A5055775171- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Polyamine Metabolism and Applications
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Amino Acid Enzymes and Metabolism
- DNA Repair Mechanisms
- Cancer-related gene regulation
- PI3K/AKT/mTOR signaling in cancer
- SARS-CoV-2 detection and testing
- Light effects on plants
- Biochemical and Molecular Research
- Photoreceptor and optogenetics research
- Mast cells and histamine
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- Photosynthetic Processes and Mechanisms
- Microbial Natural Products and Biosynthesis
- Peptidase Inhibition and Analysis
- Cystic Fibrosis Research Advances
- Bacterial biofilms and quorum sensing
Marche Polytechnic University
2015-2024
University of Palermo
2023
Università di Camerino
1989-2014
Ospedali Riuniti di Ancona
1996-2007
University of Calabria
1988-2001
Délégation Paris 6
1998
Aberystwyth University
1998
Heidelberg University
1998
Max Planck Institute for Molecular Genetics
1989-1993
Max Planck Society
1989-1992
The hns (27 min) gene encoding the 15.4-kDa nucleoid protein H-NS was shown to belong cold shock regulon of Escherichia coli, its expression being enhanced 3- 4-fold during growth lag that follows a shift from 37 degrees C 10 C. A 110-base-pair (bp) DNA fragment containing promoter fused promoterless cat (hns-cat fusion) conferred similar response chloramphenicol acetyltransferase (CAT) activity in vivo and coupled transcription-translation systems prepared with extracts cold-shocked cells....
Translational initiation factor 2 (IF2) is a guanine nucleotide-binding protein that can bind guanosine 3′,5′-(bis) diphosphate (ppGpp), an alarmone involved in stringent response bacteria. In cells growing under optimal conditions, the GTP concentration very high, and of ppGpp low. However, stress may decline by as much 50%, attain levels comparable to those GTP. Here we show IF2 binds at same site with similar affinity Thus, be considered two alternative physiologically relevant ligands....
Summary The primary sequence of H‐NS (136 amino acid residues, Mr = 15402), an abundant Escherichia coli DNA‐binding protein, has been elucidated and its quaternary structure investigated by protein‐protein cross‐linking reactions. It was found that exists predominantly as a dimer, even at very low concentrations, but may form tetramers higher concentrations the interaction responsible for dimerization is chiefly hydrophobic.
Gel shift and DNase I footprinting experiments showed that Escherichia coli FIS (factor for inversion stimulation) protein binds to at least seven sites in the promoter region of hns . These extend from −282 +25 with two sites, closely flanking DNA bend located −150 transcriptional startpoint, partly overlapping H‐NS binding involved autorepression The interplay between FIS, were studied by examining effects on vitro transcription hns–cat fusions, as well looking effect preformed complexes...
The influence of the rare initiation triplet AUU on mRNA translation was investigated by comparing activity two pairs model mRNAs that differ in length Shine-Dalgarno and spacer sequences. Irrespective (AUG or AUU), all had similar template vitro, but depended more factor (IF) 2 less IF3 than AUG mRNAs. Increasing IF3/ribosome ratio from to 10 progressively inhibited abolished their capacity compete for translating ribosomes with other did not affect effects induced are its different on-...
Reliable point-of-care (POC) rapid tests are crucial to detect infection and contain the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The emergence several variants concern (VOC) can reduce binding affinity diagnostic antibodies, limiting efficacy currently adopted tests, while showing unaltered or increased for host receptor, angiotensin converting enzyme (ACE2). We present a graphene field-effect transistor (gFET) biosensor design, which exploits Spike-ACE2...
During initiation of protein synthesis in bacteria, translation factor IF2 is responsible for the recognition initiator tRNA (fMet-tRNA). To perform this function, binds to ribosome interacting with both 30S and 50S ribosomal subunits. Here we report topographical localization on 70S determined by base-specific chemical probing. Our results indicate that specifically protects from modification two sites domain V 23S rRNA, namely A2476 A2478, residues around position 2660 VI, so-called...
Many known antibiotics target the translational apparatus, but none of them can selectively inhibit initiation protein synthesis and/or is prokaryotic-specific. This article describes properties GE81112, an effective and prokaryotic-specific inhibitor. GE81112 a natural tetrapeptide produced by Streptomyces sp. identified in vitro high-throughput screening test developed to find inhibitors prokaryotic apparatus preferentially acting on steps other than elongation. In vivo inhibits not cell...
By means of limited proteolysis Bacillus stearothermophilus initiation factor IF2 and genetic manipulation its structural gene, infB, we have been able to produce (or hyperproduce) purify two polypeptide fragments corresponding structurally functionally separate domains the protein. The first is G-domain (approximately 41 kDa), which makes up central part molecule contains conserved elements found in all GTP/GDP-binding sites G-proteins. This domain resistant presence GTP or GDP, retains...
Eukaryotic Initiation factor 6 (eIF6) is a peculiar translation initiation that binds to the large 60S ribosomal subunits, controlling and participating in ribosome biogenesis. In past, knowledge about mechanisms adopted by cells for protein synthesis extracellular stimuli has focused on two factors (eIF4E eIF2), however, recent data suggest eIF6 as newcomer control of downstream signal transduction pathways. over-expressed tumors its decreased expression renders less prone tumor growth. A...
Abstract Field‐effect transistor (FET)‐based sensors are increasingly gaining relevance in diagnostic, healthcare, and environmental monitoring applications. A FET operates by transducing chemical interactions between a surface‐immobilized bioreceptor the target analyte into detectable electrical signal. biosensors can detect monitor molecules (i.e., biomarkers, small molecules, viruses, bacterias) present liquid samples, making “liquid gate” configuration of FETs most suitable approach....
The translation factor IF6 is shared by the Archaea and Eukarya, but not found in Bacteria. properties of eukaryal (eIF6) have been extensively studied, remain somewhat elusive. eIF6 behaves as a ribosome-anti-association involved miRNA-mediated gene silencing; however, it also seems to participate ribosome synthesis export. Here we determined function ribosomal localization archaeal (Sulfolobus solfataricus) homologue (aIF6). We find that aIF6 binds specifically 50S subunits, hindering...
The downstream box (DB) has been proposed to enhance translation of several mRNAs and be a key element controlling the expression cold-shocked mRNAs. However, proposal that DB exerts its effects through base pairing interaction with complementary anti-downstream (antiDB) sequence (nt 1469–1483) located in penultimate stem (helix 44) 16S rRNA remains controversial. existence this during initiation protein synthesis under cold-shock conditions investigated present work using an Escherichia...
MBF1 (multi-protein bridging factor 1) is a protein containing conserved HTH (helix-turn-helix) domain in both eukaryotes and archaea. Eukaryotic has been reported to function as transcriptional co-activator that physically bridges transcription regulators with the core initiation machinery of RNA polymerase II. In addition, found be associated polyadenylated mRNA yeast well mammalian cells. aMBF1 (archaeal MBF1) very among most archaeal lineages; however, its so far remained elusive. To...