A5100751132- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Retinoids in leukemia and cellular processes
- Acute Myeloid Leukemia Research
- Plant and animal studies
- Protein Degradation and Inhibitors
- Plant tissue culture and regeneration
- RNA Research and Splicing
- DNA Repair Mechanisms
- Drug-Induced Ocular Toxicity
- Lipid metabolism and biosynthesis
- Polysaccharides and Plant Cell Walls
- Microtubule and mitosis dynamics
- Zebrafish Biomedical Research Applications
- Plant biochemistry and biosynthesis
- Remote Sensing and Land Use
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Reproductive System and Pregnancy
- Antioxidant Activity and Oxidative Stress
- Fatty Acid Research and Health
- Bacterial biofilms and quorum sensing
- Plant Physiology and Cultivation Studies
- Genomics and Chromatin Dynamics
Shanghai Normal University
2016-2025
Chongqing Medical University
2024
Peking University
2024
Peking University Cancer Hospital
2024
Shanghai Jiao Tong University
2010-2021
Hôpital Saint-Louis
2021
Anhui University
2016
Zhejiang University
2011-2015
Délégation Paris 7
2014
Sorbonne Paris Cité
2014
Summary Downregulation of the transcription factor AtMYB103 using transgenic technology results in early tapetal degeneration and pollen aberration during anther development Arabidopsis thaliana . This paper describes functional analysis gene three knock‐out mutants. Two male sterile mutants, ms188‐1 ms188‐2 , were generated by ethyl‐methane sulfonate (EMS) mutagenesis. A map‐based cloning approach was used, ms188 mapped to a 95.8‐kb region on chromosome 5 containing an factor. Sequence...
Summary In Arabidopsis, the tapetum plays important roles in anther development by providing enzymes for callose dissolution and materials pollen‐wall formation, supplying nutrients pollen development. Here, we report identification characterization of a male‐sterile mutant, defective tapetal function 1 ( tdf1 ), that exhibits irregular division dysfunction tapetum. The TDF1 gene was characterized using map‐based cloning strategy, confirmed genetic complementation. It encodes putative R2R3...
Analyzing the pathways by which retinoic acid (RA) induces promyelocytic leukemia/retinoic receptor α (PML/RAR ) catabolism in acute leukemia (APL), we found that, addition to caspase-mediated PML/RAR cleavage, RA triggers degradation of both and RAR . Similarly, non-APL cells, directly targeted fusions proteasome pathway. Activation either or RXR specific agonists induced proteins. Conversely, a mutation that abolishes heterodimer formation DNA binding, blocked degradation. Mutations...
Abstract During microsporogenesis, the microsporocyte (or microspore) plasma membrane plays multiple roles in pollen wall development, including callose secretion, primexine deposition, and exine pattern determination. However, proteins that participate these processes are still not well known. Here, we report a new gene, RUPTURED POLLEN GRAIN1 (RPG1), encodes protein is required for formation of microspores Arabidopsis (Arabidopsis thaliana). The rpg1 mutant exhibits severely reduced male...
In anther development, tapetal cells take part in complex processes, including endomitosis and apoptosis (programmed cell death). The tapetum provides many of the proteins, lipids, polysaccharides other molecules necessary for pollen development. Several transcription factors, DYT1, TDF1, AMS, MS188 MS1, have been reported to be essential development function Arabidopsis thaliana. Here, we present a detailed cytological analysis knockout mutants these genes, along with an situ RNA...
The tapetum plays a critical role during the development and maturation of microspores. DYSFUNCTIONAL TAPETUM 1 (DYT1) is essential for early tapetal development. Here, we determined that promoter region (-550 to -463 bp) contains indispensable cis-elements DYT1 expression. Although transcripts can be detected in both meiocytes cells, localization DYT1-GFP demonstrated strictly located cells microsporogenesis. Chromatin immunoprecipitation (ChIP) analysis revealed directly binds Defective...
Abstract The pollen wall, an essential structure for function, consists of two layers, inner intine and outer exine. latter is further divided into sexine nexine. Many genes involved in development have been reported, which the MYB transcription factor Male Sterile 188 (MS188) specifies Arabidopsis . However, nexine formation remains poorly understood. Here we report knockout TRANSPOSABLE ELEMENT SILENCING VIA AT-HOOK ( TEK) leads to absence TEK encodes AT-hook nuclear localized family...
Abstract In angiosperms, pollen wall pattern formation is determined by primexine deposition on the microspores. Here, we show that AUXIN RESPONSE FACTOR17 (ARF17) essential for and development in Arabidopsis (Arabidopsis thaliana). The arf17 mutant exhibited a male-sterile phenotype with normal vegetative growth. ARF17 was expressed microsporocytes microgametophytes from meiosis to bicellular microspore stage. Transmission electron microscopy analysis showed absent mutant, which leads...
