A5089335621- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Poxvirus research and outbreaks
- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- Mosquito-borne diseases and control
- Hepatitis B Virus Studies
- Asthma and respiratory diseases
- interferon and immune responses
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Animal Virus Infections Studies
- Transgenic Plants and Applications
- Hepatitis Viruses Studies and Epidemiology
- Leptospirosis research and findings
Simon Fraser University
2016-2025
Faculty of Public Health
2022-2025
University of British Columbia
2016
Agricultural Research Service
1996-2010
Michigan State University
2003-2010
Michigan Department of Agriculture and Rural Development
2007-2010
United States Department of Agriculture
2010
National Institutes of Health
2001-2006
National Institute of Infectious Diseases
1998-2005
The University of Tokyo
1992-2003
Abstract Humoral responses to COVID-19 vaccines in people living with HIV (PLWH) remain incompletely characterized. We measured circulating antibodies against the SARS-CoV-2 spike protein receptor-binding domain (RBD), ACE2 displacement and viral neutralization activities one month following first second vaccine doses, again 3 months dose, 100 adult PLWH 152 controls. All were receiving suppressive antiretroviral therapy, median CD4+ T-cell counts of 710 (IQR 525–935) cells/mm , though nadir...
Adding to the Antiviral Arsenal The envelope of influenza virus contains two immunodominant glycoproteins: hemagglutinin and neuraminidase (NA). Existing antivirals like zanamivir (Relenza) oseltamivir (Tamiflu) target NA; however, development drug resistance is a problem. Kim et al. (p. 71 , published online 21 February) now report different class NA inhibitors. catalyzes removal sialic acids from surface host cells initiate entry. Discovery NA–sialic acid intermediate led production...
Abstract Background The magnitude and durability of immune responses to coronavirus disease 2019 (COVID-19) mRNA vaccines remain incompletely characterized in the elderly. Methods Anti-spike receptor-binding domain (RBD) antibodies, angiotensin-converting enzyme 2 (ACE2) competition, virus neutralizing activities were assessed plasma from 151 health care workers older adults (range, 24–98 years age) 1 month following first vaccine dose, 3 months second dose. Results Older exhibited...
Third coronavirus disease 2019 (COVID-19) vaccine doses are broadly recommended, but immunogenicity data remain limited, particularly in older adults.We measured circulating antibodies against the severe acute respiratory syndrome 2 (SARS-CoV-2) spike protein receptor-binding domain, ACE2 displacement, and virus neutralization ancestral omicron (BA.1) strains from prevaccine up to 1 month following third dose, 151 adults aged 24-98 years who received COVID-19 mRNA vaccines.Following doses,...
Abstract Background Longer-term humoral responses to 2-dose coronavirus disease 2019 (COVID-19) vaccines remain incompletely characterized in people living with human immunodeficiency virus (HIV) (PLWH), as do initial a third dose. Methods We measured antibodies against the severe acute respiratory syndrome 2 (SARS-CoV-2) spike protein receptor-binding domain, angiotensin-converting enzyme (ACE2) displacement, and viral neutralization wild-type Omicron strains up 6 months after vaccination,...
SARS-CoV-2 Omicron subvariants with increased transmissibility and immune evasion are spreading globally alarming persistence. Whether the mutations evolution of spike (S) alter viral hijacking human TMPRSS2 for entry remains to be elucidated. This is particularly important investigate because large number diversity S reported since emergence BA.1. Here we report that a molecular determinant all clinical isolates tested in lung cells, including ancestral (BA.1, BA.2, BA.5), contemporary...
ABSTRACT The full-length nucleoprotein of Crimean-Congo hemorrhagic fever virus (CCHFV; 482 amino acid residues) was expressed as a His-tagged recombinant protein (His-CCHFV rNP) in the baculovirus system. His-CCHFV rNP efficiently insect cells and purified by Ni 2+ column chromatography. Using this substrate, an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) developed. We evaluated sensitivity specificity IgG ELISA, using serum samples previously determined to be antibody...
With the increase in international traffic, risk of introducing rare but severe infectious diseases like Ebola hemorrhagic fever is increasing all over world. However, system for diagnosis virus infection available a limited number countries. In present study, we developed an antigen-detection enzyme-linked immunosorbent assay (ELISA) using novel monoclonal antibody (MAb) to nucleoprotein (NP). This recognized epitope defined by 26-amino-acid stretch near C terminus NP. sandwich ELISA with...
A HeLa cell line continuously expressing recombinant nucleoprotein (rNP) of the Crimean-Congo hemorrhagic fever virus (CCHFV) was established by transfection with an expression vector containing cDNA CCHFV NP (pKS336-CCHFV-NP). These cells were used as antigens for indirect immunofluorescence (IF) to detect immunoglobulin G antibodies CCHFV. The sensitivity and specificity this IF technique examined using serum samples compared those CCHFV-infected Vero E6 (authentic antigen). Staining rNP...
