A5045710304- Legume Nitrogen Fixing Symbiosis
- Gene expression and cancer classification
- Computational Drug Discovery Methods
- Peroxisome Proliferator-Activated Receptors
- Plant nutrient uptake and metabolism
- Chronic Kidney Disease and Diabetes
- Molecular Biology Techniques and Applications
- Adipose Tissue and Metabolism
- Biosimilars and Bioanalytical Methods
- Animal testing and alternatives
- Acute Kidney Injury Research
- RNA and protein synthesis mechanisms
- Veterinary Oncology Research
- Trace Elements in Health
- Pharmacogenetics and Drug Metabolism
- RNA Research and Splicing
- Virus-based gene therapy research
- Soybean genetics and cultivation
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Protein Degradation and Inhibitors
- Statistical Methods in Clinical Trials
- Metabolism, Diabetes, and Cancer
- Lipid Membrane Structure and Behavior
- Fungal Plant Pathogen Control
National Center for Advancing Translational Sciences
2016-2024
National Institutes of Health
2013-2024
United States Military Academy
1996-2021
Merck & Co., Inc., Rahway, NJ, USA (United States)
1999-2007
Thomas Jefferson University
1994-1997
University of California, Riverside
1989-1994
Washington State University
1993
University of Minnesota
1991
Institute of Genetics
1989
Hungarian Academy of Sciences
1989
Changes in gene expression can help reveal the mechanisms of disease processes and mode action for toxicities adverse effects on cellular responses induced by exposures to chemicals, drugs environment agents. The U.S. Tox21 Federal collaboration, which currently quantifies biological nearly 10,000 chemicals via quantitative high-throughput screening(qHTS) vitro model systems, is now making an effort incorporate profiling into existing battery assays. Whole transcriptome analyses performed...
PPARγ is an adipocyte-specific nuclear hormone receptor. Agonists of PPARγ, such as thiazolidinediones (TZDs), promote adipocyte differentiation and have insulin-sensitizing effects in animals diabetic patients. Affymetrix oligonucleotide arrays representing 6347 genes were employed to profile the gene expression responses mature 3T3-L1 adipocytes differentiating preadipocytes a TZD agonist vitro. The 579 was significantly up- or down-regulated by more than 1.5-fold during and/or treatment...
Oligonucleotide DNA microarrays were investigated for utility in measuring global expression profiles of drug metabolism genes. This study was performed to investigate the feasibility using microarray technology minimize long, expensive process testing candidates safety animals. In an evaluation hybridization specificity, from Affymetrix distinguished genes up a threshold ∼90% identity. Oligonucleotides representing human cytochrome P-450 gene CYP3A5 showed heterologous CYP3A4 and CYP3A7...
Several soybean genotypes have been identified which specifically exclude nodulation by members of Bradyrhizobium japonicum serocluster 123. We and sequenced a DNA region from B. strain USDA 110 is involved in genotype-specific soybeans. This 2.3-kilobase region, cloned pMJS12, allows 123 isolates to form nodules on plants serogroup 123-restricting genotypes. The nodules, however, were ineffective for symbiotic nitrogen fixation. nodulation-complementing located approximately 590 base pairs...
By using cloned Rhizobium meliloti, leguminosarum, and sp. strain MPIK3030 nodulation (nod) genes as hybridization probes, homologous regions were detected in the slow-growing soybean symbiont Bradyrhizobium japonicum USDA 110. These found to cluster within a 25-kilobase (kb) region. Specific nod probes from R. meliloti used identify nodA-, nodB-, nodC-, nodD-like sequences clustered on two adjacent HindIII restriction fragments of 3.9 5.6 kb. A 785-base-pair sequence was identified between...
An experimental and computational approach to categorizing UVCBs according chemical biological similarities.
Background: A central challenge in toxicity testing is the large number of chemicals commerce that lack toxicological assessment. In response, Tox21 program re-focusing from animal studies to less expensive and higher throughput vitro methods using target/pathway-specific, mechanism-driven assays. Objectives: Our objective was use an in-depth mechanistic study approach prioritize characterize affecting mitochondrial function. Methods: We used a tiered for more extensive 622 compounds...
Abstract More than 75 000 man‐made chemicals contaminate the environment; many of these have not been tested for toxicities. These demand quantitative high‐throughput screening assays to assess them causative roles in neurotoxicities, including Parkinson's disease and other neurodegenerative disorders. To facilitate high throughput cytotoxicity neurons, three human neuronal cellular models were compared: SH‐SY5Y neuroblastoma cells, LUHMES conditionally‐immortalized dopaminergic Neural Stem...
Abstract Canine osteosarcoma is increasingly recognized as an informative model for human osteosarcoma. Here we show in one of the largest clinically annotated canine transcriptional datasets that two previously reported, well de novo gene signatures devised through single sample Gene Set Enrichment Analysis (ssGSEA), have prognostic utility both and patients. Shared molecular pathway alterations are seen immune cell signaling activation including TH1 TH2 signaling, interferon inflammatory...
The androgen receptor (AR), when complexed with 5α-dihydrotestosterone (DHT), supports the survival and proliferation of prostate cells, a process critical for normal development, benign prostatic hypertrophy, tumorigenesis. However, androgen-responsive genetic pathways that control cell division differentiation are largely unknown. To identify such pathways, we examined gene expression in ventral 6 24 h after DHT administration to androgen-depleted rats. 234 transcripts were expressed...
The androgen receptor (AR) is expressed in the uterus; however, role of AR female reproductive physiology poorly understood. Here we examined effects androgens on uterine growth and gene expression adult ovariectomized rats. Nonaromatizable AR-selective agonists potently stimulate hypertrophy induce significant myometrial expansion distinct from that induced by 17beta-estradiol (E2). In endometrium, only modestly increase epithelial cell height antagonize trophic E2. To identify underlying...
The cell surface polysaccharides of wild-type Bradyrhizobium japonicum USDA 110 and a nonnodulating mutant, strain HS123, were analyzed. capsular polysaccharide (CPS) exopolysaccharide (EPS) the wild type mutant do not differ in their sugar composition. CPS EPS are composed mannose, 4-O-methylgalactose/galactose, glucose, galacturonic acid ratio 1:1:2:1, respectively. H nuclear magnetic resonance spectra very similar, but identical, suggesting minor structural variation these...
Drug-induced phospholipidosis is characterized by the accumulation of intracellular phospholipids in cells exposed to cationic amphiphilic drugs. The appearance unicentric or multicentric multilamellar bodies viewed under an electron microscope (EM) morphological hallmark phospholipidosis. Although EM method gold standard for detecting cellular phospholipidosis, this has its drawbacks, including low throughput, high cost, and unsuitability screening a large chemical library. In study,...