A5079672191- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Electrochemical Analysis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Ion-surface interactions and analysis
- Fuel Cells and Related Materials
- Analytical Chemistry and Sensors
- Microfluidic and Bio-sensing Technologies
- Electrostatics and Colloid Interactions
- Lipid Membrane Structure and Behavior
- Membrane-based Ion Separation Techniques
- Geophysical and Geoelectrical Methods
- RNA and protein synthesis mechanisms
- Electrochemical sensors and biosensors
- Electrocatalysts for Energy Conversion
- Gold and Silver Nanoparticles Synthesis and Applications
- Molecular Junctions and Nanostructures
- RNA Interference and Gene Delivery
- Ion channel regulation and function
- Conducting polymers and applications
- Advanced Electron Microscopy Techniques and Applications
- Advanced Chemical Sensor Technologies
- Mass Spectrometry Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
- Advanced battery technologies research
Nanjing University
2019-2025
Imaging Center
2025
Molecular Sensing (United States)
2025
Institute for Biomedicine
2024-2025
East China University of Science and Technology
2014-2024
State Key Laboratory of Analytical Chemistry for Life Science
2019-2022
Institute of Molecular Functional Materials
2017
Moscow State University of Fine Chemical Technologies
2016
Shanghai Institute of Materia Medica
2013
Academia Sinica
2013
Capturing real-time electron transfer, enzyme activity, molecular dynamics, and biochemical messengers in living cells is essential for understanding the signaling pathways cellular communications. However, there no generalizable method characterizing a broad range of redox-active species single cell at resolution compartments. Although nanoelectrodes have been applied intracellular detection species, fabrication to maximize signal-to-noise ratio probe remains challenging because stringent...
ADVERTISEMENT RETURN TO ISSUEReviewNEXTElectrochemical Sensing at a Confined SpaceSi-Min LuSi-Min LuState Key Laboratory of Analytical Chemistry for Life Science, School and Chemical Engineering, Nanjing University, 210023, P. R. ChinaMore by Si-Min LuView Biography, Yue-Yi PengYue-Yi PengState PengView Yi-Lun YingYi-Lun YingState YingView Biographyhttp://orcid.org/0000-0001-6217-256X, Yi-Tao Long*Yi-Tao LongState China*Email: [email protected]More LongView...
Single-molecule measurements have greatly enhanced our understanding of living systems. Biological systems offer nanopores, a sub-class membrane proteins, the well-defined confined space for accommodating single molecule. The biological nanopore acts as single-biomolecule interface capturing and identifying molecule interest, thus it can be used single-molecule sensor. In this Perspective, we focus on nanopore-based interfaces detection. First, outline design interface, which provides rich...
β-Amyloid 42 (Aβ42) is the predominant form of amyloid peptide, which found in plaques brains Alzheimer's (AD) patients and one most abundant components aggregates. Information Aβ42 aggregation states essential for developing an understanding pathologic process amyloidoses. Here, we used α-hemolysin (α-HL) pores to probe different transition presence β-cyclodextrin (β-CD), a promoter aggregations, Congo red (CR), inhibitor aggregations. Analyzing characteristic transit duration times...
We report experiments and simulations demonstrating that multiple distinct motion trajectories of individual nanoparticles can be discerned from time-resolved current traces.
Nanoelectrodes, with dimensions below 100 nm, have the advantages of high sensitivity and spatial resolution. These electrodes attracted increasing attention in various fields such as single cell analysis, single-molecule detection, particle characterization high-resolution imaging. The rapid growth novel nanoelectrodes nanoelectrochemical methods brings enormous new opportunities field. In this perspective, we discuss challenges, advances, for nanoelectrode fabrication, real-time...
Noble metal nanoparticles have excellent optical and chemical properties are widely used in optics, sensors, biomedicines. The inherent characteristics of nanoparticles, particularly their size, play important roles applications. ability to readily measure the size single nanomaterials on-site is crucial rapid development single-particle sensors. In this study, we developed a facile real-time method for estimating diameter gold (GNPs) that range from 35 110 nm diameter; technique uses...
Data analysis for nanopore experiments remains a fundamental and technological challenge because of the large data volume, presence unavoidable noise, filtering effect. Here, we present an accurate robust process that recognizes current blockades enables evaluation dwell time amplitude through novel second-order-differential-based calibration method integration method, respectively. We applied developed to analyze both generated blockages experimental data. Compared results obtained using...
Clarifying the hidden but intrinsic feature of single nanoparticles by nanoelectrochemistry could help understand its potential for diverse applications. The uncontrolled interface and bandwidth limitation in electrochemical measurement put obstacle particle collision. Here, we demonstrate a well-defined 30 nm nanopore electrode with rapid chemical-electrochemical fabrication method which provides high reproducibility both size performance. A capacitance-based detection mechanism is...
Changes in the nanopore ionic current during entry of a target molecule underlie sensing capability and dominate intensity extent applications approach. The volume exclusion model has been proposed corrected to describe blockage. However, increasing evidence shows nonconformity with this model, suggesting that within should be entirely reconsidered. Here, we revisit origin blockage from theoretical perspective propose noncovalent interactions between affect conductance solution inside...
Chirality is essential in nearly all biological organizations and chemical reactions but rarely considered due to technical limitations identifying L/D isomerization. Using OmpF, a membrane channel from Escherichia coli with an electrostatically asymmetric constriction zone, allows discriminating chiral amino acids single peptide. The heterogeneous distribution of charged residues OmpF causes strong lateral electrostatic field at the constriction. This laterally zone forces sidechains...
The droplet nanopore technique is developed for multichannel high-throughput and low-volume single biomarker detection at the sub-microliter level, effectively lowering limit biomarkers to below 100 pg in a 0.4 μL droplet.
Nanopore technology holds great potential for single-molecule identification. However, extracting meaningful features from ionic current signals and understanding the molecular mechanisms underlying specific remain unresolved. In this study, we uncovered a distinctive pattern in K238Q aerolysin nanopore, characterized by transient spikes superimposed on two stable transition states. By employing neural network model, demonstrated that these previously overlooked dynamic spike exhibit...
Glycans, unlike uniformly charged DNA and compositionally diverse peptides, are typically uncharged exhibit rich stereoisomeric diversity in the glycosidic bonds between two monosaccharide units. This heterogeneity of charge structural complexity present significant challenges for accurate analysis. Herein, we developed a novel single‐molecule oligosaccharide sensor, OmpF nanopore. The natural electroosmotic flow within provides robust driving force unlabeled neutral oligosaccharides,...