ABSTRACT The full-length nucleoprotein (NP) of Ebola virus (EBO) was expressed as a His-tagged recombinant protein (His-EBO-NP) by baculovirus system. Carboxy-terminal halves NPs EBO and Marburg (MBG) were glutathione S -transferase-tagged proteins in an Escherichia coli antigenic regions on the MBG determined both Western blotting enzyme-linked immunosorbent assay (ELISA) to be located C-terminal halves. 110 102 amino acids MBG, respectively, possess strong antigenicity. NP strongly insect...
The Marek's disease virus (MDV) induces T-cell tumors in susceptible chickens. Of the 80 to 100 known MDV genes, only MEQ gene was shown have transforming properties. Further evidence that is probably principal oncogene came when researchers used overlapping cosmid clones of and demonstrated deleting resulted a highly protective (MD) vaccine. We deleted both copies from bacterial artificial chromosome clone (BAC) MDV. virus, BACdelMEQ, completely attenuated did not appear any adverse effect...
Limited data exist regarding the immune benefits of fourth COVID-19 vaccine doses in people with HIV (PWH) receiving antiretroviral therapy (ART), particularly now that most have experienced a SARS-CoV-2 infection. We quantified wild-type, Omicron-BA.5 and Omicron-BQ.1-specific neutralization up to 1 month post-fourth dose 63 (19 SARS-CoV-2-naive 44 SARS-CoV-2-experienced) PWH.A longitudinal observational cohort.Quantification wild-type-, Omicron-BA.5, using live virus assays.Participants...
Genetic homogeneity of a test population is essential to precisely associate viral genome sequence and its phenotype at the nucleotide level. However, not easy achieve for Marek's disease virus (MDV) due strictly cell-associated replication. To address this problem, two virulent infectious bacterial artificial chromosome (BAC) clones MDV were generated from an previously cloned as five overlapping cosmids. The Md5SN5BAC clone has BAC vector inserted between 3′ ends UL3 UL4, such that no...
Abstract We have previously reported a potent neuraminidase inhibitor that comprises carbocyclic analogue of zanamivir in which the hydrophilic glycerol side chain is replaced by hydrophobic 3‐pentyloxy group oseltamivir. This hybrid showed excellent inhibitory properties inhibition assay ( K i =0.46 n M ; (zanamivir) =0.16 ) and viral replication cell culture at 10 −8 . As part this lead optimization, we now report novel spirolactam shows comparable activity to our compound −7 The was...
Abstract The SARS-CoV-2 pandemic has highlighted the weaknesses of relying on single-use mask and respirator personal protective equipment (PPE) global supply chain that supports this market. There have been no major innovations in filter technology for PPE past two decades. Non-woven textiles used filtering are products healthcare environment; use protection is focused preventing infection from airborne or aerosolized pathogens such as Influenza A virus SARS-CoV-2. Recently, C–H bond...
ABSTRACT Background Longer-term humoral responses to two-dose COVID-19 vaccines remain incompletely characterized in people living with HIV (PLWH), as do initial a third dose. Methods We measured antibodies against the SARS-CoV-2 spike protein receptor-binding domain, ACE2 displacement and viral neutralization wild-type Omicron strains up six months following vaccination, one month dose, 99 PLWH receiving suppressive antiretroviral therapy, 152 controls. Results Though naturally decline we...
Background: Limited data exist regarding longer term antibody responses following three-dose coronavirus disease 2019 (COVID-19) vaccination, and the impact of a first SARS-CoV-2 infection during this time, in people with HIV (PWH) receiving suppressive antiretroviral therapy (ART). We quantified wild-type-specific, Omicron BA.1-specific BA.5-specific up to 6 months post-third dose 64 PWH 117 controls who remained COVID-19-naive or experienced their time. Design: Longitudinal observational...
SARS-CoV-2, the causative virus of COVID-19, continues to threaten global public health. COVID-19 is a multi-organ disease, causing not only respiratory distress, but also extrapulmonary manifestations, including gastrointestinal symptoms with SARS-CoV-2 RNA shedding in stool long after clearance. Despite vaccination and existing antiviral treatments, variants concern are still emerging circulating. Of note, new Omicron BA.5 sublineages both increasingly evade neutralizing antibodies...
Antivirals with broad coronavirus activity are important for treating high-risk individuals exposed to the constantly evolving SARS-CoV-2 variants of concern (VOCs) as well emerging drug-resistant variants. We developed and characterized a novel class active-site-directed 3-chymotrypsin-like protease (3CLpro) inhibitors (C2-C5a). Our lead direct-acting antiviral (DAA), C5a, is non-covalent, non-peptide dissociation constant 170 nM against recombinant 3CLpro. The compounds C2-C5a exhibit...
Marek’s disease virus (MDV) is a naturally occurring oncogenic avian herpesvirus that causes neurological disorders and T cell lymphoma in domestic chickens. Identification functional characterization of the individual factors involved (MD) resistance or pathogenesis will enhance our understanding MDV further genetic improvement To study basis for to MD, strategy combined protein-protein interaction screens followed by linkage analysis was performed. The protein US10 used as bait an...