A5085635512- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Advances in Cucurbitaceae Research
- Plant Molecular Biology Research
- Wheat and Barley Genetics and Pathology
- Plant Virus Research Studies
- Cocoa and Sweet Potato Agronomy
- Plant-Microbe Interactions and Immunity
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogenic Bacteria Studies
- Postharvest Quality and Shelf Life Management
- Bioenergy crop production and management
- Genomics and Phylogenetic Studies
- Plant pathogens and resistance mechanisms
- Protease and Inhibitor Mechanisms
- Plant and Fungal Interactions Research
- Genetics and Plant Breeding
- Photosynthetic Processes and Mechanisms
- Powdery Mildew Fungal Diseases
- Mycotoxins in Agriculture and Food
- Plant responses to water stress
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
United States Department of Agriculture
2018-2021
Agricultural Research Service
2018-2021
Washington State University
2012-2019
Michigan State University
2005-2015
Institute of Molecular and Cell Biology
2002
The Cucurbitaceae family (cucurbit) includes several economically important crops, such as melon, cucumber, watermelon, pumpkin, squash and gourds. During the past years, genomic genetic data have been rapidly accumulated for cucurbits. To store, mine, analyze, integrate disseminate these large-scale datasets to provide a central portal cucurbit research breeding community, we developed Cucurbit Genomics Database (CuGenDB; http://cucurbitgenomics.org) using Tripal toolkit. database currently...
Abstract Background Early stages of fruit development from initial set through exponential growth are critical determinants size and yield, however, there has been little detailed analysis this phase development. In study we combined morphological with 454 pyrosequencing to transcript level changes occurring in young cucumber at five ages anthesis the end growth. Results The samples produced 1.13 million ESTs which were assembled into 27,859 contigs a mean length 834 base pairs 67 reads per...
Type XV and type XVIII collagens are classified as part of multiplexin collagen superfamily their C-terminal parts, endostatin restin, respectively, have been shown to be anti-angiogenic in vivo vitro. The α1(XV) α1(XVIII) chains reported localized mainly the basement membrane zone, but distributions blood vessels nonvascular tissues yet thoroughly clarified. In present study, we raised monoclonal antibodies against synthetic peptides human used them for extensive investigation distribution...
Fruit development proceeds from cell division to expansion, maturation, and ripening. Expansion is critical for size, yield, quality; however, this period of has received little attention. We used 454-pyrosequencing develop a cucumber ( Cucumis sativus ) fruit transcriptome, identify highly expressed transcripts, characterize key functions during exponential growth. The resulting 187,406 sequence tags (ESTs) were assembled into 13,878 contigs. Quantitative real-time polymerase chain reaction...
Identification and utilization of resistance to Phytophthora capsici could provide the basis for a viable management strategy against cucumber fruit rot, persistent threat in (Cucumis sativus) production. Our objectives were develop method testing detached, nonwounded, P. capsici, screen cultivars plant introductions (collectively referred as cultigens) resistance. Four isolates (differing their sensitivity fungicide mefenoxam compatibility type) compared infection capability 1999 2000. No...
Abstract Yield and quality losses due to disease are major factors limiting crop productivity; thus breeding for resistance is a primary goal many plant programmes. Unfortunately, specific sources of not always available. In cases where genetic limited or non-existent, manipulating architecture facilitate avoidance may be valuable alternate approach mitigate severity. Modifications can used reduce contact with the pathogen, create barriers pathogen growth development, an unfavourable...
Phytophthora capsici causes severe losses in vegetable production, including many cucurbit crops. Our previous work showed that cucumber ( Cucumis sativus ) fruit are most susceptible to P. when they very young and rapidly elongating, but develop resistance as approach full length at 10 12 days postpollination (DPP). In this study, from seven additional crops representing four species, melon melo ), butternut squash Cucurbita moschata watermelon Citrullus lanatus zucchini, yellow summer...
Downy mildew, a foliar disease caused by the oomycete Pseudoperonospora cubensis (Berk. and Curt.) Rostow, is one of most destructive pathogens cucurbits. From 1961 to 2003, resistant cucumber cultivars in United States had sufficient resistance grow successful crop without use fungicides. The pathogen resurged as major problem 2004. Since then, dm-1 gene has not been effective against new strain downy yield losses are high objective this experiment was identify having mildew. 86 breeding...
