A convenient, Agrobacterium-mediated transient expression assay has been evaluated for rapid analysis of plant promoters and transcription factors in vivo. By simple infiltration Agrobacterium cells carrying appropriate plasmid constructs into tobacco leaves planta, reproducible assays could be conducted as little 2-3 days without using expensive equipment (e.g. biolistic gun or electroporation apparatus) complicated procedures preparation protoplasts). Biotic abiotic treatments applied to...

Small RNAs (sRNA), including microRNAs (miRNA) and small interfering (siRNA), are produced abundantly in plants animals function regulating gene expression or defense against virus viroid infection. Analysis of siRNA profiles upon infection plant may allow for identification, strain differentiation, de novo assembly genomes. In the present study, four suspected virus-infected tomato samples collected U.S. Mexico were used sRNA library construction deep sequencing. Each generated between 5–7...

The complete genomic sequence of a new tobamovirus in tomatoes was determined through deep sequencing and assembly small RNAs, then validated Sanger sequencing. Based on the low identity (≤85%) to known viruses close phylogenetic relationship tobamoviruses, it identified as species.

In recent years, a number of serious disease outbreaks caused by viruses and viroids on greenhouse tomatoes in North America have resulted significant economic losses to growers. The objectives this study were evaluate the effectiveness commercial disinfectants against mechanical transmission these pathogens, select with broad spectrum reactivity control general virus viroid diseases tomato production. A total 16 evaluated Pepino mosaic (PepMV), Potato spindle tuber (PSTVd), Tomato (ToMV),...

Tomato mottle mosaic virus (ToMMV) was first identified in 2013 as a novel tobamovirus infecting tomatoes Mexico. In just few years, ToMMV has been several countries around the world, including United States. present study, we characterized molecular, serological, and biological properties of developed species-specific RT-PCR to detect three tomato-infecting tobamoviruses: Tobacco (TMV), (ToMV), ToMMV. Previously, reported Florida New York. this made two new reports on occurrences California...

Fifty-two germplasm accessions of Chinese vegetable brassicas were analyzed using 112 random amplified polymorphic DNA (RAPD) markers. The array material examined spanned a wide range morphological, geographic, and genetic diversity, included 30 Brassica rapa L. (Chinese cabbage, pakchoi, turnip, broccoletto), 18 B. juncea (L.) Czern. (leaf, stem, root mustards), four oleracea ssp. alboglabra kale). RAPD markers unambiguously identified all 52 accessions. Nei-Li similarities computed used in...

Cucumber green mottle mosaic virus (CGMMV), an emerging tobamovirus, has caused serious disease outbreaks to cucurbit crops in several countries, including the United States. Although CGMMV is seed-borne, mechanism of its transmission from a contaminated seed germinating seedling still not fully understood, and most suitable health assay method been well established. To evaluate transmissibility, using highly watermelon seeds collected CGMMV-infected experimental plants, bioassays were...

Pepino mosaic, once an emerging disease a decade ago, has become endemic on greenhouse tomatoes worldwide in recent years. Three distinct genotypes of mosaic virus (PepMV), including EU, US1 and CH2 have been recognized. Our earlier study conducted 2006–2007 demonstrated predominant EU genotype Canada United States. The objective the present was to monitor dynamic PepMV genetic composition its current status North America. Through yearly monitoring efforts 2009–2012, we detected dramatic...

Small interfering RNA (siRNA) duplexes are short (usually 21 to 24 bp) double-stranded RNAs (dsRNAs) with several overhanging nucleotides at both 5'- and 3'-ends. It has been found that siRNA bind the RNA-induced silencing complex (RISC) cleave sense strands endonucleases. In this study, for first time, we detected induced by plant viruses on a large scale using next-generation sequencing (NGS) data. addition, used nucleotide (nt) 2 nt overhangs construct dataset future data mining. The...

The complete genome sequence of Southern tomato virus (STV), a double-stranded RNA that affects in China, was determined using small deep sequencing. This Chinese isolate shares 99% identity to other isolates from Mexico, France, Spain, and the United States. is first report STV infecting tomatoes Asia.

The complete genome sequence (6,423 nucleotides [nt]) of an emerging cucumber green mottle mosaic virus (CGMMV) isolate on in North America was determined through deep sequencing small (sRNA) and rapid amplification cDNA ends. shares 99% nucleotide identity with the Asian genotype but only 90% European genotype.

ABSTRACT The complete genome sequence of a Southern tomato virus (STV) isolate on plants in seed production field Bangladesh was obtained for the first time using next-generation sequencing. identified isolate, STV_BD-13, shares high degree identity (99%) with several known STV isolates worldwide.

The complete genome sequence of an isolate tomato mottle mosaic virus (ToMMV) infecting tomatoes in New York was obtained using small RNA (sRNA) deep sequencing. ToMMV_NY-13 shared 99% identity with isolates from Mexico and Florida. Broader distribution this emerging is a cause for concern to the industry.

The complete genome sequence of a new isolate squash mosaic virus (SqMV) infecting plants in Spain was obtained using deep sequencing small RNAs. low nucleotide identities, with only 87 to 88% for RNA1 and 84 86% RNA2 known SqMV isolates, suggested that this belongs novel genotype.

Tomato brown rugose fruit virus (ToBRFV) was first described infecting tomatoes in 2015 after breaking tobamovirus resistance production cultivars. As an emerging pathogen, ToBRFV commercial assays were developed and subsequently validated. Agdia commercialized three assays: ELISA, ImmunoStrip, AmplifyRP XRT. The ELISA is a serological method utilizing monoclonal antibodies designed for use as high-throughput laboratory tool. ImmunoStrip lateral flow device field-screening XRT molecular...

Because (i) origins and genealogies of vetiver grass have been poorly documented, (ii) morphological uniformity infrequent flowering precluded accurate identification selected clones, an investigation was undertaken to link molecular methods with rigorous biometric analysis discriminate accessions clones. Successful DNA profiling is highly dependent on the reproducibility assay. Our ability generate consistent results may a result genomic quality, oligonucleotide primer selection, (ill)...