A5032266932- Nanopore and Nanochannel Transport Studies
- Ion-surface interactions and analysis
- Microfluidic and Capillary Electrophoresis Applications
- Advanced biosensing and bioanalysis techniques
- Fuel Cells and Related Materials
- Microfluidic and Bio-sensing Technologies
- RNA and protein synthesis mechanisms
- Membrane-based Ion Separation Techniques
- Electrochemical Analysis and Applications
- Machine Learning in Bioinformatics
- Molecular Communication and Nanonetworks
- Ion channel regulation and function
- Protein Structure and Dynamics
- Graphene research and applications
- DNA and Nucleic Acid Chemistry
- Chalcogenide Semiconductor Thin Films
- Carbon Nanotubes in Composites
- Lipid Membrane Structure and Behavior
- Mechanical and Optical Resonators
- Spectroscopy and Quantum Chemical Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Solid-state spectroscopy and crystallography
- Biosensors and Analytical Detection
- Perovskite Materials and Applications
- Molecular Junctions and Nanostructures
Nanjing University
2020-2025
Imaging Center
2025
Molecular Sensing (United States)
2025
East China University of Science and Technology
2016-2023
Changes in the nanopore ionic current during entry of a target molecule underlie sensing capability and dominate intensity extent applications approach. The volume exclusion model has been proposed corrected to describe blockage. However, increasing evidence shows nonconformity with this model, suggesting that within should be entirely reconsidered. Here, we revisit origin blockage from theoretical perspective propose noncovalent interactions between affect conductance solution inside...
Abstract Quantum biological tunnelling for electron transfer is involved in controlling essential functions life such as cellular respiration and homoeostasis. Understanding the quantum effects biology has potential to modulate functions. Here we merge wireless nano-electrochemical tools with cancer cells control over trigger cell death. Gold bipolar nanoelectrodes functionalized redox-active cytochrome c a redox mediator zinc porphyrin are developed electric-field-stimulating bio-actuators,...
Nanopore technology holds great potential for single-molecule identification. However, extracting meaningful features from ionic current signals and understanding the molecular mechanisms underlying specific remain unresolved. In this study, we uncovered a distinctive pattern in K238Q aerolysin nanopore, characterized by transient spikes superimposed on two stable transition states. By employing neural network model, demonstrated that these previously overlooked dynamic spike exhibit...
Here, we show a designed solid-state nanopore sensor for the direct sensing and quantification of prostate-specific antigen (PSA) as cancer biomarker in serum without any pretreatment. This technique provides convenient, fast, low-cost biomarkers clinical samples.
A solid-state nanopore based method is described for resolving protein-folding-related problems <italic>via</italic> snapshotting the folding intermediates and characterizing kinetics of a single peptide.
By developing lithium-ion-active aerolysin, for the first time we have achieved aerolysin detection of single-stranded DNA longer than 100 nt.
Herein, the unzipping and translocation of DNA duplexes through a sub-2 nm silicon nitride (SiNx) solid-state nanopore have been demonstrated by well-resolved three-level blockades. In order to examine our observations, we applied simple model which is applicable processes nanopores. The generation these highly recognizable signatures an important step towards real applications nanopores in complex samples.
Single protein sensing based on solid-state nanopores is promising but challenging, because the fast translocation velocity of a beyond bandwidth nanopore instruments. To decelerate speed, here, we employed common cross-link interaction to achieve general and robust platform for single-molecule detection protein. Benefiting from EDC/NHS coupling between proteins, 10-fold decrease in speed has been achieved. The clearly distinguishable current signatures further reveal that anisotropic...
The time-domain nanopore signal is preciously converted into energy–frequency–time spectra with high frequency resolution and time using the Hilbert–Huang transform for revealing detail behaviours of single-molecule weak interactions.
Solid-state nanochannels with probe modification demonstrate effective spontaneous charge modulation and selective ionic current regulation. Outer-surface functionalization of these enables tunable signals before after analyte detection. To modulate local distributions, we designed sensing significant ion rectification properties for protein In this work, utilized asymmetrically charged DNA modifications to generate abundant information multianalyte recognition. During detection, probes on...
