A5015170140- Advanced Radiotherapy Techniques
- Lung Cancer Diagnosis and Treatment
- Spectroscopy Techniques in Biomedical and Chemical Research
- Management of metastatic bone disease
- Radiopharmaceutical Chemistry and Applications
- Brain Metastases and Treatment
- Spectroscopy and Chemometric Analyses
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Lung Cancer Treatments and Mutations
- Bee Products Chemical Analysis
- Network Security and Intrusion Detection
- Blind Source Separation Techniques
- Optical Imaging and Spectroscopy Techniques
- Medical Imaging and Pathology Studies
- Glioma Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- Hepatocellular Carcinoma Treatment and Prognosis
- Metabolomics and Mass Spectrometry Studies
- Cancer Genomics and Diagnostics
- Distributed and Parallel Computing Systems
- Advanced Image Processing Techniques
- Cybercrime and Law Enforcement Studies
- Statistical and numerical algorithms
- thermodynamics and calorimetric analyses
University of British Columbia
2014-2025
Positive Living North
2018-2025
North Carolina State University
2024
University of Toronto
2020-2024
BC Cancer Agency
2010-2023
John Wiley & Sons (United States)
2020-2023
University of Northern British Columbia
2022
Hudson Institute
2020
University of Victoria
2006-2014
After the publication of landmark SABR-COMET trial, concerns arose regarding high-grade toxic effects treatment with stereotactic ablative body radiotherapy (SABR) for oligometastases.To document SABR in a large cohort from population-based, provincial cancer program.From November 2016 to July 2020, 381 patients across all 6 centers British Columbia were treated this single-arm, phase 2 trial oligometastatic or oligoprogressive disease. During period, only eligible receive these settings...
In this work we investigate the capability of Raman microscopy (RM) to detect inherent sources biochemically based spectral variability between single cells a human tumor cell line (DU145) cultured in vitro. Principal component analysis (PCA) is used identify differences single-cell spectra. These correlate with (1) cycle progression and (2) changing confluency culture during first 3 4 days after sub-culturing. Cell regulatory drugs are synchronize cultures, flow cytometry determine...
This work applies noninvasive single-cell Raman spectroscopy (RS) and principal component analysis (PCA) to analyze correlate radiation-induced biochemical changes in a panel of human tumour cell lines that vary by tissue origin, p53 status intrinsic radiosensitivity. Six lines, derived from prostate (DU145, PC3 LNCaP), breast (MDA-MB-231 MCF7) lung (H460), were irradiated vitro with single fractions (15, 30 or 50 Gy) 6 MV photons. Remaining live cells harvested for RS at 0, 24, 48 72 h...
The drive toward personalized radiation therapy (RT) has created significant interest in determining patient-specific tumor and normal tissue responses to radiation. Raman spectroscopy (RS) is a non-invasive label-free technique that can detect response through assessment of radiation-induced biochemical changes cells. In the current study, single-cell RS identified specific four human epithelial cell lines: lung (H460), breast (MCF-7, MDA-MB-231), prostate (LNCaP), following exposure...
This work investigates the capability of Raman spectroscopy (RS) to study effects ionizing radiation on single human tumour cells. Prostate cells (cell line DU145) are cultured in vitro and irradiated doses between 15 50 Gy with fractions 6 MV photons. Single-cell spectra acquired from unirradiated cultures up 5 days post-irradiation. Principal component analysis is used distinguish uniquely radiation-induced spectral changes inherent sources variability arising cell cycle differences other...
Abstract This work combines single cell Raman spectroscopy (RS) with group and basis restricted non-negative matrix factorisation (GBR-NMF) to identify individual biochemical changes associated radiation exposure in three human cancer lines. The lines analysed were derived from lung (H460), breast (MCF7) prostate (LNCaP) tissue are known display varying degrees of radio sensitivity due the inherent properties each type. GBR-NMF approach involves deconstruction spectra into component bases...
Altered cellular metabolism is a hallmark of tumor cells and contributes to host properties associated with resistance radiotherapy. Detection radiation-induced biochemical changes can reveal unique metabolic pathways affecting radiosensitivity that may serve as attractive therapeutic targets. Using clinically relevant doses radiation, we performed label-free single cell Raman spectroscopy on series human cancer lines detected accumulation intracellular glycogen. The increase in glycogen...
Objectives We aimed to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform for the rapid detection of CHIKV in both patient and mosquito samples from Brazil. Methods optimized an RT-LAMP assay, then evaluated sensitivity specificity using visual detection. In comparison with RT-qPCR reference method, we validated utility this assay as molecular diagnostic test laboratory arbovirus diagnostics 100 serum collected suspected cases. Results Our specifically...
This study presents a new method of image signal-to-noise ratio (SNR) enhancement by utilizing newly developed 2D two-point maximum entropy regularization (TPMEM). When utilized as an filter, it is shown that TPMEM offers unsurpassed flexibility in its ability to balance the complementary requirements smoothness and fidelity. The technique evaluated for use x-ray computed tomography (CT) images irradiated polymer gels used radiation dosimetry. We utilize range statistical parameters (e.g....
Oligometastases refer to a state of disease where cancer has spread beyond the primary site, but is not yet widely metastatic, often defined as 1–3 or 1–5 metastases in number. Stereotactic ablative radiotherapy (SABR) an emerging technique treat oligometastases that require further prospective population-based toxicity estimates. This non-randomized phase II trial all participants will receive experimental SABR treatment sites newly diagnosed progressing oligometastatic disease. We accrue...
The global spread of SARS-CoV-2 has shaken our health care and economic systems, prompting re-evaluation long-held views on how best to deliver care. This is especially the case for diagnostic strategy. While current laboratory-based centralized RT-qPCR will continue serve as a gold standard into foreseeable future, shortcomings dependence this method have been laid bare. It now clear that robust diagnostics pandemic response strategy, like any disaster planning, must include adaptive,...
Abstract Background Bone metastases in the lower spine and pelvis are effectively palliated with radiotherapy (RT), though this can come side effects such as radiation induced nausea vomiting (RINV). We hypothesize that high rates of RINV occur part because widespread use inexpensive simple unplanned palliative (SUPR), over more complex resource intensive 3D conformal RT, volumetric modulated arc therapy (VMAT). Methods This is a randomized, multi-centre phase III trial SUPR versus VMAT....
Defending from cyberattacks requires practitioners to operate on high-level adversary behavior. Cyberthreat intelligence (CTI) reports past cyberattack incidents describe the chain of malicious actions with respect time. To avoid repeating incidents, must proactively identify and defend against recurring - which we refer as temporal attack patterns. Automatically mining patterns among provides structured actionable information behavior cyberattacks. The goal this paper is aid security in...
Abstract Background Radiotherapy delivery regimens can vary between a single fraction (SF) and multiple fractions (MF) given daily for up to several weeks depending on the location of cancer or metastases. With limited evidence comparing fractionation oligometastases, there is support explore toxicity levels nearby organs at risk as primary outcome while using SF MF stereotactic ablative radiotherapy (SABR) well differences in patient-reported quality life experience. Methods This study will...