A5011243866- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Autophagy in Disease and Therapy
- Mycobacterium research and diagnosis
- Vibrio bacteria research studies
- Phagocytosis and Immune Regulation
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Blood disorders and treatments
- vaccines and immunoinformatics approaches
- Cancer therapeutics and mechanisms
- interferon and immune responses
- Adenosine and Purinergic Signaling
- Cell death mechanisms and regulation
- Sarcoidosis and Beryllium Toxicity Research
- Salmonella and Campylobacter epidemiology
- Pneumonia and Respiratory Infections
- Soil Carbon and Nitrogen Dynamics
- Antifungal resistance and susceptibility
- Extracellular vesicles in disease
Emory University
2025
Washington University in St. Louis
2016-2023
Institute for Women's Policy Research
2023
University of Alberta
2013-2017
Alberta Glycomics Centre
2013-2014
Abstract Chemical synthesis of conjugate vaccines, consisting a polysaccharide linked to protein, can be technically challenging, and in vivo bacterial conjugations (bioconjugations) have emerged as manufacturing alternatives. Bioconjugation relies upon an oligosaccharyltransferase attach polysaccharides proteins, but currently employed enzymes are not suitable for the generation vaccines when contain glucose at reducing end, which is case ~75% Streptococcus pneumoniae capsules. Here, we use...
The immune response to Mycobacterium tuberculosis infection determines disease outcomes, yet we have an incomplete understanding of what factors contribute a protective response. Neutrophilic inflammation has been associated with poor prognosis in humans and animal models during M. and, therefore, must be tightly regulated. ATG5 is essential autophagy protein that required innate cells control neutrophil-dominated promote survival infection; however, the mechanistic basis for how regulates...
Multiple species within the Acinetobacter genus are nosocomial opportunistic pathogens of increasing relevance worldwide. Among virulence factors utilized by these bacteria type IV pili and a protein O-glycosylation system. Glycosylation is mediated O-oligosaccharyltransferases (O-OTases), enzymes that transfer glycan from lipid carrier to target proteins. difficult identify due similarities with WaaL ligases catalyze last step in lipopolysaccharide synthesis. A bioinformatics analysis...
Acinetobacter baumannii, A. nosocomialis, and pittii have recently emerged as opportunistic human pathogens capable of causing severe disease; however, the molecular mechanisms employed by to cause disease remain poorly understood. Many pathogenic members genus contain genes predicted encode proteins required for biogenesis a type II secretion system (T2SS), which been shown mediate virulence in many Gram-negative organisms. Here we demonstrate that nosocomialis strain M2 produces functional...
Pathogenic Acinetobacter species, including baumannii and nosocomialis, are opportunistic human pathogens of increasing relevance worldwide. Although their mechanisms drug resistance well studied, the virulence factors that govern pathogenesis incompletely characterized. Here we define complete secretome A. nosocomialis strain M2 in minimal medium demonstrate pathogenic species produce both a functional type I secretion system (T1SS) contact-dependent inhibition (CDI) system. Using...
Mycobacterium tuberculosis ( Mtb ) killed more people in 2017 than any other single infectious agent. This dangerous pathogen is able to withstand stresses imposed by the immune system and tolerate exposure antibiotics, resulting persistent infection. The global (TB) epidemic has been exacerbated emergence of mutant strains that are resistant frontline antibiotics. Thus, both phenotypic drug tolerance genetic resistance major obstacles successful TB therapy. Using a chemical approach...
The opportunistic human pathogen Acinetobacter baumannii is a concern to health care systems worldwide because of its persistence in clinical settings and the growing frequency multiple drug resistant infections. To combat this threat, it necessary understand factors associated with disease environmental A. baumannii. Recently, was shown that single biosynthetic pathway responsible for generation capsule polysaccharide O-linked protein glycosylation. Because requirement these carbohydrates...
,
Antibiotic resistance is a global crisis that threatens our ability to treat bacterial infections, such as tuberculosis, caused by Mycobacterium tuberculosis Of the 10 million cases of in 2017, approximately 19% new and 43% previously treated were strains M. resistant at least one frontline antibiotic. There clear need for therapies target these genetically strains. Here, we report discovery series antimycobacterial compounds, 4-amino-thieno[2,3-d]pyrimidines, potently inhibit growth To...
Polymorphisms in the IRGM gene are associated with susceptibility to tuberculosis humans. A murine ortholog of Irgm, Irgm1, is also essential for controlling Mycobacterium (Mtb) infection mice. Multiple processes have been IRGM1 activity that could impact host response Mtb infection, including roles autophagy-mediated pathogen clearance and expansion activated T cells. However, what IRGM1-mediated pathway necessary control vivo mechanistic basis this remains unknown. We dissected...
Neutrophils are the most abundant cell type in airways of tuberculosis patients. Mycobacterium (Mtb) infection induces release neutrophil extracellular traps (NETs); however, molecular regulation and impact NET on Mtb pathogenesis unknown. We find that during neutrophils, PAD4 citrullinates histones to decondense chromatin gets released as NETs a manner can maintain viability promote replication. Type I interferon promotes formation chromatin-containing vesicles allow without compromising...
Polymorphisms in the
SUMMARY Neutrophils are the most abundant cell type in airways of tuberculosis patients. Recent investigations reported induction neutrophil extracellular traps (NETs) during Mycobacterium ( Mtb ) infection, however, molecular regulation and impact NETosis on pathogenesis is unknown. We find that response to infection neutrophils, PAD4 citrullinates histones decondense chromatin gets packaged into vesicles for release as NETs a manner can maintain viability promote replication. Type I...
SUMMARY Inflammation is critical for controlling infections, but when left unchecked can cause tissue damage and disease. For tuberculosis, the leading of death due to infection 1 , host inflammation responsible clinical symptoms 2 morbidity mortality 3,4 . Specifically, neutrophil-dominated associated with tuberculosis disease progression 3,5,6 Therefore, understanding how neutrophil functions are regulated during important developing ways prevent Atg5 was first gene shown specifically...
Summary Host autophagy had been associated with the control of Mycobacterium tuberculosis (Mtb) infection due to its ability sequesters microorganisms through a process termed “xenophagy” 1–4 . Xenophagy purportedly limits Mtb replication within infected macrophages However, studies in mice using standard low-dose model demonstrated that xenophagy phagocytes is not required pathogenesis 5,6 Instead, an autophagy-independent function ATG5 myeloid cells controls limiting neutrophilic...