A5017947859- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Additive Manufacturing and 3D Printing Technologies
- Corneal Surgery and Treatments
- Cellular Mechanics and Interactions
- biodegradable polymer synthesis and properties
- Graphene and Nanomaterials Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Ocular Surface and Contact Lens
- Dental Implant Techniques and Outcomes
- Mesenchymal stem cell research
- Cell Image Analysis Techniques
- Computational Drug Discovery Methods
- Cancer, Hypoxia, and Metabolism
- Orthopaedic implants and arthroplasty
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Drug Delivery Systems
- Periodontal Regeneration and Treatments
- Corneal surgery and disorders
- Nanoplatforms for cancer theranostics
- Hydrogels: synthesis, properties, applications
- Antimicrobial Peptides and Activities
- Cancer Cells and Metastasis
University of Nottingham
2016-2025
Park University
2021
Translational Research Institute
2014
The University of Queensland
2014
Mater Research
2014
University Children's Hospital Zurich
2012
Amsterdam UMC Location Vrije Universiteit Amsterdam
2012
Seoul National University Hospital
2012
Seoul National University
2012
Cancer Center Amsterdam
2012
Conceptual models of carcinogenesis typically consist an evolutionary sequence heritable changes in genes controlling proliferation, apoptosis, and senescence. We propose that these steps are necessary but not sufficient to produce invasive breast cancer because intraductal tumour growth is also constrained by hypoxia acidosis develop as cells proliferate into the lumen away from underlying vessels. This requires evolution glycolytic acid-resistant phenotypes that, we hypothesise, critical...
Mast cell localization within the airway smooth muscle (ASM)-bundle plays an important role in development of hyper-responsiveness (AHR). Genomewide association studies implicate 'alarmin' IL-33 asthma, but its mast cell-ASM interactions is unknown.We examined expression and functional bronchial biopsies patients with without ex vivo ASM, cells, cocultured cells a mouse model system.IL-33 protein was assessed human tissue from 9 healthy controls, 18 mild-to-moderate 12 severe asthmatic by...
Nearly 30% of women with early-stage breast cancer develop recurrent disease attributed to resistance systemic therapy. Prevailing models chemotherapy failure describe three resistant phenotypes: cells alterations in transmembrane drug transport, increased detoxification and repair pathways, leading apoptosis. Proliferative activity correlates tumor sensitivity. Cell-cycle status, controlling proliferation, depends on local concentration oxygen nutrients. Although physiologic due diffusion...
Selective laser melting (SLM) has previously been shown to be a viable method for fabricating biomedical implants; however, the surface chemistry of SLM fabricated parts is poorly understood. In this study, X-ray photoelectron spectroscopy (XPS) was used determine chemistries (a) as-fabricated (SLM-AF) Ti6Al4V and (b) mechanically polished (SLM-MP) samples compared with (c) traditionally manufactured (forged) samples. The SLM–AF observed porous an average roughness (Ra) 17.6 ± 3.7 μm. SLM-AF...
In the human gastrointestinal tract, microorganisms are present in large numbers colon but sparse proximal small intestine. this study, we have shown that acid extracts of fresh terminal ileal mucosal samples mediate antimicrobial activity. Following cation-exchange chromatography, one eluted fractions demonstrated antibacterial activity against bacteria normally resident colonic lumen. This was further fractionated by reverse-phase high-performance liquid chromatography and identified as...
Ro 32‐3555 (3( R )‐(cyclopentylmethyl) ‐ 2( ) [(3,4,4 trimethyl‐2,5‐dioxo‐1‐imidazolidinyl)methyl] 4‐oxo‐4‐piperidinobutyrohydroxamic acid) is a potent, competitive inhibitor of human collagenases 1, 2 and 3 ( K i values 3.0, 4.4 3.4 n M , respectively). The compound selective over the related matrix metalloproteinases stromelysin gelatinases A B 527, 154 59 inhibited interleukin‐1α (IL‐1α)‐induced cartilage collagen degradation in vitro bovine nasal explants (IC 50 =60 ). was well absorbed...
Orthogonal wettability and topographical gradients in a combinatorial sample format are fabricated using plasma-polymer-coated microgrooved surfaces. Preferred cell proliferation is found on specific combinations of topography chemistry. This proof-of-concept study demonstrates the potential applications this for investigating relationship between multiple surface properties cellular response high-throughput manner.
Sustained and controlled delivery of growth factors, such as bone morphogenetic protein 2 (BMP-2), from polymer scaffolds has excellent potential for enhancing regeneration. The present study investigated the use novel sintered prepared using temperature-sensitive PLGA/PEG particles. Growth factors can be incorporated into these by mixing reconstituted factor with particles prior to sintering. ability deliver BMP-2 in a sustained manner was assessed osteogenic determined mouse calvarial...
The current study has investigated the use of decellularised, demineralised bone extracellular matrix (ECM) hydrogel constructs for in vivo tissue mineralisation and formation. Stro-1-enriched human marrow stromal cells were incorporated together with select growth factors including VEGF, TGF-β3, BMP-2, PTHrP VitD3, to augment formation, mixed alginate structural support. Growth delivered through fast (non-osteogenic factors) slow (osteogenic release PLGA microparticles. Constructs 5 mm...
The development of more complex in vitro models for the assessment novel drugs and chemicals is needed because limited biological relevance animal to humans as well ethical considerations. Although some human-cell-based assays exist, they are usually 2D, consist single cell type, have cellular functional representation native tissue. In this study, we used biomimetic porous electrospun scaffolds develop an immunocompetent 3D model human respiratory tract comprised three key types present...
A cell‐laden 3D network is formed upon the addition of avidin to biotinylated polymer particles in presence a suspension cells (see Figure). Applications tissue engineering and drug delivery are demonstrated; shape‐retaining biomaterial within minutes injecting components into cavity. The molecular interaction mechanism self‐assembly differentiates this polymer/cell composite from other injectable systems.
The development of growth factor delivery strategies to circumvent the burst release phenomenon prevalent in most current systems has driven research towards encapsulating molecules resorbable polymer matrices. For these techniques be efficacious a clinical setting, several key points need addressed. This present study investigated encapsulation factor, BMP-2 within PLGA/PLGA-PEG-PLGA microparticles. Morphology, size distribution, efficiency and kinetics were we have demonstrated sustained...