A5017910335- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Plant Disease Management Techniques
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Advances in Cucurbitaceae Research
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Plant Pathogens and Resistance
- Insect symbiosis and bacterial influences
- Transgenic Plants and Applications
- Phytoplasmas and Hemiptera pathogens
- Chromosomal and Genetic Variations
- Nematode management and characterization studies
- Genomics and Phylogenetic Studies
- Insect and Pesticide Research
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- CRISPR and Genetic Engineering
U.S. Vegetable Laboratory
2013-2024
Agricultural Research Service
2012-2021
Agriculture and Agri-Food Canada
2020
United States Department of Agriculture
2007-2016
Cornell University
1991-2016
Agricultural Research Organization
2016
U.S. Horticultural Research Laboratory
2014
Beijing Academy of Agricultural and Forestry Sciences
2011
North Central College
2011
Iowa State University
2011
The Cucurbitaceae family (cucurbit) includes several economically important crops, such as melon, cucumber, watermelon, pumpkin, squash and gourds. During the past years, genomic genetic data have been rapidly accumulated for cucurbits. To store, mine, analyze, integrate disseminate these large-scale datasets to provide a central portal cucurbit research breeding community, we developed Cucurbit Genomics Database (CuGenDB; http://cucurbitgenomics.org) using Tripal toolkit. database currently...
Genomic decay is a common feature of intracellular bacteria that have entered into symbiosis with plant sap-feeding insects. This study the whitefly Bemisia tabaci and two (Portiera aleyrodidarum Hamiltonella defensa) cohoused in each host cell investigated whether Portiera metabolism genes complemented by genes, compared metabolic traits other insects (aphids, psyllids, mealybugs). Parallel genomic transcriptomic analysis revealed genome contributes multiple reactions complement or...
Years of selection for desirable fruit quality traits in dessert watermelon (Citrullus lanatus) has resulted a narrow genetic base modern cultivars. Development novel genomic and resources offers great potential to expand diversity improve important watermelon. Here, we report high-quality genome sequence cultivar 'Charleston Gray', principal American watermelon, complement the existing reference from '97103', an East Asian cultivar. Comparative analyses between genomes Gray' '97103'...
Bottle gourd (Lagenaria siceraria) is an important vegetable crop as well a rootstock for other cucurbit crops. In this study, we report high-quality 313.4-Mb genome sequence of bottle inbred line, USVL1VR-Ls, with scaffold N50 8.7 Mb and the longest 19.0 Mb. About 98.3% assembled scaffolds are anchored to 11 pseudomolecules. Our comparative genomic analysis identifies chromosome-level syntenic relationships between cucurbits, lineage-specific gene family expansions in gourd. We...
Tomato spotted wilt tospovirus (TSWV), one of the most important plant viruses, causes yield losses to many crops including tomato. The current disease management for TSWV is based mainly on breeding tomato cultivars containing Sw-5 locus. Unfortunately, several resistance-breaking strains have been identified. Sw-7 an alternative locus conferring resistance a broad range strains. In effort uncover gene networks that are associated with resistance, we performed comparative transcriptome...
Abstract Sweetpotato ( Ipomoea batatas L.) is an important staple crop cultivated in over 100 countries, and the storage roots vines provide food for humans livestock. consumption demand its value‐added products have increased significantly last two decades led to new cultivar development, expansion acreage, United States export markets. Despite known nutritional components other health benefits, further research needed characterize genetic diversity chemical composition related their root...
To improve the simultaneous detection of two pathogens in cucurbit seed, a combination magnetic capture hybridization (MCH) and multiplex real-time polymerase chain reaction (PCR) was developed. Single-stranded DNA probes targeting Acidovorax avenae subsp. citrulli, causal agent bacterial fruit blotch, Didymella bryoniae, gummy stem blight, were covalently attached to particles used selectively concentrate template from seed samples. Sequestered DNAs subsequently amplified by PCR using...
