A5030633317- Mycobacterium research and diagnosis
- Phytoestrogen effects and research
- Soybean genetics and cultivation
- Tuberculosis Research and Epidemiology
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Botanical Research and Chemistry
- Cancer therapeutics and mechanisms
- Ruminant Nutrition and Digestive Physiology
- vaccines and immunoinformatics approaches
- Diagnosis and treatment of tuberculosis
Washington University in St. Louis
2018-2023
National Museum of Natural History
2018
Smithsonian Institution
2018
Mycobacterium tuberculosis ( Mtb ) killed more people in 2017 than any other single infectious agent. This dangerous pathogen is able to withstand stresses imposed by the immune system and tolerate exposure antibiotics, resulting persistent infection. The global (TB) epidemic has been exacerbated emergence of mutant strains that are resistant frontline antibiotics. Thus, both phenotypic drug tolerance genetic resistance major obstacles successful TB therapy. Using a chemical approach...
The ability to regulate gene expression through transcription initiation underlies the adaptability and survival of all bacteria. Recent work has revealed that machinery in many bacteria diverges from paradigm been established Escherichia coliMycobacterium tuberculosis (Mtb) encodes RNA polymerase (RNAP)-binding protein CarD, which is absent E. coli but required form stable RNAP-promoter open complexes (RPo) essential for viability Mtb stabilization RPo by CarD proposed result activation...
Bacterial pathogens like Mycobacterium tuberculosis (Mtb) employ transcription factors to adapt their physiology the diverse environments within host. CarD is a conserved bacterial factor that essential for viability in Mtb. Unlike classical recognize promoters by binding specific DNA sequence motifs, binds directly RNA polymerase stabilize open complex intermediate (RP
Kudzu, Pueraria montana var. lobata, is a woody vine native to Southeast Asia that has been introduced globally for cattle forage and erosion control. The highly invasive in its areas, including the southeastern US. Modern molecular marker resources are limited species, despite importance. Transcriptomes P. lobata second phaseoloid legume taxon previously ascribed genus Pueraria, Neustanthus phaseoloides, were generated mined microsatellites single nucleotide polymorphisms.Roche 454...
RNA polymerase (RNAP) binding protein A (RbpA) is essential for mycobacterial viability and regulates transcription initiation by increasing the stability of RNAP-promoter open complex (RPo). RbpA consists four domains: an N-terminal tail (NTT), a core domain (CD), basic linker, sigma interaction domain. We have previously shown that truncation NTT CD increases RPo stabilization RbpA, implying these domains inhibit this activity RbpA. Previously published structural studies showed are...
Bacterial pathogens like Mycobacterium tuberculosis ( Mtb ) employ transcription factors to adapt their physiology the diverse environments within host. CarD is a conserved bacterial factor that essential for viability in . Unlike classical recognize promoters by binding specific DNA sequence motifs, binds directly RNA polymerase (RNAP) stabilize open complex intermediate (RP o during initiation. We previously showed using RNA-sequencing capable of both activating and repressing vivo...