Sporopollenin is the major component of outer pollen wall (sexine). It synthesized using a pathway approximately eight genes in Arabidopsis (Arabidopsis thaliana). MALE STERILITY188 (MS188) and its direct upstream regulator ABORTED MICROSPORES (AMS) are two transcription factors essential for tapetum development. Here, we show that all sporopollenin biosynthesis proteins specifically expressed secreted into anther locules. MS188, MYB factor tapetum, directly regulates expression POLYKETIDE...
Tapetum development and pollen production are regulated by a complex transcriptional network that consists of group tapetum-specific Arabidopsis transcription factors (TFs). Among these TFs, DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1 (TDF1) encodes an R2R3 MYB factor, ABORTED MICROSPORE (AMS) basic helix-loop-helix (bHLH) factor. However, knowledge regarding the regulatory role TDF1 in anther remains limited. Here, we discovered directly regulates AMS via AACCT cis-element. We found...
Abstract The major antioxidant L-ascorbic acid (AsA) plays important roles in plant growth, development, and stress responses. However, the importance of AsA concentration regulation metabolism reproduction remain unclear. In Arabidopsis (Arabidopsis thaliana) anthers, tapetum monolayer undergoes cell differentiation to support pollen development. Here, we report that a transcription factor, DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1 (TDF1), inhibits tapetal division leading...
Summary P/TGMS (Photo/thermo‐sensitive genic male sterile) lines are crucial resources for two‐line hybrid rice breeding. Previous studies revealed that slow development is a general mechanism sterility–fertility conversion of in Arabidopsis. However, the difference genes between and Arabidopsis suggests presence distinct rice. In this study, we isolated novel line, ostms19 , which shows sterility under high‐temperature conditions fertility low‐temperature conditions. OsTMS19 encodes...
Abstract Acetyl-CoA is the main substrate of lipid metabolism and functions as an energy source for plant development. In cytoplasm, acetyl-CoA mainly produced by ATP-citrate lyase (ACL), which composed ACLA ACLB subunits. this study, we isolated restorer-4 (res4) thermosensitive genic male sterile mutant reversible sterile-2 (rvms-2) in Arabidopsis (Arabidopsis thaliana). RES4 encodes ACLB1, res4 harbors a point mutation (Gly584 to Arg) citryl-CoA domain. Both subunits are expressed tapetal...
(1) Background: Endophytic fungi play an important role in plant growth and stress resistance. The presence of a special fungal taxon such as the dark septate endophytic (DSE) alpine environments is particularly for resistance to environmental stresses. However, composition root different between host plants has not been well studied. (2) Results: A total 408 culturable were isolated from roots Saussurea involucrata Rhodiola crenulata which collected 5 plots Tianshan Karakoram Mountains...
The sexine layer of pollen grain is mainly composed sporopollenins. sporophytic secretory tapetum required for the biosynthesis sporopollenin. Although several enzymes involved in sporopollenin have been reported, regulatory mechanism these tapetal remains elusive. ABORTED MICROSPORES (AMS) and MALE STERILE 188/MYB103/MYB80 (MS188/MYB103/MYB80) are two cell-specific transcription factors wall formation. AMS functions upstream MS188. Here we report that MS188 target CYP703A2 gene, which...
In flowering plants, male gametophyte development occurs in the anther. Tapetum, innermost of four anther somatic layers, surrounds developing reproductive cells to provide materials for pollen development. A genetic pathway DYT1-TDF1-AMS-MS188 regulating tapetum has been proven. Here we used laser microdissection and pressure catapulting capture analyze transcriptome data Arabidopsis at two stages. With a comprehensive analysis by microarray dyt1, tdf1, ams, ms188 mutants, identified...
Abstract Arabidopsis thaliana CYCLIN-DEPEDENT KINASE G1 (CDKG1) belongs to the family of cyclin-dependent protein kinases that were originally characterized as cell cycle regulators in eukaryotes. Here, we report CDKG1 regulates pre-mRNA splicing CALLOSE SYNTHASE5 (CalS5) and, therefore, pollen wall formation. The knockout mutant cdkg1 exhibits reduced male fertility with impaired callose synthesis and abnormal sixth intron CalS5 pre-mRNA, a rare type GC 5′ splice site, is abnormally spliced...
Abstract Sporophytic pollen coat proteins (sPCPs) derived from the anther tapetum are deposited into wall cavities and function in pollen–stigma interactions, hydration, environmental protection. In Arabidopsis, 13 highly abundant have been identified coat, including seven major glycine-rich GRP14, 16, 17, 18, 19, 20, GRP–oleosin; two caleosin-related family (AT1G23240 AT1G23250); three lipase EXL4, EXL5 EXL6, ATA27/BGLU20. Here, we show that EXL4 EXL6 fused with green fluorescent protein...