A5049943290- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Plant pathogens and resistance mechanisms
- Banana Cultivation and Research
- Genomics and Phylogenetic Studies
- Entomopathogenic Microorganisms in Pest Control
- Plant Parasitism and Resistance
- Insect Resistance and Genetics
- Plant tissue culture and regeneration
- Insect and Pesticide Research
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Plant Disease Resistance and Genetics
- Microbial Metabolism and Applications
- Metalloenzymes and iron-sulfur proteins
- Plant Disease Management Techniques
- Plant Micronutrient Interactions and Effects
- Fungal Plant Pathogen Control
- Microbial Metabolic Engineering and Bioproduction
- Statistics Education and Methodologies
- Food, Nutrition, and Cultural Practices
- Historical Studies in Science
- Renal cell carcinoma treatment
Universidad Publica de Navarra
2014-2025
Agrobiotechnology Institute
2000-2018
Institut de Seguretat Pública de Catalunya
2012
Instituto de Lengua Literatura y Antropología
2011
Cultura
2011
Empresa Nacional de Residuos Radiactivos
2011
Universidad de Extremadura
2011
Universidad de La Rioja
2011
NEIKER, the Basque Institute for Agricultural Research and Development
2008-2009
Tecnalia
2009
The 154-kb plasmid was cured from race 7 strain 1449B of the phytopathogen Pseudomonas syringae pv. phaseolicola ( Pph ). Cured strains lost virulence toward bean, causing hypersensitive reaction in previously susceptible cultivars. Restoration achieved by complementation with cosmid clones spanning a 30-kb region that contained identified avirulence avr ) genes avrD , avrPphC and avrPphF . Single transposon insertions at multiple sites (including one located abolished restoration genomic...
Bacterial pathogens exhibit significant variation in their genomic content of virulence factors. This reflects the abundance strategies evolved to infect host organisms by suppressing immunity. Molecular arms-races have been a strong driving force for evolution pathogenicity, with often encoding overlapping or redundant functions, such as type III protein secretion effectors and hosts ever more sophisticated immune systems. The pathogens' frequent exposure other microbes, either environment,...
Pseudomonas savastanoi pv. is a tumour-inducing pathogen of Olea europaea L. causing olive knot disease. Bioinformatic analysis the draft genome sequence strain NCPPB 3335, which encodes 5232 predicted coding genes on total length 5856 998 bp and 57.12% G + C, revealed large degree conservation with syringae phaseolicola 1448A P. tabaci 11528. However, 3335 contains twelve variable genomic regions, are absent in all previously sequenced strains. Various features that could contribute to...
ABSTRACT Bacterial apical necrosis of mango, elicited by Pseudomonas syringae pv. syringae, limits fruit production in southern Spain and Portugal. Examination a collection P. isolates for copper resistance showed that 59% were resistant to cupric sulfate. The survey mango orchard revealed an increase frequencies copper-resistant bacteria after repeated treatments with Bordeaux mixture. These data suggest selection strains could be major reason control failures following management...
The effect of the plasmid‐encoded rulAB (resistance to ultraviolet radiation) determinant on responses Pseudomonas syringae ultraviolet‐B (UV‐B) radiation and distribution among pathovars P. were determined. cloned native + plasmid pPSR1 both conferred approximately a 10‐fold increase in survival pv. FF5 following increasing doses UV‐B radiation. strains also maintained significantly larger epiphytic populations leaf surfaces irradiated with UV‐B. ‐insertional mutants, constructed two...
The nucleotide sequence of a 2.5-kbp region following the hydrogenase structural genes (hupSL) in H2 uptake gene cluster from Rhizobium leguminosarum bv. viciae UPM791 was determined. Four closely linked encoding peptides 27.9 (hupC), 22.1 (hupD), 19.0 (hupE), and 10.4 (hupF) kDa were identified immediately downstream hupL. Proteins with comparable apparent molecular weights detected by heterologous expression these Escherichia coli. six genes, hupS to hupF, are arranged as an operon, mutant...
This study describes the insecticidal activity of a novel Bacillus thuringiensis Cry-related protein with deduced 799 amino acid sequence (~89 kDa) and ~19% pairwise identity to 95-kDa-aphidicidal (sequence number 204) from patent US 8318900 ~40% cancer cell killing Cry proteins (parasporins Cry41Ab1 Cry41Aa1), respectively. contained five conserved blocks three domains commonly found in 3-domain proteins. The exhibited toxic against green peach aphid, Myzus persicae (Sulzer) (Homoptera:...
