A5076608807- Environmental DNA in Biodiversity Studies
- Marine and coastal plant biology
- Marine Bivalve and Aquaculture Studies
- Identification and Quantification in Food
- Marine Biology and Ecology Research
- Aquatic Invertebrate Ecology and Behavior
- Advanced biosensing and bioanalysis techniques
- Air Quality Monitoring and Forecasting
- Microbial Community Ecology and Physiology
- Coral and Marine Ecosystems Studies
- Effects and risks of endocrine disrupting chemicals
- Pharmaceutical and Antibiotic Environmental Impacts
- Genetic diversity and population structure
- Marine and fisheries research
- Microplastics and Plastic Pollution
- SARS-CoV-2 detection and testing
- Advanced Chemical Sensor Technologies
- Fish Ecology and Management Studies
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Water Treatment and Disinfection
- Antimicrobial agents and applications
- Plant Disease Resistance and Genetics
- Aquaculture Nutrition and Growth
- Cephalopods and Marine Biology
Norwegian Institute for Water Research
2015-2025
University of Louisiana at Lafayette
2021
University of Oslo
2000-2018
Nel (Norway)
2007
HAL is a
Endocrine-disrupting contaminants have been associated with aberrant changes in epigenetic pathways animals. In this study, zebrafish embryos were exposed bisphenol A (BPA) to search for associations between behavior and mechanisms fish. For concentration-dependent responses, a range of BPA concentrations (0.1 nM 30 μM). Embryos analyzed locomotor activity at 3-, 4-, 5-days post fertilization (dpf) response changing light conditions. Based on effects gene expression, 10 μM [from 24 96 hours...
The removal of cell free DNA (plasmids) carrying antibiotic resistance genes (ARGs) was investigated at bench-scale using ultrafiltration (UF), nanofiltration (NF) and reverse osmosis (RO) membranes commonly applied in water reuse applications. the plasmid spiked to ultrapure determined a direct qPCR method. More than 99% achieved by with 1 kDa molecular weight cut off (MWCO). Membranes lower MWCO showed complete under specific experimental conditions, reaching maximum log reduction value...
The Pacific oyster, Crassostrea gigas, was introduced to Europe for aquaculture purposes, and has had a rapid unforeseen northward expansion in northern Europe. recent dramatic increase number of C. gigas populations along the species' distribution limit questioned efficiency Skagerrak as dispersal barrier transport survival larvae. We investigated genetic connectivity possible spreading patterns between oyster on southern Norwegian coast (4 localities) Swedish Danish by means DNA...
A new label-free electrochemical DNA (E-DNA) biosensor using a custom synthesized ferrocenyl (Fc) double-stranded intercalator as redox marker is presented. Single-stranded (ssDNA) was co-immobilized on gold electrodes with 6-mecarpto-hexanol to control the surface density of ssDNA probe, and hybridized complementary DNA. The binding Fc dsDNA measured by differential pulse voltammetry. This optimized allow detection single base pair mismatched sequences, able detect low 10 pM target dynamic...
The ability to detect founding populations of invasive species or rare with low number individuals is important for aquatic ecosystem management. Traditional approaches use historical data, knowledge the species' ecology and time-consuming surveys. Within past decade, environmental DNA (eDNA) has emerged as a powerful additional tracking tool. While much work been done animals, comparatively very little plants. Here we investigated transportation seasonal changes in eDNA concentrations an...
Coralline red algae in the non-geniculate genera Clathromorphum, Phymatolithon and Lithothamnion are important benthic ecosystem engineers photic zone of Arctic Subarctic. In these regions, systematics biogeography Clathromorphum have mostly been resolved whereas has not, until now. Seventy-three specific infraspecific names were given to Subarctic specimens late 19th early 20th century by Frans R. Kjellman Mikael H. Foslie. DNA sequences from 36 type specimens, five historical an extensive...
ABSTRACT Kelp forests are dynamic coastal habitats that generate large amounts of carbon‐rich detritus. The fate this detritus is largely unknown and considered a missing link in global carbon budgets. can serve as food for benthic invertebrates pelagic invertebrate larvae, but we know close to nothing about the role kelp other zooplankton. We conducted feeding experiments test if highly abundant copepod Calanus finmarchicus feed on fragments two dominant species, Saccharina latissima...
The distributional patterns of Arctic species are commonly affected by the recurring Pleistocene glaciations, which contributed to transient or permanent genetic isolation. Here we explore phylogeography climate-sensitive fairy shrimp Branchinecta paludosa, has a circumpolar range distribution, with certain southern alpine outreaches. We sequenced mitochondrial cytochrome oxidase I subunit from samples collected at ten Nearctic and nine Palaearctic sites, including populations. A handful...
