A5041702972- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Neurogenesis and neuroplasticity mechanisms
- Neuroendocrine regulation and behavior
- Genetics and Neurodevelopmental Disorders
- Neural dynamics and brain function
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroinflammation and Neurodegeneration Mechanisms
- Sleep and Wakefulness Research
- Infant Health and Development
- Ion channel regulation and function
- Zebrafish Biomedical Research Applications
- Autism Spectrum Disorder Research
- Cancer-related molecular mechanisms research
- Epilepsy research and treatment
- Underwater Vehicles and Communication Systems
- Acoustic Wave Phenomena Research
- Photoreceptor and optogenetics research
- Genomics and Rare Diseases
- Hearing Loss and Rehabilitation
- Connexins and lens biology
- Neuroscience and Neural Engineering
- Memory and Neural Mechanisms
- Neonatal and fetal brain pathology
University of Washington
2003-2020
Seattle University
2018
University of California, San Francisco
2009-2014
Neurological Surgery
2009-2014
Broad Center
2012
Epilepsy, a disease characterized by abnormal brain activity, is disabling and potentially life-threatening condition for nearly 1% of the world population. Unfortunately, modulation excitability using available antiepileptic drugs can have serious side effects, especially in developing brain, some patients only be improved surgical removal regions containing seizure focus. Here, we show that bilateral transplantation precursor cells from embryonic medial ganglionic eminence (MGE) into early...
We report functional and structural evidence for GluA2-lacking Ca<sup>2+</sup>-permeable AMPARs (CP-AMPARs) at the mature hair cell ribbon synapse. By using methodological advantages of three species (of either sex), we demonstrate that CP-AMPARs are present synapse in an evolutionarily conserved manner. Via a combination <i>in vivo</i> electrophysiological Ca<sup>2+</sup> imaging approaches larval zebrafish, show stimulation leads to robust influx into afferent terminals. Prolonged...
Ethanol potentiates glycinergic synaptic transmission to hypoglossal motoneurons (HMs). This effect on changes with postnatal development in that juvenile HMs (P9-13) are more sensitive ethanol than neonate (P1-3). We have now extended our previous study investigate modulation of GABA(A) receptors (GABA(A)Rs), because both GABA and glycine mediate inhibitory brain stem motoneurons. tested the effects GABAergic miniature postsynaptic currents (mIPSCs) recorded from rat an vitro slice...
Mitochondria play a prominent role in mechanosensory hair cell damage and death. Although cells are thought to be energetically demanding cells, how mitochondria respond these demands this might relate death is largely unexplored. Using genetically encoded indicators, we found that mitochondrial calcium flux oxidation regulated by mechanotransduction demonstrate activity has both acute long-term consequences on function. We tested whether variation reflected differences the vulnerability of...
Heterozygous de novo variants in the eukaryotic elongation factor EEF1A2 have previously been described association with intellectual disability and epilepsy but never functionally validated. Here we report 14 new individuals heterozygous variants. We validate multiple as protein-damaging using heterologous expression complementation analysis. Our findings allow us to confirm pathogenic broaden phenotypic spectrum include dystonia/choreoathetosis, some cases a degenerative course cerebral...
Abstract Within the hippocampus and neocortex, GABA is considered to be excitatory in early development due a relatively depolarized Cl − reversal potential ( E ). Although depolarizing nature of synaptic GABAergic events has been well established, it unknown whether cortical tonic currents mediated by extrasynaptically located A receptors (GABA Rs) are also excitatory. Here we examined neocortex their effect on neuronal excitability. Mean current, recorded from layer 5 (L5) pyramidal cells...
Neurons within the intact respiratory network produce bursts of action potentials that cause inspiration or expiration. Within inspiratory bursts, activity is synchronized on a shorter timescale to generate clusters occur in set frequency range and are called synchronous oscillations. We investigated how GABA glycine modulate oscillations rhythm during postnatal development. recorded from hypoglossal nerves using vitro rhythmically active mouse medullary slice preparation P0-P11 mice....
LIS1 gene mutations lead to a rare neurological disorder, classical lissencephaly, characterized by brain malformations, mental retardation, seizures, and premature death. Mice heterozygous for Lis1 ( +/− ) exhibit cortical defects in neuronal migration, increased glutamate-mediated synaptic transmission, spontaneous electrographic seizures. Recent work demonstrated that utero treatment of mutant dams with ALLN, calpain inhibitor, partially rescues migration the offspring. Given challenges...
The effects of ketamine on properties mouse hypoglossal nerve inspiratory bursts (I-bursts) were studied in vivo and vitro. In spontaneously breathing urethane anesthetized mice we observed rhythmic I-phase activity only 1 8 P9 pups. contrast older (≥ P10) was almost always observed. Ketamine caused a reduction I-burst frequency an increase peak integrated all three age groups (P10–P13, P15–P20 adult mice). these synchronous oscillations control after ketamine. Synchronous occur when...