Pseudomonas savastanoi pv. NCPPB 3335 causes olive knot disease and is a model pathogen for exploring bacterial infection of woody hosts. The type III secretion system (T3SS) effector repertoire this strain includes 31 candidates plus two novel identified in study which have not been reported to translocate into plant cells. In work, we demonstrate the delivery seven effectors Nicotiana tabacum leaves, including three proteins from families P. syringae complex super-repertoire (HopBK HopBL),...
Mangotoxin is an antimetabolite toxin produced by certain Pseudomonas syringae pv. strains. This oligopeptide that inhibits ornithine N-acetyl transferase, a key enzyme in the biosynthesis of and arginine. Previous studies have reported involvement putative nonribosomal peptide synthetase MgoA virulence mangotoxin production. In this study, we analyse new chromosomal region P. UMAF0158, which contains six coding sequences arranged as operon (mbo operon). The mbo was detected only...
From a collection of 152 pseudomonads isolated from diseased beans in Spain, 138 (91%) the strains were identified as Pseudomonas syringae pv. phaseolicola and rest P. syringae. The produced typical water-soaked lesions on bean pods, although 95 them did not produce phaseolotoxin vitro. Ninety-four these isolates expected 0.5-kb product after polymerase chain reaction (PCR) amplification using primers specific for open reading frame (ORF) 6 (tox) gene cluster contain DNA homologous to ORF...
In symbiosis with peas, Rhizobium leguminosarum UPM791 induces the synthesis of a hydrogen uptake (Hup) system that recycles generated in nodules by nitrogenase. A cosmid (pAL618) containing hup genes from this strain on 20-kilobase-pair (kb) DNA insert has previously been isolated our laboratory (A. Leyva, J. M. Palacios, T. Mozo, and T.Ruiz-Argüeso, Bacteriol. 169:4929-4934, 1987). Here we show pAL618 contains all genetic information required to confer high levels hydrogenase activity...
ABSTRACT Pseudomonas savastanoi pv. strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of related species syringae examined and contribute ecological pathogenic fitness their host. However, there is a general lack information about gene content P. role interaction this pathogen with olive plants. We designed DNA macroarray containing 135 plasmid-borne genes conduct global genetic analysis 32 obtained from 10 strains. Hybridization...
Pseudomonas syringae pv. phaseolicola is the causal agent of halo blight disease beans (Phaseolus vulgaris L.), which characterized by water-soaked lesions surrounded a chlorotic resulting from action non-host-specific toxin known as phaseolotoxin. This phytotoxin inhibits enzyme ornithine carbamoyltransferase involved in arginine biosynthesis. Different evidence suggested that genes phaseolotoxin production were clustered. Two had been previously identified our laboratory within this...
Pseudomonas savastanoi pv. NCPPB 3335 is a model for the study of molecular basis disease production and tumor formation in woody hosts, its draft genome sequence has been recently obtained. Here we closed plasmid complement this strain, composed three circular molecules 78,357 nt (pPsv48A), 45,220 (pPsv48B), 42,103 (pPsv48C), all belonging to pPT23A-like family plasmids widely distributed P. syringae complex. A total 152 coding sequences were predicted complement, which 38 are hypothetical...
Mangotoxin is an antimetabolite toxin that produced by strains of Pseudomonas syringae pv. syringae; mangotoxin-producing are primarily isolated from mango tissues with symptoms bacterial apical necrosis. The oligopeptide inhibits ornithine N-acetyl transferase (OAT), a key enzyme in the biosynthetic pathway essential amino acids and arginine. involvement putative nonribosomal peptide synthetase gene (mgoA) mangotoxin production virulence has been reported. In present study, we performed...
The study of host range determinants within the Pseudomonas syringae complex is gaining renewed attention due to its widespread distribution in non-agricultural environments, evidence large variability intra-pathovar range, and emergence new epidemic diseases. This requires establishment appropriate model pathosystems facilitating integration phenotypic, genomic evolutionary data. savastanoi pv. a pathogen olive tree, here we report closed genome strain NCPPB 3335, plus draft sequences three...
GacS/GacA is a widely distributed two-component system playing an essential role as key global regulator, although its characterization in phytopathogenic bacteria has been deeply biased, being intensively studied pathogens of herbaceous plants but barely investigated woody hosts. P. savastanoi pv. (Psv) characterized by inducing tumours the stem and branches olive trees. In this work, model strain Psv NCPPB 3335 mutant derivative with complete deletion gene gacA were subjected to RNA-Seq...