A5004443381- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Lung Cancer Treatments and Mutations
- CNS Lymphoma Diagnosis and Treatment
- Neuroscience of respiration and sleep
- RNA modifications and cancer
- Veterinary Equine Medical Research
- Lymphoma Diagnosis and Treatment
- Glaucoma and retinal disorders
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neuropeptides and Animal Physiology
- Immune cells in cancer
- Gastric Cancer Management and Outcomes
- CAR-T cell therapy research
- Systemic Lupus Erythematosus Research
- Ocular Diseases and Behçet’s Syndrome
- Gastrointestinal motility and disorders
- Immunotherapy and Immune Responses
- Retinal Development and Disorders
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Retinal Imaging and Analysis
- Nitric Oxide and Endothelin Effects
- Retinal and Optic Conditions
- Pain Mechanisms and Treatments
Duke University
2025
Duke Medical Center
2023
Stanford University
2013-2023
Cornell University
2017-2022
University of California, San Diego
2014-2021
Bristol-Myers Squibb (United States)
2021
National Institutes of Health
2016-2020
National Center for Advancing Translational Sciences
2016-2020
National Eye Institute
2016-2019
Kettering University
2015-2019
Macrophages promote fibroblast invasiveness and demonstrate variable response to anti-inflammatory medications in patients with rheumatoid arthritis.
Deep sequencing based ribosome footprint profiling can provide novel insights into the regulatory mechanisms of protein translation. However, observed profile is fundamentally confounded by transcriptional activity. In order to decipher principles translation regulation, tools that reliably detect changes in efficiency case-control studies are needed.We present a statistical framework and an analysis tool, RiboDiff, genes with across experimental treatments. RiboDiff uses generalized linear...
Abstract The segmental and central distributions of renal nerve afferents in adult cats kittens were studied by using retrograde transganglionic transport horseradish peroxidase (HRP). Transport HRP from the cut ends left nerves labeled afferent axons ipsilateral minor splanchnic sensory perikarya dorsal root ganglia T12 to L4. majority cells (85%) located between L1 L3. A few neurons contralateral also labeled. Labeled not confined any particular region within a ganglion. Some examples...
Abstract Anatomical and electrophysiological experiments have demonstrated a prominent projection from the sacral sympathetic chain via pelvic nerve to postganglionic nerves on surface of urinary bladder Large intestine cat. Retrograde labeling studies revealed that nerve, which is generally believed carry primarily parasympathetic axons, has considerable population fibers originating mainly S1‐S3 paravertebral ganglia. The number neurons projecting (2,100) was about.75% pudendal (2,900),...
Abstract More than 75 000 man‐made chemicals contaminate the environment; many of these have not been tested for toxicities. These demand quantitative high‐throughput screening assays to assess them causative roles in neurotoxicities, including Parkinson's disease and other neurodegenerative disorders. To facilitate high throughput cytotoxicity neurons, three human neuronal cellular models were compared: SH‐SY5Y neuroblastoma cells, LUHMES conditionally‐immortalized dopaminergic Neural Stem...
Splanchnic afferent projections to the spinal cord and gracile nucleus were labeled following application of HRP central cut end major splanchnic nerve. Labeled fibers detected in ipsilateral dorsal column, Lissauer's tract (LT), laminae 1, 5, 7, 10, gray commissure at T1-T13 levels cord. Afferent not identified 2-4. Collaterals from LT projected ventrally along lateral medial margins horn (called pathways, respectively). Afferents pathway formed small bundles, spaced rostrocaudally...
Abstract Anterograde and retrograde tracing techniques were employed to delineate the organization of a visual cortical input deep layers hamster's superior colliculus which may be mediated by links in striatum substantia nigra. Autoradiographic experiments showed that areas 17, 18a, cortex medial area 17 (areas 18b 29) all projected dorsocaudal part ipsilateral striatum. This projection was organized so rostrocaudal axis represented along anteroposterior Large posterior neocortical...
Abstract The fiber compositio of the left major splanchnic nerve was studed in cats by electron microscopy. Comparisons were made between normal and partically degenerated specimens following ventral rhizotomy (T3‐L1), or spinal division (T3‐L1). Normal, nerves contained 2,500–4,000 myelinated 10,000–15,000 unmyelinated fibers. Preganglionic fibers included approximately 90% finely (1–7μm) over 50% Removal sensory preganglionic components revealed a third, postganglionic category. This 13–38...
The tau gene encodes a microtubule-associated protein that is critical for neuronal survival and function.Splicing defects in the human lead to frontotemporal dementia with Parkinsonism linked chromosome 17 (FTDP-17), an autosomal dominant neurodegenerative disorder.Genetic mutations associated FTDP-17 often affect exon 10 alternative splicing.To investigate mechanisms regulating splicing, we have developed green fluorescent reporter skipping expression cloning strategy identify splicing...
While the availability of public internet-scale datasets images and language has catalyzed remarkable progress in machine learning, medical are constrained by regulations protecting patient privacy time cost required for curation labeling. Self-supervised learning or pretraining demonstrated great success meaningful representations from large unlabeled to enable efficient on downstream tasks. In ophthalmology, RETFound model, a vision transformer (ViT-L) model trained masked autoencoding 1.6...
BACKGROUND: Poor muscle health is an adverse prognostic factor in clear cell renal cancer (ccRCC), but mechanisms are unknown. We examined associations among six body composition features, ClearCode34 tumor molecular subtype, and survival. METHODS: In a cohort of 837 patients with localized ccRCC, we segmented pre-surgical CT scans for cross-sectional areas radiodensities skeletal muscle, visceral adiposity, subcutaneous adiposity. RNA was extracted from archived specimens run on custom...
Abstract The intercollicular pathway of the hamster was studied by means a combination horseradish peroxidase (HRP), autoradiographic, and double‐labeling (nuclear yellow‐HRP) techniques. Small deposits HRP marked significant numbers cells in contralateral colliculus only when injection site included laminae ventral to stratum opticum. Anterior labeled many more neurons than injections into caudal part tectum. Of our injections, 18.2% were located superficial collicular (stratum griseum...
During rheumatoid arthritis (RA), Tumor Necrosis Factor (TNF) activates fibroblast-like synoviocytes (FLS) inducing in a temporal order constellation of genes, which perpetuate synovial inflammation. Although the molecular mechanisms regulating TNF-induced transcription are well characterized, little is known about impact mRNA stability on gene expression and TNF decay rates transcripts FLS. To address these issues we performed RNA sequencing genome-wide analysis stabilome RA We found that...
Aim: To investigate the cost–effectiveness of afatinib and erlotinib as second-line therapy for advanced squamous cell carcinoma lung. Materials & methods: A decision-analytic model was developed projecting economic outcomes. Clinical parameters utilities were from LUX-Lung 8 trial. Costs mainly estimated Chinese health system. The outcome incremental ratio. Results: strategy generated additional 0.154 quality-adjusted life-years compared with erlotinib, costs ¥16,852. Relative to resulted...