A5102995333- Osteoarthritis Treatment and Mechanisms
- Natural product bioactivities and synthesis
- Cancer-related molecular mechanisms research
- Adipokines, Inflammation, and Metabolic Diseases
- Protease and Inhibitor Mechanisms
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Medicinal plant effects and applications
- Tendon Structure and Treatment
- Chemokine receptors and signaling
- Inflammatory mediators and NSAID effects
- Bioactive Compounds in Plants
- Orthopedic Infections and Treatments
- Immune Response and Inflammation
- Regulation of Appetite and Obesity
- Bone Metabolism and Diseases
- Orthopaedic implants and arthroplasty
- Estrogen and related hormone effects
- Knee injuries and reconstruction techniques
- Hippo pathway signaling and YAP/TAZ
- Hormonal and reproductive studies
- Hemophilia Treatment and Research
- Physical Education and Pedagogy
- Sesquiterpenes and Asteraceae Studies
- Developmental Biology and Gene Regulation
Shanxi Medical University
2025
Guangzhou Sport University
2024
Second Affiliated Hospital of Zhejiang University
2014-2024
Zhejiang University
2006-2024
Zunyi Medical University
2024
Guangzhou University
2020-2024
Daping Hospital
2019-2023
Army Medical University
2010-2023
Guiyang Medical University
2022
Foundation for Human Potential
2019
Interleukin 18 (IL-18) promotes inflammation and apoptosis in chondrocytes, thereby contributing to the development progression of osteoarthritis (OA). Here, we investigated effects IL-18 treatment inhibition rat chondrocytes vitro vivo. We used RT-PCR Western blotting measure mRNA protein levels chondrocyte-specific genes Collagen II Aggrecan as well apoptosis-related (Bax, Bcl2, Caspase3/9), autophagy-related (Atg5, Atg7, Beclin1, LC3), mTOR pathway-related (PI3K, Akt, mTOR). observed a...
Abstract Iron metabolism dysregulation is tightly associated with cancer development. But the underlying mechanisms remain poorly understood. Increasing evidence has shown that long noncoding RNAs (lncRNAs) participate in various metabolic processes via integrating signaling pathway. In this study, we revealed one iron-triggered lncRNA, target of YAP, LncRIM (LncRNA Related to Metabolism, also named ZBED5-AS1 and Loc729013 ), which effectively links Hippo pathway iron largely independent on...
Introduction: Osteoarthritis (OA) is the most prevalent joint disorder in elderly population, and inflammatory mediators like IL-1βwere thought to play central roles its development. Schisandrin B, main active component derived from Schisandra chinensis , exhibited antioxidative antiinflammatory properties. Methods: In present study, protective effect underlying mechanism of B on OA was investigated vivo vitro. Results: The results showed that decreased IL-1β-induced upregulation matrix...
Metabolic disorders and inflammation of chondrocytes are major pathological changes in aging cells osteoarthritis (OA). Recent studies demonstrated age-related mitochondrial dysfunction may be a key contributing factor the development OA. Mitofusin 2 (MFN2) is regulator fusion, cell metabolism, autophagy apoptosis. This study was performed to ascertain whether MFN2 involved OA.Metabolic measurements were taken rat between different ages (3-week, 5-month, 12-month). activity detected both...
This study aims to investigate the role and mechanism of FGFR3 in macrophages their biological effects on pathology arthritis.Mice with conditional knockout myeloid cells (R3cKO) were generated. Gait behaviours mice monitored at different ages. Spontaneous synovial joint destruction was evaluated by digital radiographic imaging μCT analysis; changes articular cartilage synovitis determined histological analysis. The recruitment synovium examined immunostaining monocyte trafficking assay....
Previous studies of the direct actions bisphosphonates on bone have mainly been limited to their effects bone-resorbing osteoclasts and little is known about osteoblasts. Here we report alendronate proliferation osteogenic differentiation MG-63 osteoblast-like cell line. Cell was determined with MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, evaluated an alkaline phosphatase bioassay by analysis gene expression reverse transcription-polymerase chain reaction,...
Osteoarthritis (OA) is a chronic degenerative joint disease, related to the overexpression of matrix metalloproteinases (MMPs), disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), inflammation, chondrocyte apoptosis. Nesfatin-1 an adipokine, which plays important role in development OA, especially obese people. In present study, cartilage degradation apoptosis observed OA patients was evaluated. Furthermore, anti-inflammatory anti-apoptotic effects nesfatin-1, its...
To explore the influence of different background factors on middle school PE teachers' self-efficacy, work input and creative teaching, to reveal relationship between teaching self-efficacy teaching.By means engagement scale, a questionnaire survey was conducted among teachers, data were processed modeled by SPSS AMOS statistical analysis software.Physical education (PE) self-teaching effectiveness influenced such as gender, age, full-time or part-time educational level. Work only affected...