Docking of a protein–DNA complex onto nanopore can provide ample observation time, and has enabled collection analytic applications biological nanopores, including DNA sequencing. However, the application same principle to solid-state nanopores is tempered by poor understanding docking process. Here, we elucidate behavior individual complexes docked monitoring ionic current. Repeat monovalent streptavidin–DNA found produce current blockades that fluctuate between discrete levels. We roles...
The precise transportation of fluorescent probes to the designated location in living cells is still a challenge. Here, we present new addition nanopipettes as powerful tool deliver molecules given place single cell by electroosmotic flow, indicating favorable potential for further application single-cell imaging.
Direct protein analysis from complex cellular samples is crucial for understanding diversity and disease mechanisms. Here, we explored the potential of SiN x solid-state nanopores single-molecule samples. Using LOV2 as a model, designed nanopore electrophoretic driver fused it with LOV2, thereby enhancing capture efficiency target protein. Then, performed ex situ single-cell by directly extracting contents individual cells using glass nanopipette-based extraction successfully identified...
Herein, the structural stability of single azobenzene modified DNA duplexes, including trans form and cis form, has been examined separately based on their distinguishable unzipping kinetics from mixture by an α-hemolysin nanopore. Furthermore, accurate isomerization efficiency between was obtained with molecule resolution.
We developed a bipolar SiNx nanopore for the observation of single-molecule heterogeneous enzymatic dynamics. Single glucose oxidase was immobilized inside and its electrocatalytic behaviour real-time monitored via continuous recording ionic flux amplification. The temporal heterogeneity in properties spatial dynamic orientations were observed simultaneously, these two found to be closely correlated. anticipate that this method offers new perspectives on correlation protein structure...
A hybrid light/ToF-SIMS system was used to analyze the dynamic chemical changes of perovskite CH3NH3PbI3 films under light illumination, in order reveal mechanism instability for materials. Real-time material degradation and quasi-reversible iodine migration were successfully observed.
Nanopore measurement has advanced in single-molecule analysis by providing a transient time and confined space window that only allows one interested molecule to exist. By optimization integration of the electrical optical strategies this window, acquisition comprehensive information could be achieved resolve intrinsic properties heterogeneity single molecule. In work, we present roadmap build unified electrochemical synchronous platform for research We design low-cost ultralow-current...
The temperature effects on nanobubble nucleation have been analyzed with a SiN<sub>X</sub>solid-state nanopore.
Millions of years evolution have produced membrane protein channels capable efficiently moving ions across the cell membrane. The underlying fundamental mechanisms that facilitate these actions greatly contribute to weak non-covalent interactions. However, uncovering dynamic interactions and its synergic network effects still remains challenging in both experimental techniques molecule dynamics (MD) simulations. Here, we present a rational strategy combines MD simulations frequency-energy...
A fundamental question in peptide folding/unfolding is how the fleets through a set of transition states which dominate dynamics biomolecular folding path. Owing to their rapid duration and sub-nm structure difference, however, they have always been oversimplified because limited instrumental resolution. 1-3 Moreover, most experiments indicate single fold pathway while simulations suggest peptides owns preference multiple pathways. Using electrochemical confined effect solid-state nanopore,...
Solid-state nanopore has been widely used for a variety of single-molecule analysis due to its advantage size control, high stability, adjustable surface properties, and the potential further integration.Therefore, insensitive studies have applied DNA, RNA proteins.In order detect an oligonucleotide at level, here, we fabricated two SiNX solid-state nanopores with diameter 3.1 8.5 nm by controlled dielectric breakdown.The weak interaction between could be efficiently enhanced electrochemical...
Millions of years evolution have produced membrane protein channels capable efficiently moving ions across the cell membrane. The underlying fundamental mechanisms that facilitate these actions greatly contribute to weak non-covalent interactions. However, uncovering dynamic interactions and its synergic network effects still remains challenging in both experimental techniques molecule dynamics (MD) simulations. Here, we present a rational strategy combines MD simulations frequency-energy...