A5036728629- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Respiratory viral infections research
- Virology and Viral Diseases
- Microbial Community Ecology and Physiology
- Bacillus and Francisella bacterial research
- Vibrio bacteria research studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- COVID-19 epidemiological studies
- Microfluidic and Capillary Electrophoresis Applications
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Biosensors and Analytical Detection
Swiss Federal Institute of Aquatic Science and Technology
2023-2025
ETH Zurich
2023
Massachusetts Institute of Technology
2023
École Polytechnique Fédérale de Lausanne
2019
Waterborne human viruses can persist in the environment, causing a risk to health over long periods of time. In this work, we demonstrate that both freshwater and seawater environments, indigenous bacteria protists graze on waterborne thereby reduce their persistence. We furthermore efficiency grazing process depends temperature, virus type, protist species. These findings may facilitate design biological methods for disinfection water wastewater.
Monkeypox virus (MPXV) is a zoonotic pathogen that has recently caused outbreaks in non-endemic areas. Wastewater-based surveillance (WBS) offers substantial potential for monitoring MPVX clades, and it can inform population-level disease dynamics. Here, we report four-plex digital PCR assay to detect quantify different clades subclades of MPXV. The demonstrated specificity distinguishing quantifying MPXV by clade, which advantageous application both clinical wastewater settings.
Measles outbreaks remain a significant public health challenge despite high vaccination coverage in many regions. Wastewater-based surveillance (WBS) offers non-invasive and community-level approach to monitoring the circulation of pathogens, including measles virus. Here, we retrospectively applied duplex digital PCR assay distinguish between wild-type vaccine strains virus wastewater samples available from an existing national WBS program. Samples originated treatment plant serving...
As more data on virus concentrations in influent water from wastewater treatment plants (WWTPs) becomes available, establishing best practices for measurements, monitoring, and statistical modelling can improve the understanding of concentration distributions wastewater. To support this, we assessed temporal variability norovirus, adenovirus, enterovirus, rotavirus across multiple WWTPs Switzerland, USA, Japan. Our findings demonstrate that lognormal distribution accurately predicts...
Monkeypox virus (MPXV) is a zoonotic pathogen that has recently caused outbreaks in non-endemic areas. Wastewater-based surveillance (WBS) offers substantial potential for monitoring MPVX clades, and it can inform population-level disease dynamics. Here, we report four-plex digital PCR assay to detect quantify different clades subclades of MPXV. The demonstrated specificity distinguishing quantifying MPXV by clade, which advantageous application both clinical wastewater settings.
Noroviruses and enteroviruses are major causes of endemic gastrointestinal disease associated with substantial burden. However, viral gastroenteritis is often diagnosed based on symptoms, etiology infrequently tested or reported, so little information exists community-level transmission dynamics. In this study, we demonstrate that norovirus (NoV) genogroup II enterovirus (EV) loads in wastewater reveal dynamics these viruses. We report NoV EV concentrations from 363 samples between December...
ABSTRACT Wastewater-based surveillance systems can track trends in multiple pathogens simultaneously by leveraging efficient, streamlined laboratory processing. In Switzerland, wastewater is conducted for fourteen locations representing 2.3 million people, or 26% of the national population, with simultaneous four respiratory pathogens. Trends diseases are tracked using a novel, six-plex digital PCR assay targeting Influenza A, B, Respiratory Syncytial Virus, and SARS-CoV-2 N1 N2 genes, as...
Abstract Quantitative PCR (qPCR) and digital (dPCR) are applied for quantifying molecular targets in disease diagnostics, pathogen detection ecological monitoring. Uptake of dPCR is increasing due to its higher quantification accuracy relative qPCR which stems from independence standard curves increased resistance inhibitors. Throughput can be through multiplexing, allows simultaneous multiple targets. However, multiplexing with faces unique challenges qPCR. Here we describe the three-phase...
Abstract The COVID-19 pandemic has accelerated the development and adoption of wastewater-based epidemiology. Wastewater samples can provide genomic information for detecting assessing spread SARS-CoV-2 variants in communities estimating important epidemiological parameters such as growth advantage variant. However, despite demonstrated successes, data derived from wastewater suffers potential biases. Of particular concern are differential shedding profiles that different exhibit, because...
Abstract The COVID-19 pandemic has accelerated the development and adoption of wastewater-based epidemiology. Wastewater samples can provide genomic information for detecting assessing spread SARS-CoV-2 variants in communities estimating important epidemiological parameters such as growth advantage variant. However, despite demonstrated successes, data derived from wastewater suffers potential biases. Of particular concern are differential shedding profiles that different exhibit, because...