Rosmarinic acid (RosA) is a water-soluble polyphenol, which can be isolated from many herbs such as orthosiphon diffuses and rosmarinus officinalis. Previous studies have shown that RosA possesses various biological properties. In this study, we investigate the anti-osteoarthritic effects of in rat articular chondrocytes. Chondrocytes were pre-treated with RosA, followed by stimulation IL-1β. Real-time PCR Western blot performed to detect expression matrix metalloproteinase (MMP)-1, MMP-3...
Abstract Diabetes mellitus (DM) is one of the prominent risk factors for pathological development and progression tendinopathy. One feature DM‐related changes in tendinopathy accumulation advanced glycation end products (AGEs) affected tendons. Pioglitazone (Pio), a peroxisome proliferator‐activated receptor γ agonist, performs protective effect against AGEs. The present study aimed to investigate pathogenetic role AGEs on tendon‐derived stem cells (TDSCs) determine Pio AGEs‐induced TDSC...
Osteoarthritis (OA) is the most prevalent joint disease and uncontrolled inflammation now recognized to play vital roles in OA development. The orphan nuclear receptor 4A1(NR4A1) a key negative regulator of inflammatory responses but its role osteoarthritis remains unclear. In present study, we found that NR4A1 expression was elevated human cartilage vitro model, which could be blocked by NF-κB signal inhibitor JSH23. overexpression inhibited, whereas knockdown enhanced IL-1β induced COX-2,...
<i >Background</i>. Adipokines have been proved to relate with osteoarthritis (OA). As a recently discovered adipokine, nesfatin-1 relationship OA has not reported. >Aim</i>. To determine the levels of in serum and synovial fluid (SF) from patients without OA; examine correlation between high sensitivity C-reactive protein (hsCRP), Type IIA Collagen N Propeptide (PIIANP), IL-18 (interleukin-18) or fluid. >Methods</i>. Serum SF were collected knee healthy persons, respectively. Five articular...
Tendinopathy is a common musculoskeletal disorder, mainly affects the athletes and aged people. Tumor necrosis factor-α (TNF-α) plays an initiator role in tendinopathy. Tectorigenin extract component of Belam-canda Chinesis, which performs anti-inflammatory anti-apoptosis effects. The present study stablished to investigate tectorigenin against pathogenetic effects TNF-α on tendon-derived stem cells (TDSCs) vivo vitro. findings indicated that able induce TDSC inflammation, apoptosis...
Heterotopic ossification (HO) is a pathological condition involved in tendinopathy. Adipokines are known to play key role HO of Nesfatin-1, an 82-amino acid adipokine closely reportedly associated with diabetes mellitus (DM), which, turn, related In the present study, we aimed investigate effects nesfatin-1 on osteogenic differentiation tendon-derived stem cells (TDSCs) and pathogenesis tendinopathy rats. vitro , TDSCs were incubated induction medium for 14 days different concentration. vivo...
Background: Baicalein is a flavonoid isolated from Scutellaria baicalensis Georgi. Here, we investigated the anti-osteoarthritic effect of baicalein in vitro and vivo. Methods: Interleukin-1 beta (IL-1β)-induced chondrocytes were treated with different concentrations baicalein, real-time PCR ELISA performed to detect matrix metalloproteinases (MMPs) expression. Western blot was used evaluate mitogen-activated protein kinase (MAPK) In experimental osteoarthritis (OA), rabbits gross...
Tricetin is a well-studied flavonoid with wide range of pharmacological activities in cancer and inflammation. However, the ability tricetin to ameliorate inflammation that occurs osteoarthritis (OA) has not been determined. This study explored effects on interleukin- (IL-) 1 β -induced rat chondrocytes. Chondrocytes harvested from cartilage were incubated vitro presence IL-1 . The expression matrix metalloproteinase- (MMP-) 1, MMP-3, MMP-13, nitric oxide (NO), prostaglandin E2 (PGE2), Bax,...
Synovial inflammation is a major pathological feature of osteoarthritis (OA), which chronic degenerative joint disease. Fibroblast-like synoviocytes (FLS), localized in the synovial membrane, are specialized secretory cells. During OA synovitis, FLS produce chemokines and cytokines that stimulate chondrocytes to secrete inflammatory activate matrix metalloproteinases (MMPs) FLS. Recent studies have demonstrated sirtuin 3 (SIRT3) performs as key regulator maintaining mitochondrial homeostasis...
Summary. Advances in therapy have improved life expectancy and quality of patients with haemophilia A. Due to the chronic complex management this disease, particularly, development inhibitors, little is known about their health resource utilization real‐life setting over time. The aim was assess distribution trend healthcare among US A without inhibitors. MarketScan® Database, queried identify individuals ≥1 year continuous enrolment, two medical diagnoses claims for factor VIII or bypassing...