We compared the genetic differentiation in green sea urchin Strongylocentrotus droebachiensis from discrete populations on NE Atlantic coast. By using eight recently developed microsatellite markers, structure was between Danish Strait south to Barents Sea north (56-79°N). Urchins are spread by pelagic larvae and may be transported long distances northwards-going ocean currents. Two main superimposed patterns were identified. The first showed a subtle but significant southernmost...
A dose-response study on a plasmid vector containing ampicillin and kanamycin resistance genes was performed with UVC-LEDs at two different wavelengths (265 285 nm) using UV fluences including 40 mJ/cm2 186 cm2, typically associated 4-log inactivation of bacterial pathogens viruses, respectively. DNA damage assessed qPCR transformation assays: four protocols targeting the were designed to produce amplicons varying in lengths from 80 601 bp quantify damage. irradiation emitted 265 nm more...
Early molecular events with correlation to disease, such as aberrant DNA methylation, emphasize the importance of methylation a potential environmental biomarker. Currently, little is known regarding how various contaminants and mixtures alter in aquatic organisms, testing both time- labor-consuming. Therefore, an vitro screening method was evaluated by exposing zebrafish liver cells (ZF-L) for 96 h nonmutagenic model substance 5′-azacytidine (AZA), well selection pollutants sodium arsenite...
This study presents an improved duplex quantitative polymerase chain reaction ( qPCR ) method using the master sex‐determining gene sdY as a marker for simultaneous genetic sex identification of salmonids Salmo genus and paternity elucidation salar × trutta hybrids. will provide new, simple economical molecular tool ecological studies these species well aquaculture purposes.
A number of epigenetic modulating chemicals are known to affect multiple generations a population from single ancestral exposure, thus posing transgenerational hazards. The present study aimed establish high-throughput (HT) analytical workflow for cost-efficient concentration-response analysis and phenotypic effects, support the development novel Adverse Outcome Pathway (AOP) networks DNA methyltransferase (DNMT) inhibitor-mediated effects on aquatic organisms. model DNMT inhibitor...
ABSTRACT An early warning system using a rapid enzymatic semiautomated method suitable for fecal coliform detection in recreational waters within 8 h was developed further and evaluated this study. This compared to the standard followed United Kingdom. We used 1,011 samples originating from 206 different locations Wales. When we assessed presence or absence of coliforms, targeting very low levels contamination, obtained 83.9% agreement between lauryl sulfate broth-membrane filtration...
The aetiology of several human diarrhoeas has been increasingly associated with the presence virulence factors rather than bacterial species hosting genes, exemplified by sporadic emergence new hosts. Two important are Shiga toxin (Stx) and E. coli outer membrane protein (Eae) or intimin, encoded stx eae respectively. Although polymerase chain reaction (PCR) protocols target these few aim at detecting all variants have an internal amplification control (IAC) included in a multiplex assay....
Abstract Coralline algae form extensive maerl and rhodolith habitats that support a rich biodiversity. Calcium carbonate harvesting as well trawling activities threatens this ecosystem. Eleven species were recorded so far maerl-forming in NE Atlantic, but identification based on morphological characters is unreliable. As for most red algae, we now use molecular to resolve of these taxa. However, obtaining DNA sequences requires time resource demanding methods. The purpose our study was...
Abstract Environmental DNA (eDNA) from water samples is increasingly used to detect the presence and distribution of species in aquatic ecosystems. However, before implementing eDNA monitoring programmes, various species‐specific sampling or analytical issues remain be resolved order minimize frequencies false‐positive ‐negative results. For example, empty shells freshwater pearl mussels ( Margaritifera margaritifera ) contain extractable (chemical extraction ground‐up shells) suggesting a...
The green sea urchin Strongylocentrotus droebachiensis has a wide circumpolar distribution and plays key role in coastal ecosystems worldwide by destructively grazing macroalgae beds turn them into marine deserts, so-called barren grounds. In the past decades, large established kelp forests have been overgrazed transformed to such grounds on Norwegian coast. This important repercussions for diversity production, including reproduction of several fish species relying as nurseries. Genetic is...
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Avoiding complementarity between primers when designing a PCR assay constitutes central rule strongly anchored in the mind of molecular scientist. 3’-complementarity will extend during elongation using one another as template, consequently disabling further possible involvement traditional target amplification. However, 5’-complementarity leave unchanged cycles, albeit sequestered to another, therefore also suppressing We show that may be exploited new method called...