A5060533680- Retinoids in leukemia and cellular processes
- DNA Repair Mechanisms
- interferon and immune responses
- Acute Myeloid Leukemia Research
- Marine Bivalve and Aquaculture Studies
- Genomics and Chromatin Dynamics
- Aquaculture disease management and microbiota
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Virus-based gene therapy research
- bioluminescence and chemiluminescence research
- Vibrio bacteria research studies
- Fungal and yeast genetics research
- Estrogen and related hormone effects
- Antioxidant Activity and Oxidative Stress
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Physiological and biochemical adaptations
- Methane Hydrates and Related Phenomena
- Identification and Quantification in Food
- Parvovirus B19 Infection Studies
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- HIV Research and Treatment
Centre National de la Recherche Scientifique
2009-2025
Laboratoire des Sciences de l'Environnement Marin
2007-2012
Institut Universitaire Européen de la Mer
2007-2012
Université Paris Cité
1998-2010
La Ligue Contre le Cancer
1998-2010
Inserm
1998-2010
Université de Bretagne Occidentale
2007-2009
Station Biologique de Roscoff
2004-2005
Hôpital Saint-Louis
1994-2001
Shanghai Institute of Hematology
1997-1998
Promyelocytic leukemia (PML) is the organizer of nuclear matrix domains, PML bodies (NBs), with a proposed role in apoptosis control. In acute promyelocytic leukemia, PML/retinoic acid receptor (RAR) α expression disrupts NBs, but therapies such as retinoic or arsenic trioxide (As2O3) restore them. conjugated by ubiquitin-related peptide SUMO-1, process enhanced As2O3 and to target matrix. We demonstrate that triggers proteasome-dependent degradation PML/RARα this requires specific...
Acute promyelocytic leukemia (APL) is associated with the t(15;17) translocation, which generates a PML/RARα fusion protein between PML, growth suppressor localized on nuclear matrix-associated bodies, and RARα, receptor for retinoic acid (RA). was proposed to block myeloid differentiation through inhibition of response, as does dominant negative RARα mutant. In addition, in APL cells, displaces PML other body (NB) antigens onto microspeckles, likely resulting loss and/or NB functions. RA...
(R)-Roscovitine (CYC202) is often referred to as a "selective inhibitor of cyclin-dependent kinases." Besides its use biological tool in cell cycle, neuronal functions, and apoptosis studies, it currently evaluated potential drug treat cancers, neurodegenerative diseases, viral infections, glomerulonephritis. We have investigated the selectivity (R)-roscovitine using three different methods: 1) testing on wide panel purified kinases that, along with previously published data, now reaches 151...
Analyzing the pathways by which retinoic acid (RA) induces promyelocytic leukemia/retinoic receptor α (PML/RAR ) catabolism in acute leukemia (APL), we found that, addition to caspase-mediated PML/RAR cleavage, RA triggers degradation of both and RAR . Similarly, non-APL cells, directly targeted fusions proteasome pathway. Activation either or RXR specific agonists induced proteins. Conversely, a mutation that abolishes heterodimer formation DNA binding, blocked degradation. Mutations...
Poly(ADP-ribose) polymerase (EC 2.4.2.30) is a zinc-binding protein that specifically binds to DNA strand break in zinc-dependent manner. We describe here the cloning and expression Escherichia coli of cDNA fragment encoding two putative zinc fingers (FI FII) domain human poly(ADP-ribose) polymerase. Using site-directed mutagenesis, we identified amino acids involved metal coordination analyzed consequence altering proposed zinc-finger structures on binding. Disruption binding ability second...
The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human homologs, designated HHR6A HHR6B. 152-amino acid proteins share 95% sequence identity with each other approximately 70% 85% overall homologs from yeasts (S. Schizosaccharomyces pombe) Drosophila melanogaster, respectively. Neither possess acidic C-terminal present in S. protein. Genetic complementation...
The interferon (IFN)-induced promyelocytic leukemia (PML) protein is specifically associated with nuclear bodies (NBs) whose functions are yet unknown. Two of the NB-associated proteins, PML and Sp100, induced by IFN. Here we show that overexpression not Sp100 induces resistance to infections vesicular stomatitis virus (VSV) (a rhabdovirus) influenza A (an orthomyxovirus) but encephalomyocarditis picornavirus). Inhibition viral multiplication was dependent on both level expression...
Abstract Since 1998, Haliotis tuberculata mass mortalities have been occurring regularly in wild abalone populations France during their reproductive period and conjunction with seawater summer temperature maxima Vibrio harveyi presence. To confirm the importance of bacterial exposure, status on susceptibility, experimental infections via bath exposure were performed using ranging from immature to reproductively mature. Ripe more susceptible bacterium than specimens ( P <0.001), a...
Xeroderma pigmentosum (XP) patients are extremely sensitive to ultraviolet (UV) light and suffer from a high incidence of skin cancers, due defect in nucleotide excision repair. The disease is genetically heterogeneous, seven complementation groups, A-G, have been identified. Homologs human repair genes ERCC1, XPDC/ERCC2, XPAC identified the yeast Saccharomyces cerevisiae. Since no homolog XPBC/ERCC3 existed among known genes, we cloned by using XPBC cDNA as hybridization probe. homolog,...
In acute promyelocytic leukemia (APL), the typical t(15;17) and rare t(11;17) translocations express, respectively, PML/RARα PLZF/RARα fusion proteins (where RARα is retinoic acid receptor α). Herein, we demonstrate that PLZF PML interact with each other colocalize onto nuclear bodies (NBs). Furthermore, induction of expression by interferons leads to a recruitment NBs without increase in levels protein. localize same microspeckled domains appear be common targets for two APL. Although does...