A5033312405- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV/AIDS Research and Interventions
- Autophagy in Disease and Therapy
- Cancer Cells and Metastasis
- IL-33, ST2, and ILC Pathways
- vaccines and immunoinformatics approaches
- Cannabis and Cannabinoid Research
- T-cell and B-cell Immunology
- Sex work and related issues
- Eosinophilic Esophagitis
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Cell Image Analysis Techniques
- Mosquito-borne diseases and control
- Immune Response and Inflammation
- 3D Printing in Biomedical Research
- Reproductive System and Pregnancy
- Calcium signaling and nucleotide metabolism
- Microscopic Colitis
- Inflammatory Bowel Disease
- Berberine and alkaloids research
Emma Kinderziekenhuis
2017-2024
University of Amsterdam
2017-2024
Amsterdam University Medical Centers
2019-2024
Vrije Universiteit Amsterdam
2024
Amsterdam UMC Location University of Amsterdam
2017
Abstract Innate lymphoid cells (ILC), including natural killer (NK) cells, are implicated in host-defense and tissue-growth. However, the composition kinetics of NK intestine during first year life, when infants broadly exposed to exogenous antigens, still unclear. Here we show that CD103 + major ILC population small intestines infants. When compared adult intestinal infant exhibit a robust effector phenotype, characterized by Eomes, perforin granzyme B expression, superior degranulation...
Crosstalk between immune cells and intestinal stem (ISCs) in vivo plays a critical role tissue homeostasis inflammation; however, vitro models based on primary recapitulating this interaction were lacking. Here, we provide detailed protocol for an autologous long-term 3D co-culture system of human CD4+ T ISCs to study cell-intestinal epithelial cell interactions during development inflammation. For complete details the use execution protocol, please refer Schreurs et al. (2019).
Abstract Chronic HIV-1 infection is characterized by T-cell dysregulation that partly restored antiretroviral therapy. Autophagy a critical regulator of function. Here, we demonstrate protective role for autophagy in disease pathogenesis. Targeted analysis genetic variation core gene ATG16L1 reveals the previously unidentified rs6861 polymorphism, which correlates functionally with enhanced and clinically improved survival untreated HIV-1-infected individuals. T-cells carrying rs6861(TT)...
SARS-CoV-2, the causative virus of COVID-19, continues to threaten global public health. COVID-19 is a multi-organ disease, causing not only respiratory distress, but also extrapulmonary manifestations, including gastrointestinal symptoms with SARS-CoV-2 RNA shedding in stool long after clearance. Despite vaccination and existing antiviral treatments, variants concern are still emerging circulating. Of note, new Omicron BA.5 sublineages both increasingly evade neutralizing antibodies...
Dengue virus (DENV), transmitted by infected mosquitoes, is a major public health concern, with approximately half the world’s population at risk for infection. Recent decades have increasing incidence of dengue-associated disease alongside growing frequency outbreaks. Although promising progress has been made in anti-DENV immunizations, post-infection treatment remains limited to non-specific supportive treatments. Development antiviral therapeutics thus required limit DENV dissemination...
Abstract Current direct-acting antiviral therapies are highly effective in suppressing HIV-1 replication. However, mucosal inflammation undermines prophylactic treatment efficacy, and persists long-lived tissue-derived dendritic cells (DCs) CD4 + T of treated patients. Host-directed strategies an emerging therapeutic approach to improve therapy outcomes infectious diseases. Autophagy functions as innate mechanism by degrading viruses specialized vesicles. Here, we investigated the impact...
Early life is characterized by extraordinary challenges, including rapid tissue growth and immune adaptation to foreign antigens after birth. During this developmental stage, infants have an increased risk of immune-mediated diseases. Here, we demonstrate that tissue-resident, interleukin (IL)-13- IL-4-producing group 2 innate lymphoid cells (ILC2s) are enriched in human infant intestines compared adult intestines. Organoid systems were employed assess the role intestinal ILC2s development...
Studies on immune cells derived from the human intestine are needed to understand pathogenesis of gastrointestinal diseases and develop novel treatment strategies. Isolation techniques extract these intestinal tissue largely based murine studies comparative data isolation is scarce. In this study we evaluated cell yield, viability, surface-molecule expression mononuclear leukocytes, comparing three obtain a single suspension intestine; low concentrations either enzymes Collagenase D or...
Gastrointestinal viral infections are a major global cause of disease and mortality in infants. Cytotoxic CD8+ T cells critical to achieve control. However, studies investigating the development cell immunity human tissues early life lacking. Here, we investigated maturation compartment fetal, infant adult intestinal tissues. exhibiting memory phenotype were already detected fetal intestines increased after birth. Infant preferentially harbored effector CCR7-CD45RA-CD127-KLRG1+/- compared...
Identification of immunogenic cancer neoantigens as targets for therapy is challenging. Here, we integrate the whole-genome and long-read transcript sequencing cancers to identify collection neo-open reading frame peptides (NOP) expressed in tumors. We termed this NOPs tumor framome. represent tumor-specific that are different from wild-type proteins may be strongly immunogenic. describe a class hidden derive structural genomic variants involving an upstream protein coding gene driving...
Abstract Introduction The significant rise in incidence of Hepatitis C virus ( HCV ) infection among men‐who‐have‐sex‐with‐men MSM living with HIV ‐1 suggests that under specific circumstances is transmitted via sexual contact. During transmission has to cross the epithelial barrier either directly enter blood stream or indirectly mucosal immune cells. However, mechanisms remain unclear. We investigated role Langerhans cells LC s) susceptibility during contact as s are first tissues...
Introduction Appendicitis is one of the most common causes acute abdominal surgery in children. The clinical course appendicitis ranges from simple to complex appendicitis. mechanisms underlying heterogeneity children remain largely unclear. Dysregulated T cell responses play an important role several inflammatory diseases intestine, but extend dysregulation less well known. Methods To characterize appendiceal cells and we performed in-depth immunophenotyping appendiceal-derived by flow...
The gastrointestinal tract is a prominent portal of entry for HIV-1 during sexual or perinatal transmission, as well major site persistence and replication. Elucidation underlying mechanisms intestinal infection are thus needed the advancement curative therapies. Here, we present human 2D immuno-organoid system to model disease that recapitulates tissue compartmentalization epithelial-immune cellular interactions. Our data demonstrate apical exposure epithelium results in viral...
<p>Figure S10: A) NOP expression compared to normal genes.</p>
<p>Figure S15: Tetramer gating strategy.</p>
<p>Figure S7: Protein mass spectrometry results for A375 NOPs.</p>
<p>Figure S16: Expansion of FRM-specific CD8T cells after priming with relevant peptide in PBMC from healthy individuals.</p>
<p>Figure S14: A375 immunopeptidomics.</p>
<p>Figure S1: RNA-guided tumor genome reconstruction.</p>
<p>Figure S4: Overview of somatic mutation statistics for tumor samples analyzed by WGS in this study.</p>
<p>Figure S16: Expansion of FRM-specific CD8T cells after priming with relevant peptide in PBMC from healthy individuals.</p>
<p>Figure S8: Comparison of EasyFuse short-read fusion gene caller with long-read and WGS guided NOP identification.</p>
<p>Figure S11: Example of a hidden NOP resulting from complex chromosomal rearrangement in tumor sample LUN022.</p>