Stripe rust ( Puccinia striiformis Westend f. sp. tritici ) is a devastating disease for wheat Triticum aestivum L.) production worldwide. Yr5 race‐specific resistance gene, effective to all races that have been identified in the United States. Therefore, it has increasingly used US cultivar development. The goal of this study was identify and validate single‐nucleotide polymorphism (SNP) markers tightly linked with , evaluate their usefulness diverse set materials, dissect region search...
Melon (C. melo L.) is an economically important vegetable crop cultivated worldwide. The melon collection in the U.S. National Plant Germplasm System (NPGS) a valuable resource to conserve natural genetic diversity and provide novel traits for breeding. Here we use genotyping-by-sequencing (GBS) technology characterize 2083 accessions NPGS collected from major production areas as well regions where primitive melons exist. Population structure analyses suggested that C. ssp. was firstly...
Very young cucumber (Cucumis sativus) fruit are highly susceptible to infection by the oomycete pathogen, Phytophthora capsici. As complete exponential growth, at approximately 10–12 days post pollination (dpp), they transition resistance. The development of age-related resistance (ARR) is increasingly recognized as an important defense against pathogens, however, underlying mechanisms largely unknown. Peel sections from harvested 8 dpp (susceptible) and 16 (resistant) showed equivalent...
Northern areas of the western United States are one most productive wheat growing regions in States. Increasing productivity through breeding is hindered by several biotic stresses which slow and constrain targeted yield improvement. In order to understand genetic variation for stripe rust (Puccinia striiformis f. sp. tritici), Septoria tritici blotch (Mycosphaerella graminicola), Hessian fly (Mayetiola destructor) regional germplasm, a panel 408 elite spring lines was characterized...
Fruit rot induced by Phytophthora capsici Leonian is an increasingly serious disease affecting pickling cucumber ( Cucumis sativus L.) production in many parts of the United States. The absence genetically resistant cultivars and rapid development fungicide resistance makes it imperative to develop integrated management strategies. Cucumber fruit which come direct contact with soil-borne pathogen are usually located under canopy where moist warm conditions favor development. We sought...
Before beginning a discussion about the future of plant breeding training, it is necessary to defi ne what breeder is. The traditional view breeders includes only those researchers who develop new plants that result in product more suited human needs. However, many feel this nition should be expanded include scientists contribute crop improvement through any type genetic studies. These studies range from using molecular markers describe levels diversity population identifying QTL underlie...
Cucurbit yellow stunting disorder virus (CYSDV) is a devastating viral disease of melon that can cause significant yield and quality losses. This has recently emerged as major concern in the southwest United States melon-growing regions across world. Coinfection by chlorotic yellows (CCYV) was recognized Imperial Valley neighboring production areas California Arizona 2018, but its importance remains largely unknown. Identifying deploying CYSDV resistance from elite germplasm an economical...
Dasypyrum villosum is a wild relative of common wheat (Triticum aestivum L.) with resistance to Puccinia graminis f. tritici, the causal agent stem rust, including highly virulent race TTKSK (Ug99). In order transfer resistance, T. durum-D. amphiploids were initially developed and used as bridge create wheat-D. introgression lines. Conserved ortholog set (COS) markers identify D. chromosome lines, which then subjected seedling P. tritici screening TTKSK. A COS marker-verified line carrying...
Cucumber PI308916 has a compact growth habit (short internodes and main stem length) due to single recessive gene cp . We also observed that this PI lower incidence of Phytophthora fruit rot, likely its upright bearing habit. Previous researchers reported cucumber lines derived from exhibited the potential for higher yields increased planting density. Despite these potentially beneficial traits, efforts develop PI308916-derived were terminated poor seedling establishment. The objective...
Phytophthoracapsici , a soil-borne oomycete pathogen causing fruit rot in cucumber, has become limiting factor for cucumber production the Midwest. In process of screening plant introductions (PIs) resistance to P. capsici it appeared that degree susceptibility might decrease as fruits develop. To examine this more carefully, detached, greenhouse-grown, hand-pollinated `Vlaspik' aged 2–18 days post-pollination (dpp) were inoculated with mycelium and evaluated symptoms. There was reproducible...