A5100751138- RNA Research and Splicing
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- RNA and protein synthesis mechanisms
- Retinoids in leukemia and cellular processes
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Viral-associated cancers and disorders
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Renal Transplantation Outcomes and Treatments
- Transplantation: Methods and Outcomes
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- T-cell and B-cell Immunology
- T-cell and Retrovirus Studies
- interferon and immune responses
- Ubiquitin and proteasome pathways
- CAR-T cell therapy research
- Cancer-related gene regulation
- Hemoglobinopathies and Related Disorders
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
National Heart Lung and Blood Institute
2016-2025
Anhui Agricultural University
2025
National Institutes of Health
2014-2024
Xinjiang Institute of Materia Medica
2024
Institute of Chinese Materia Medica
2024
Shanghai Traditional Chinese Medicine Hospital
2024
Shanghai CASB Biotechnology (China)
2024
Shanghai First People's Hospital
2019-2024
Shanghai Jiao Tong University
2014-2024
Peking University
2011-2023
Diffuse large B-cell lymphoma (DLBCL) is the most common form of in adults. The disease exhibits a striking heterogeneity gene expression profiles and clinical outcomes, but its genetic causes remain to be fully defined. Through whole genome exome sequencing, we characterized diversity DLBCL. In all, sequenced 73 DLBCL primary tumors (34 with matched normal DNA). Separately, exomes 21 cell lines. We identified 322 cancer genes that were recurrently mutated DLBCLs. recurrent mutations...
Promyelocytic leukemia (PML) is the organizer of nuclear matrix domains, PML bodies (NBs), with a proposed role in apoptosis control. In acute promyelocytic leukemia, PML/retinoic acid receptor (RAR) α expression disrupts NBs, but therapies such as retinoic or arsenic trioxide (As2O3) restore them. conjugated by ubiquitin-related peptide SUMO-1, process enhanced As2O3 and to target matrix. We demonstrate that triggers proteasome-dependent degradation PML/RARα this requires specific...
Acute promyelocytic leukemia (APL) is associated with the t(15;17) translocation, which generates a PML/RARα fusion protein between PML, growth suppressor localized on nuclear matrix-associated bodies, and RARα, receptor for retinoic acid (RA). was proposed to block myeloid differentiation through inhibition of response, as does dominant negative RARα mutant. In addition, in APL cells, displaces PML other body (NB) antigens onto microspeckles, likely resulting loss and/or NB functions. RA...
Myeloid-derived suppressor cells (MDSCs) are a heterogeneous family of myeloid that suppress T cell immunity in tumor-bearing hosts. In patients with colon cancer, MDSCs have recently been described as Lin−/lowHLA-DR−CD11b+CD33+ correlating cancer stage, metastasis and chemotherapy response. To learn more detail the dynamic change clinical relevance circulating tumor-infiltrating MDSC colorectal we harvested blood from 64 varying stage tumor matched paraneoplastic tissues 5 advanced...
“Please release me, let me go.” RNA polymerase II (Pol II) is the principal protein complex required for gene transcription in metazoan cells. Many genes have a “paused” Pol near their promoters, waiting to be released so they can start messenger synthesis. Yu et al. show that II–associated factor 1 (PAF1) plays central role regulating activation of these paused complexes. The positive elongation b helps recruit PAF1 II. This facilitates phosphorylation on its C-terminal domain, freeing it...
Epigenetic memory for signal-dependent transcription has remained elusive. So far, the concept of epigenetic been largely limited to cell-autonomous, preprogrammed processes such as development and metabolism. Here we show that IFNβ stimulation creates transcriptional in fibroblasts, conferring faster greater upon restimulation. The was inherited through multiple cell divisions led improved antiviral protection. Of ∼2,000 IFNβ-stimulated genes (ISGs), about half exhibited memory, which...
The application of deep sequencing to map 5′ capped transcripts has confirmed the existence at least two distinct promoter classes in metazoans: "focused" promoters with transcription start sites (TSSs) that occur a narrowly defined genomic span and "dispersed" TSSs are spread over larger window. Previous studies have explored presence features, such as CpG islands sequence motifs, these classes, but virtually no directly investigated relationship chromatin features. Here, we show...
Transcripts in platelets are largely produced precursor megakaryocytes but remain physiologically active as translate RNAs and regulate protein/RNA levels. Recent studies using transcriptome sequencing (RNA-seq) characterized the platelet limited number of non-diseased individuals. Here, we expand upon these RNA-seq by completing from 32 patients with acute myocardial infarction (MI). Our goals were to characterize a population MI relate gene expression aggregation measures ST-segment...
Allograft failure is common in lung-transplant recipients and leads to poor outcomes including early death. No reliable clinical tools exist identify patients at high risk for allograft failure. This study tested the use of donor-derived cell-free DNA (%ddcfDNA) as a sensitive marker graft injury predict impending failure.This multicenter, prospective cohort enrolled 106 subjects who underwent lung transplantation monitored them after development (defined severe chronic dysfunction [CLAD],...
Mixed-lineage leukemia ( MLL ) fusions are potent oncogenes that initiate aggressive forms of acute leukemia. As aberrant transcriptional regulators, MLL-fusion proteins alter gene expression in hematopoietic cells through interactions with the histone H3 lysine 79 (H3K79) methyltransferase DOT1L. Notably, interference cofactors like DOT1L is an emerging therapeutic strategy this disease. Here, we identify H2B E3 ubiquitin ligase ring finger protein 20 (RNF20) as additional chromatin...
Article6 March 2019Open Access Transparent process BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses Anup Dey Division of Developmental Biology, National Institute Child Health Human Development, Bethesda, MD, USAThis article has been contributed to by US Government employees their work is in the public domain USA Search for more papers this author Wenjing Yang The DNA Sequencing Computational Heart, Lung Blood Institute, Anne Gegonne...
T cell activation is a well-established model for studying cellular responses to exogenous stimulation. Using strand-specific RNA-seq, we observed that intron retention prevalent in polyadenylated transcripts resting CD4(+) cells and significantly reduced upon activation. Several lines of evidence suggest intron-retained are less stable than fully spliced transcripts. Strikingly, the decrease (IR) levels correlate with increase steady-state mRNA levels. Further, majority genes upregulated...
The SWI/SNF complex plays an important role in mouse embryonic stem cells (mESCs), but it remains to be determined whether this is required for the pluripotency of human ESCs (hESCs). Using RNAi, we demonstrated that depletion BRG1, catalytic subunit complex, led impaired self-renewing ability and dysregulated lineage specification hESCs. A unique composition BRG1-SWI/SNF hESCs was further defined by presence BAF250A, BAF170, BAF155, BAF53A, BAF47. Genome-wide expression analyses revealed...