A5071087880- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- Viral Infections and Immunology Research
- Organ Transplantation Techniques and Outcomes
- Coronary Interventions and Diagnostics
- Cardiac Structural Anomalies and Repair
- Mechanical Circulatory Support Devices
- Polyomavirus and related diseases
- Cardiac Imaging and Diagnostics
- Cardiac Arrhythmias and Treatments
- Cardiac tumors and thrombi
- Organ and Tissue Transplantation Research
- Optical Coherence Tomography Applications
- Eosinophilic Disorders and Syndromes
- T-cell and B-cell Immunology
- Cardiac Ischemia and Reperfusion
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cardiac electrophysiology and arrhythmias
- MicroRNA in disease regulation
- Ultrasound and Hyperthermia Applications
- Infective Endocarditis Diagnosis and Management
- Peripheral Artery Disease Management
- Cardiomyopathy and Myosin Studies
Columbia University Irving Medical Center
2015-2025
Cornell University
2017-2025
Presbyterian Hospital
1989-2025
National Heart Lung and Blood Institute
2025
Columbia University
2014-2024
NewYork–Presbyterian Hospital
2002-2024
Texas Health Dallas
2015-2024
New York Hospital Queens
2000-2024
Digital Research Alliance of Canada
2024
Johns Hopkins University
2021
Rejection diagnosis by endomyocardial biopsy (EMB) is invasive, expensive and variable. We investigated gene expression profiling of peripheral blood mononuclear cells (PBMC) to discriminate ISHLT grade 0 rejection (quiescence) from moderate/severe (ISHLT > or = 3A). Patients were followed prospectively with sampling at post-transplant visits. Biopsies graded criteria locally three independent pathologists blinded clinical data. Known alloimmune pathways leukocyte microarrays identified 252...
Human tissue-resident memory T cells persist long term in transplanted lungs and develop from infiltrating recipient cells.
This study examined the effects of intracoronary irradiation on neointimal proliferation after overstretch balloon angioplasty in a normolipemic swine model restenosis.Restenosis percutaneous transluminal coronary represents, part, proliferative response vascular smooth muscle at site injury. We have previously shown that ionizing radiation, delivered by means an source, causes focal medial fibrosis. therefore hypothesized time might impair restenosis process.Nineteen juvenile underwent...
Endothelial cells have been shown to activate T cell responses alloantigens, triggering transplant rejection. However, they may also play a role in tolerance induction. Using RT–PCR we show here that alloantigen specific CD8+CD28− suppressor generated vitro are FOXP3 positive and interact with human endothelial cells. This interaction results the induction of inhibitory receptors down-regulation costimulatory adhesion molecules, thus rendering tolerogenic. In turn, tolerized elicit...
Cardiac transplantation, effective therapy for end-stage heart failure, is frequently complicated by allograft rejection, the mechanisms of which remain incompletely understood. Nitric oxide (NO), a vasodilator cytotoxic and negatively inotropic, can be produced in large amounts an inducible NO synthase (iNOS) response to cytokines. To investigate whether iNOS induced during cardiac hearts from Lewis or Wistar-Furth rats were transplanted into recipients. At day 5, allogeneic grafts...
The major threat to long-term survival of heart allograft recipients is the development graft atherosclerosis, which seems be a manifestation chronic rejection. To assess role anti-HLA antibodies in rejection we studied 107 patients and compared who formed with developed no antibodies. At 4 years actuarial was 90% nonproducer group 38% antibody-producers (P=0.038). We further explored possibility that HLA antigens from injured are released into circulation can found serum either free or...
In Brief Background There has been no large evaluation of the ISHLT 2004 acute cellular rejection grading scheme for heart graft endomyocardial biopsy specimens (EMBs). Methods We evaluated agreement within CARGO II pathology panel and between (acting by majority) collaborating centers (treated as a single entity), regarding grades 937 EMBs (with all ≥2R merged because small numbers). Results Overall all-grade was almost 71% both but, in cases, largely on grade 0: average pair pathologists,...
Allograft failure is common in lung-transplant recipients and leads to poor outcomes including early death. No reliable clinical tools exist identify patients at high risk for allograft failure. This study tested the use of donor-derived cell-free DNA (%ddcfDNA) as a sensitive marker graft injury predict impending failure.This multicenter, prospective cohort enrolled 106 subjects who underwent lung transplantation monitored them after development (defined severe chronic dysfunction [CLAD],...
Abstract Objectives Although diffuse alveolar damage, a subtype of acute lung injury (ALI), is the most common microscopic pattern in coronavirus disease 2019 (COVID-19), other pathologic patterns have been described. The aim study was to review autopsies from COVID-19 decedents evaluate spectrum pathology and correlate results with clinical, laboratory, radiologic findings. Methods A comprehensive quantitative 40 postmortem examinations performed. were categorized as follows: “major” when...
Background: After heart transplantation, endomyocardial biopsy (EMBx) is used to monitor for acute rejection (AR). Unfortunately, EMBx invasive, and its conventional histological interpretation has limitations. This a validation study assess the performance of sensitive blood biomarker—percent donor-derived cell-free DNA (%ddcfDNA)—for detection AR in cardiac transplant recipients. Methods: multicenter, prospective cohort recruited subjects collected plasma samples contemporaneously with...
Decisions to transplant organs from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid test-positive (NAT+) donors must balance risk of donor-derived transmission events (DDTE) with the scarcity available organs.Organ Procurement and Transplantation Network (OPTN) data were used compare organ utilization recipient outcomes between SARS-CoV-2 NAT+ NAT- donors. was defined by either a positive upper or lower tract (LRT) sample within 21 days procurement. Potential DDTE...
Rationale: The association of acute cellular rejection (ACR) with chronic lung allograft dysfunction (CLAD) in transplant recipients has primarily been described before consensus recommendations incorporating restrictive phenotypes. Furthermore, the degree molecular injury during ACR CLAD or death remains undefined. Objectives: To investigate risk and to further if this depends on injury. Methods: This multicenter, prospective cohort study included 188 recipients. Subjects underwent serial...
The goal of this study was to define the regulation nitric oxide release by coronary microvessels from failing and nonfailing human heart determine role local kinin production in elaboration microvascular endothelium.Ten hearts humans with end-stage failure two patients without were harvested at time orthotopic cardiac transplantation. Microvessels sieved nitrite determined Griess reaction. incubated presence agonists for (acetylcholine bradykinin), which caused dose-dependent increases...
Osteopontin (OP) has been identified in cultured rat cardiac fibroblasts, where it contributes to angiotensin II (AII)-induced remodeling processes; cardiomyocytes; and macrophages tissues with inflammation. However, the presence of OP not reported histological sections myocardial tissue. In present study, we investigated (1) regulation mRNA expression (2) localization neonatal adult normal hypertrophied hearts; (3) histology specimens from humans either myocyte hypertrophy or no...