The complete genomic sequence of a new tobamovirus in tomatoes was determined through deep sequencing and assembly small RNAs, then validated Sanger sequencing. Based on the low identity (≤85%) to known viruses close phylogenetic relationship tobamoviruses, it identified as species.
Whiteflies threaten agricultural crop production worldwide, are polyphagous in nature, and transmit hundreds of plant viruses. Little is known how whitefly gene expression altered due to feeding on plants infected with a semipersistently transmitted virus. Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) by the (Bemisia tabaci) semipersistent manner infects several globally important ornamental crops, including tomato. To determine changes global regulation whiteflies...
When infecting a host plant, the fungus Fusarium oxysporum secretes several effector proteins into xylem tissue to promote virulence. However, in plant with an innate immune system involving analogous resistance proteins, may trigger resistance, rather than promoting Identity of genes f. sp. niveum (Fon) races that affect watermelon (Citrullus lanatus) are currently unknown. In this study, SIX6 (secreted protein 6) gene was identified Fon 0 and 1 but not more virulent race 2. Disrupting...
The entire genome of grapevine leafroll-associated closterovirus-2 (GLRaV-2), except the exact 5' terminus, was cloned and sequenced. sequence encompasses nine open reading frames (ORFs) which include, in to 3' direction, an incomplete ORF1a encoding a putative viral polyprotein eight ORFs that encode proteins 52 kDa (ORF1b), 6 (ORF2), 65 (ORF3), 63 (ORF4), 25 (ORF5), 22 (ORF6), 19 (ORF7) 24 (ORF8) respectively, 216 nucleotides untranslated region. An potentially encoded large...
A single regeneration procedure using cotyledon explants effectively regenerated five commercially grown muskmelon cultivars. This scheme was used to facilitate gene transfers either Agrobacterium tumefaciens (using `Burpee Hybrid' and `Hales Best Jumbo') or microprojectile bombardment `Topmark') methods. In both cases, the transferred genes were from T-DNA region of binary vector plasmid pGA482GG/cp cucumber mosaic virus-white leaf strain (CMV-WL), which contains that encode neomycin...
Abstract The sweetpotato whitefly, Bemisia tabaci (Gennadius), is a worldwide pest of numerous agricultural and ornamental crops. In addition to directly feeding on plants, it also acts as vector plant viruses cultivated uncultivated host species. Moreover, plants can affect the population dynamics whiteflies. An open‐choice screening experiment was conducted with B‐biotype B. diverse collection crops, weeds, other indigenous Five species were further evaluated in choice or no‐choice tests...
In recent years, a number of serious disease outbreaks caused by viruses and viroids on greenhouse tomatoes in North America have resulted significant economic losses to growers. The objectives this study were evaluate the effectiveness commercial disinfectants against mechanical transmission these pathogens, select with broad spectrum reactivity control general virus viroid diseases tomato production. A total 16 evaluated Pepino mosaic (PepMV), Potato spindle tuber (PSTVd), Tomato (ToMV),...
A loop-mediated isothermal amplification (LAMP) assay was developed for simple, rapid and efficient detection of Cucurbit leaf crumple virus (CuLCrV), one the most important begomoviruses that infects cucurbits worldwide. set six specific primers targeting a total 240 nt sequence regions in DNA CuLCrV were designed synthesized from infected tissues using real-time LAMP with Genie® III system, which further confirmed by gel electrophoresis SYBR™ Green I staining visual observation. The...
This study reports on the complete genome sequence of Grapevine leafroll-associated virus 3, type member genus Ampelovirus. The is 17 919 nt in size and contains 13 open reading frames (ORFs). Previously, 154 3'-terminal was reported. newly sequenced portion a 158 5' UTR, single papain-like protease methyltransferase-like (MT) domain. ORF1a encodes large polypeptide with molecular mass 245 kDa. With predicted +1 frameshift, fusion protein generated from ORF1a/1b would produce 306 kDa...