A5008475466- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Chromatography
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Chemistry and Sensors
- Mass Spectrometry Techniques and Applications
- Analytical Methods in Pharmaceuticals
- Microfluidic and Bio-sensing Technologies
- Advanced Chemical Sensor Technologies
- Chromatography in Natural Products
- Cancer therapeutics and mechanisms
- Electrochemical sensors and biosensors
- Electrostatics and Colloid Interactions
- Antibiotics Pharmacokinetics and Efficacy
- Protein purification and stability
- Nanopore and Nanochannel Transport Studies
- Biochemical Analysis and Sensing Techniques
- Spectroscopy and Quantum Chemical Studies
- Poisoning and overdose treatments
- Effects and risks of endocrine disrupting chemicals
- Analytical chemistry methods development
- Forensic Toxicology and Drug Analysis
- Carbohydrate Chemistry and Synthesis
- Pharmaceutical and Antibiotic Environmental Impacts
- Electrochemical Analysis and Applications
- Advanced Proteomics Techniques and Applications
Charles University
2011-2022
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2010
Czech Academy of Sciences, Institute of Analytical Chemistry
2001
Two constructions of the high-frequency contactless conductivity detector that are fitted to specific demands capillary zone electrophoresis described. The axial arrangement electrodes cell with two cylindrical placed around outer wall column is used. We propose an equivalent electrical model cell, which explains features its behavior including overshooting phenomena. give computer numerical solution enabling simulation real experimental runs. role many parameters can be evaluated in this...
Twenty underivatized essential amino acids were separated using capillary zone electrophoresis and consequently detected with contactless conductivity detection (CCD). A simple acidic background electrolyte (BGE) containing 2.3 M acetic acid 0.1% w/w hydroxyethylcellulose (HEC) allowed the electrophoretic separation sensitive of all 20 in their cationic form. The addition HEC to BGE suppressed both, electroosmotic flow analyte adsorption on surface resulting an excellent migration time...
Charge reversal as an extreme case of charge compensation is directly observed by capillary electrophoresis for a negatively charged peptide in aqueous solutions trivalent cations. Atomistic and coarse-grained simulations provide molecular interpretation this effect showing that it largely electrostatic origin with minor contribution chemical specificity the salt ions.
Using capillary electrophoresis and molecular dynamics simulations, we directly demonstrate that guanidinium cations exhibit an affinity for the positively charged side chains of arginine-containing peptides in aqueous solutions, while no such effect is observed sodium and/or lysine. Such a counterintuitive pairing between these two moieties has implications destabilization aggregation arginine-rich proteins salt solutions.
In the present study, we characterize binding of divalent cations to insulin in aqueous salt solutions by means capillary electrophoresis and molecular dynamics simulations. The results show a strong pH dependence. At low pH, at which all carboxylate groups are protonated protein has an overall positive charge, exhibit only weak rather unspecific interactions with insulin. contrast, close neutral when deprotonated negatively charged, charge-neutralizing effect magnesium, calcium, zinc,...
Domains rich in cationic amino acids are ubiquitous peptides with the ability to cross cell membranes, which is likely related binding of such polypeptides anionic groups on membrane surface. To shed more light these interactions, we investigated specific interactions between basic and oligopeptides thereof anions by means electrophoretic experiments molecular dynamics simulations. this end, measured mobilities arginine, lysine, tetraarginine, tetralysine sodium chloride sulfate electrolytes...
Vernix caseosa protects the skin of a human fetus during last trimester pregnancy and newborn after delivery. Besides its cellular proteinaceous components, an important constituent functional agent is complex lipid fraction, implicated in multitude salubrious effects vernix caseosa. Little known about how chemical composition lipids affected by various biological characteristics baby, such as gestational age, birth weight, and, but not least, gender newborn. This study reports on...
Abstract Four methacrylate ester‐based monolithic columns for capillary liquid chromatography (CLC) were prepared by radical polymerization with ammonium peroxodisulfate (3 columns) and thermal initiation (1 column). The mixture consisted of butyl (BMA) ethylene glycol dimethacrylate (EDMA), propan‐1‐ol, butane‐1,4‐diol, water, as initiator. It was necessary to add N,N,N ′, N ′‐tetramethylethylenediamine (TEMED) the activate reaction. amount initiator activator optimized attain quantitative...
The cationic sensitizer 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porphyrin (TMPyP) forms supramolecular complexes with native, per-methylated, sulfonated and dimethyl-sulfonated cyclodextrins (CDs). Binding interactions were proved by NMR, mass spectra, capillary zone electrophoresis, UV-Vis fluorescence spectroscopy. 2D-NMR experiments on native CDs indicate that the interaction of TMPyP external CD surface is dominant binding mode. high affinity towards due to electrostatic...