sassoon, and F Parada Molecular Embryology Section, National Cancer Institute-Frederick Research Development Center, Maryland 21702-1201.
ABSTRACT The murine female reproductive tract differentiates along the anteroposterior axis during postnatal development. This process is marked by emergence of distinct cell types in oviduct, uterus, cervix and vagina dependent upon specific mesenchymal-epithelial interactions as demonstrated earlier heterografting experiments. Members Wnt family signaling molecules have been recently identified this system an early functional role development has demonstrated. Mice were generated using...
Among the first tissues to differentiate in mammalian embryo are cardiac and subsequently skeletal striated muscle. We have developed specific cRNA probes corresponding 5' noncoding regions of alpha-cardiac alpha-skeletal actin mRNAs order investigate myogenesis mouse embryo. Transcripts coding for which is major isoform adult heart can be detected between 7.5 7.8 days p.c. developing observed all somites as they formed. In addition, transcripts accumulated at much lower levels tissue newly...
The muscle regulatory factors MRF4, myogenin, myf-5, and MyoD constitute a family of proteins that can function as muscle-specific transcriptional activators. Although this gene has been extensively studied, specific role for each factor during myogenesis remains to be determined. Understanding how these requires detailed analysis their expression patterns development. Toward goal, we examined the temporal pattern MRF4 other in rat myogenic cell line L6J1-C, newborn primary cultures fetal...
Myogenic helix-loop-helix (HLH) proteins, such as myogenin and MyoD, can activate muscle-specific transcription when introduced into a variety of nonmuscle cell types. Whereas cells mesodermal origin are especially permissive to the actions these myogenic regulators, many other types refractory conversion by them. Here we describe novel homeodomain protein, MHox, that binds an A+T-rich element in muscle creatine kinase (MCK) enhancer is essential for trans-activation HLH proteins. MHox...
Using in situ hybridization, we have investigated the temporal sequence of myosin gene expression developing skeletal muscle masses mouse embryos. The probes used were isoform-specific, 35S-labeled antisense cRNAs to known sarcomeric heavy chain and alkali light transcripts. Results showed that both cardiac mRNAs first detected between 9 10 d post coitum (p.c.) myotomes most rostral somites. Myosin transcripts appeared more caudal somites at later stages a developmental gradient. earliest...
ABSTRACT The cardiac conduction system is a complex network of cells that together orchestrate the rhythmic and coordinated depolarization heart. molecular mechanisms regulating specification patterning form this conductive are largely unknown. Studies in avian models have suggested components arise from progressive recruitment cardiomyogenic progenitors, potentially influenced by inductive effects neighboring coronary vasculature. However, relatively little known about process development...
Epithelial-mesenchymal interactions play a crucial role in the correct patterning of mammalian female reproductive tract (FRT). Three members Wnt family growth factors are expressed at high levels developing FRT mouse embryo. The expression genes is maintained adult FRT, although fluctuate during estrous. Wnt4 required for Müllerian duct initiation, whereas Wnt7a subsequent differentiation. In this study, we show that Wnt5a posterior FRT. We further demonstrate mutant has potential to form...
ABSTRACT We report that during mouse fetal development transcripts of Msx1 and Msx2 become progressively restricted to cells will form more distal digit structures; the expression domain is always than Msx1. At birth both are expressed in nail bed hair follicle. have found regenerative ability tips levels which amputation plane within region Msx1, but not Msx2, early digits where late neonatal digits. Fetal tip regeneration rapid completed by birth, whereas requires 4 weeks sometimes...
In the mammalian cochlea, stereociliary bundles located on mechanosensory hair cells within sensory epithelium are unidirectionally oriented. Development of this planar polarity is necessary for normal hearing as only sensitive to vibrations in a single plane; however, mechanisms governing their orientation unknown. We report that Wnt signaling regulates development unidirectional bundle orientation. vitro application Wnt7a protein or inhibitors signaling, secreted Frizzled-related 1...
The satellite cell is the major tissue-resident stem underlying muscle regeneration; however, multiple non-satellite myogenic progenitors as well non-myogenic populations that support regenerative process have been identified. PW1 expressed in cells a subset of interstitial with potential termed PICs (PW1+ cells). Microarray profiling revealed express broad range genes common to mesenchymal cells, whereas consistent committed progenitor. Isolated from both young and adult muscles can...
Fibro-adipogenic progenitors (FAPs) are an interstitial cell population in adult skeletal muscle that support regeneration. During development, connective tissue (MCT) cells proper patterning, however the underlying molecular mechanisms not well understood and it remains unclear whether FAPs embryonic MCT share a common lineage. We show here mouse limb expressing transcription factor Osr1, differentiate into fibrogenic adipogenic vivo vitro defining FAP-like population. Genetic lineage...
Environmental factors during early life are critical for the later metabolic health of individual and future progeny. In our obesogenic environment, it is great socioeconomic importance to investigate mechanisms that contribute risk ill health. Imprinted genes, a class functionally mono-allelic genes growth axis development, have been proposed be uniquely susceptible environmental change. Furthermore, has also suggested perturbation epigenetic reprogramming imprinting control regions (ICRs)...
Abstract Mutations in amphiphysin‐2/ BIN 1, dynamin 2, and myotubularin are associated with centronuclear myopathy ( CNM ), a muscle disorder characterized by myofibers atypical central nuclear positioning abnormal triads. Mis‐splicing of 1 is also myotonic dystrophy that shares histopathological hallmarks . How amphiphysin‐2 orchestrates triad organization how ‐associated mutations lead to dysfunction remains elusive. We find N‐ WASP interacts this interaction distribution disrupted...