A5042980294- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Microbial Community Ecology and Physiology
- Microplastics and Plastic Pollution
- Environmental DNA in Biodiversity Studies
- DNA and Nucleic Acid Chemistry
- Pharmaceutical and Antibiotic Environmental Impacts
- Diatoms and Algae Research
- Identification and Quantification in Food
- CRISPR and Genetic Engineering
- Antimicrobial agents and applications
- Marine Toxins and Detection Methods
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- interferon and immune responses
- Microbial Natural Products and Biosynthesis
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Probiotics and Fermented Foods
- Graphene and Nanomaterials Applications
- Chemical Synthesis and Analysis
Università di Camerino
2015-2025
MRC Laboratory of Molecular Biology
2011-2012
Ospedali Riuniti di Ancona
2001
University of Calabria
2001
Max Planck Institute for Molecular Genetics
1989-1991
The hns (27 min) gene encoding the 15.4-kDa nucleoid protein H-NS was shown to belong cold shock regulon of Escherichia coli, its expression being enhanced 3- 4-fold during growth lag that follows a shift from 37 degrees C 10 C. A 110-base-pair (bp) DNA fragment containing promoter fused promoterless cat (hns-cat fusion) conferred similar response chloramphenicol acetyltransferase (CAT) activity in vivo and coupled transcription-translation systems prepared with extracts cold-shocked cells....
Expression of a promoterless cat gene fused to DNA fragment approximately 400 bp, beginning at -313 Escherichia coli hns, was significantly repressed in E. and Salmonella typhimurium strains with wild-type hns but not mutants carrying alleles. CAT expression from fusions containing shorter (110 bp) segment essentially unaffected the same genetic backgrounds. The stage growth found influence extent repression which maximum (approximately 75%) mid-log cultures negligible cells entering...
Abstract Cell-free protein synthesis (CFPS) systems offer a versatile platform for wide range of applications. However, the traditional methods detecting proteins synthesized in CFPS, such as radioactive labeling, fluorescent tagging, or electrophoretic separation, may be impractical, due to environmental hazards, high costs, technical complexity, and time consuming procedures. These limitations underscore need new approaches that streamline detection process, facilitating broader...
Current knowledge about the spread of pathogens in aquatic environments is scarce probably because bacteria, viruses, algae and their toxins tend to occur at low concentrations water, making them very difficult measure directly. The purpose this study was development validation tools detect freshwater systems close an urban area. In order evaluate anthropogenic impacts on water microbiological quality, a phylogenetic microarray developed context EU project µAQUA simultaneously numerous...
By means of limited proteolysis Bacillus stearothermophilus initiation factor IF2 and genetic manipulation its structural gene, infB, we have been able to produce (or hyperproduce) purify two polypeptide fragments corresponding structurally functionally separate domains the protein. The first is G-domain (approximately 41 kDa), which makes up central part molecule contains conserved elements found in all GTP/GDP-binding sites G-proteins. This domain resistant presence GTP or GDP, retains...
Around the globe, escalation in rare opportunistic microbial infections is alarming as they are heading steadily towards 'superbug' status. In aquatic ecosystems, plastic fosters multidrug-resistant pathogenic bacteria and plays a significant role trafficking antibiotic-resistant genes. this study, we focused on bacterial strain isolated from communities found substrates of volcanic lake central Italy.Extended-spectrum beta-lactamase-producing strains were both raw water for comparative...
Antibiotic-resistant bacteria (ARB) adhesion onto plastic substrates is a potential threat to environmental and human health. This current research investigates the prevalence of two relevant pathogens, Staphylococcus spp. Klebsiella spp., their sophisticated equipment antibiotic-resistant genes (ARGs), retrieved from submerged into an inland water body. The results microbiological analysis on selective chromogenic media revealed presence colonies with distinctive phenotypes, which were...
<title>Abstract</title> Ribosome assembly is a multistep process that ensures functional ribosome structure. The molecular mechanism ribosomeassociated GTPases (RAGTPases) use to enhance accuracy, remains largely be elucidated. Here, we systematic evolution of ligands by exponential enrichment (SELEX), followed sequencing, comprehensive bioinformatics analysis, and biochemical characterization identify aptamers target the RA-GTPase ERA <italic>Staphylococcus aureus</italic>. ELONA...
The molecular determinants necessary and sufficient for recognition of its specific DNA target are contained in the C-terminal domain (H-NSctd) nucleoid-associated protein H-NS. H-NSctd protects from DNaseI cleavage a few short segments H-NS-sensitive hns promoter whose sequences closely match recently identified H-NS consensus motif (tCG(t/a)T(a/t)AATT) and, alone or fused to oligomerization phage lambda CI repressor, inhibits transcription vitro vivo. importance is indicated by fact that...
A few diatom species produce toxins that affect human and animal health. Among these, members of the Pseudo-nitzschia genus were first diatoms unambiguously identified as producer domoic acid, a neurotoxin affecting molluscan shell-fish, birds, marine mammals, humans. Evidence exists indicating involvement another genus, Amphora, potential acid. We present strategy for detection Amphora coffeaeformis based on development species-specific oligonucleotide probes their application in microarray...
Monitoring the quality of freshwater is an important issue for public health. In context European project μAqua, 150 samples were collected from several waters in France, Germany, Ireland, Italy, and Turkey 2 yr. These analyzed using multitoxin detection methods previously developed: a microsphere-based method coupled to flow-cytometry, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. The presence microcystins, nodularin, domoic acid, cylindrospermopsin,...
ABSTRACT A chemical derivative of the thiopeptide GE2270A, designated NAI003, was found to possess a substantially reduced antibacterial spectrum in comparison parent compound, being active against just few Gram-positive bacteria. In particular, NAI003 retained low MICs all tested isolates Propionibacterium acnes and, lesser extent, Enterococcus faecalis . Furthermore, showed time- and dose-dependent killing both clindamycin-resistant clindamycin-sensitive P. isolate. Gel shift experiments...