A5059495334- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Immune cells in cancer
- interferon and immune responses
- Autophagy in Disease and Therapy
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Phagocytosis and Immune Regulation
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Blood disorders and treatments
- Endometriosis Research and Treatment
- Antifungal resistance and susceptibility
- Herbal Medicine Research Studies
- Sarcoidosis and Beryllium Toxicity Research
- Endometrial and Cervical Cancer Treatments
- Essential Oils and Antimicrobial Activity
- Histone Deacetylase Inhibitors Research
- PARP inhibition in cancer therapy
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Bioactive Compounds and Antitumor Agents
- Respiratory and Cough-Related Research
Institute for Women's Policy Research
2023
Washington University in St. Louis
2021-2023
University of Washington
2023
KIIT University
2014-2021
Research Center Borstel - Leibniz Lung Center
2020
Endometriosis is a pathological condition of the female reproductive tract characterized by existence endometrium-like tissue at ectopic sites, affecting 10% women between age 15 and 49 in USA. However, currently there no reliable non-invasive method to detect presence endometriosis without surgery many find hormonal therapy as ineffective avoiding recurrences. There lack knowledge on etiology factors that contribute development endometriosis. A growing body recent evidence suggests an...
Artemisia nilagirica (Asteraceae) and Murraya koenigii (Rutaceae) are widely distributed in eastern region of India. Leaves plant used to treat cold cough by the local tribal population east is an edible previously reported have antibacterial activity. Pathogenic strains mycobacteria resistant most conventional antibiotics. Therefore, it imperative identify novel antimycobacterial molecules mycobacterial infection.In this study, ethanol, petroleum ether water extracts were tested for...
Polymorphisms in the IRGM gene are associated with susceptibility to tuberculosis humans. A murine ortholog of Irgm, Irgm1, is also essential for controlling Mycobacterium (Mtb) infection mice. Multiple processes have been IRGM1 activity that could impact host response Mtb infection, including roles autophagy-mediated pathogen clearance and expansion activated T cells. However, what IRGM1-mediated pathway necessary control vivo mechanistic basis this remains unknown. We dissected...
Neutrophils are the most abundant cell type in airways of tuberculosis patients. Mycobacterium (Mtb) infection induces release neutrophil extracellular traps (NETs); however, molecular regulation and impact NET on Mtb pathogenesis unknown. We find that during neutrophils, PAD4 citrullinates histones to decondense chromatin gets released as NETs a manner can maintain viability promote replication. Type I interferon promotes formation chromatin-containing vesicles allow without compromising...
ABSTRACT Mycobacterium tuberculosis primarily infects lung macrophages. However, a recent study showed that M. also and persists in dormant form inside bone marrow mesenchymal stem cells (BM-MSCs) even after successful antibiotic therapy. the mechanism(s) by which survives BM-MSCs is still not known. Like macrophages, do contain well-defined endocytic pathway, known to play central role clearance of internalized mycobacteria. Here, we studied fate virulent avirulent mycobacteria Sca-1 + CD44...
Mycobacterium tuberculosis subverts host immunity to proliferate within tissues. Non-selective transient receptor potential (TRP) ion channels are involved in responses and altered upon bacterial infections. Altered expression localization of TRPV4 macrophages virulent M. infection together with differential distribution human (TB) granulomas indicate a role TB. Compared wild-type mice, Trpv4-deficient littermates showed transiently higher mycobacterial burden reduced proinflammatory...
Polymorphisms in the
SUMMARY Neutrophils are the most abundant cell type in airways of tuberculosis patients. Recent investigations reported induction neutrophil extracellular traps (NETs) during Mycobacterium ( Mtb ) infection, however, molecular regulation and impact NETosis on pathogenesis is unknown. We find that response to infection neutrophils, PAD4 citrullinates histones decondense chromatin gets packaged into vesicles for release as NETs a manner can maintain viability promote replication. Type I...
Summary Host autophagy had been associated with the control of Mycobacterium tuberculosis (Mtb) infection due to its ability sequesters microorganisms through a process termed “xenophagy” 1–4 . Xenophagy purportedly limits Mtb replication within infected macrophages However, studies in mice using standard low-dose model demonstrated that xenophagy phagocytes is not required pathogenesis 5,6 Instead, an autophagy-independent function ATG5 myeloid cells controls limiting neutrophilic...
Abstract Tuberculosis, caused by Mycobacterium tuberculosis (Mtb), remains a critical health issue. Xenophagy, the targeting of Mtb to autophagosomes within infected cells is ineffective at controlling replication in vivo. We discovered that autophagy-associated proteins function outside xenophagy impact pathogenesis. reveal distinct autophagy-independent FIP200 CD11c+ alveolar macrophages (AMs) control burden mice lungs 7 days post-infection. Intra-tracheal airway labelling shows despite...
Abstract Poly ADP ribose inhibitors (PARPi) are most effective in ovarian cancer tumors with homologous recombination (HR) deficiency. Our group has shown that histone deacetylase (HDACi) sensitize HR proficient cells to PARPi. current efforts directed towards understanding how this therapeutic regimen alters tumor-associated macrophages (TAMs) murine models of high grade serous cancer. To investigate the effects Ola combined Ent, we used HR-proficient ID8 P53 wild-type and P53−/− syngeneic...
Abstract Tuberculosis, caused by Mycobacterium tuberculosis (Mtb), is a leading health concern. The rise in antibacterial resistance has steered focus to developing host directed therapies, which requires an in-depth understanding of Mtb interactions with the immune system. Xenophagy, targeting autophagosomes within infected cells, not effective at controlling replication vivo, however, we have discovered that autophagy proteins function outside xenophagy impact pathogenesis. FIP200 pathway...
Abstract Irgm1 is a 47kDa IFN-γ inducible GTPase that has been shown to be essential for controlling Mycobacterium tuberculosis (Mtb) infection in mice. It was originally recruited Mtb containing phagosomes infected macrophages where it proposed involved autophagy-mediated clearance of Mtb. However, we have previously discovered autophagy not required control replication. In addition, since reported does colocalize with bovis BCG treated cells, suggesting must non-autophagic roles TB require...
Neutrophils are the most abundant cell type in airways of tuberculosis patients. Recent investigations reported induction neutrophil extracellular traps (NETs) during Mycobacterium (Mtb) infection, however, molecular regulation and impact NETosis on Mtb pathogenesis is unknown. We find that response to infection neutrophils, PAD4 citrullinates histones decondense chromatin gets packaged into vesicles for release as NETs a manner can maintain viability promote replication. Type I interferon,...
Sumanta K. Naik works in the tuberculosis field, with a specific interest host immune response to Mycobacterium infection. In this mSphere of Influence article, he reflects on how paper "IRGM1 links mitochondrial quality control autoimmunity" by Prashant Rai et al. (Nat Immunol, 22:312-321, 2021, https://doi.org/10.1038/s41590-020-00859-0) impacted his research revealing new roles for Irgm1 responses.