A5101703145- Ubiquitin and proteasome pathways
- Immunotherapy and Immune Responses
- Endoplasmic Reticulum Stress and Disease
- Reproductive biology and impacts on aquatic species
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Potassium and Related Disorders
- Genomics and Chromatin Dynamics
- Pharmaceutical and Antibiotic Environmental Impacts
- Hepatitis Viruses Studies and Epidemiology
- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Wnt/β-catenin signaling in development and cancer
- Chromatin Remodeling and Cancer
- Connective tissue disorders research
- Inflammasome and immune disorders
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Hepatitis B Virus Studies
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Marine and fisheries research
- CRISPR and Genetic Engineering
- Cell Adhesion Molecules Research
- Enzyme Structure and Function
Shanghai Ninth People's Hospital
2023-2025
Shanghai Jiao Tong University
2019-2025
Cell Technology (China)
2025
Soochow University
2023
Universität Hamburg
2021
East China Normal University
2014
Background/Objectives: In chronic hepatitis B infection (CHB), the surface antigen (HBsAg) continuously exhausts antibody (HBsAb), which leads to formation of immune tolerance. Accordingly, virus (HBV) can be blocked by inhibiting binding pre-S1/pre-S2 hepatocyte receptor NTCP, but clinical cure rate pre-S-based vaccines for CHB is limited. Methods: this study, we designed and prepared multivalent therapeutic mRNA encoding three proteins (L, M, S) at cell membrane, verified via in vitro...
The SWI/SNF complex plays an important role in mouse embryonic stem cells (mESCs), but it remains to be determined whether this is required for the pluripotency of human ESCs (hESCs). Using RNAi, we demonstrated that depletion BRG1, catalytic subunit complex, led impaired self-renewing ability and dysregulated lineage specification hESCs. A unique composition BRG1-SWI/SNF hESCs was further defined by presence BAF250A, BAF170, BAF155, BAF53A, BAF47. Genome-wide expression analyses revealed...
The proper assembly and maturation of collagens necessitate the orchestrated hydroxylation glycosylation multiple lysyl residues in procollagen chains. Dysfunctions this multistep modification process can lead to severe collagen-associated diseases. To elucidate coordination processing activities, we determine cryo-EM structures enzyme complex formed by LH3/PLOD3 GLT25D1/ColGalT1, designated as KOGG complex. Our structural analysis reveals a tetrameric comprising dimeric LH3/PLOD3s...
Endoplasmic reticulum-associated degradation (ERAD) maintains protein homeostasis by retrieving misfolded proteins from the endoplasmic reticulum (ER) lumen into cytosol for degradation. The retrotranslocation of across ER membrane is an energy-consuming process, with detailed transportation mechanism still needing clarification. We determined cryo-EM structures hetero-decameric complex formed Derlin-1 tetramer and p97 hexamer. It showed intriguing asymmetric a putative coordinated squeezing...
Sodium excretion, a critical process in sodium homeostasis, occurs many tissues, including the kidney and intestine. Unlike kidney, hormonal regulation of intestinal excretion remains unclear. Atrial natriuretic peptide (ANP) is crucial hormone renal natriuresis. Corin protease for ANP activation. are expressed mainly heart. In this study, we investigated corin, ANP, receptor A (Npra) expression mouse intestines. was co-localized enteroendocrine cells, whereas Npra on luminal epithelial...