A5086490646- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Infection Control and Ventilation
- Gastric Cancer Management and Outcomes
- interferon and immune responses
- Esophageal Cancer Research and Treatment
- Immune Response and Inflammation
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Infection Control in Healthcare
- Esophageal and GI Pathology
- Effects and risks of endocrine disrupting chemicals
- Toxic Organic Pollutants Impact
- Viral Infections and Vectors
- Virus-based gene therapy research
- COVID-19 Clinical Research Studies
- COVID-19 and healthcare impacts
- Hepatitis Viruses Studies and Epidemiology
- Gastrointestinal Tumor Research and Treatment
- Virology and Viral Diseases
- Dental Research and COVID-19
- Liver Disease and Transplantation
- Viral Infections and Immunology Research
Rakuno Gakuen University
2003-2025
Kyoto Prefectural University of Medicine
2015-2024
Osaka University
2007-2012
Hong Kong Baptist University
2009
Southern Medical University
2009
University of Houston
2009
Abstract Background The stability of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on human skin remains unknown, considering hazards viral exposure to humans. We generated a model that allows safe reproduction clinical studies application pathogens and elucidated SARS-CoV-2 skin. Methods evaluated influenza A virus (IAV), mixed with culture medium or upper mucus, surfaces dermal disinfection effectiveness 80% (weight/weight) ethanol against IAV. Results IAV were...
The increased infectivity and transmissibility of SARS-CoV-2 variants concern (VOCs) could cause significant human economic damage. Hence, understanding their characteristics is crucial to control infection. We evaluated the environmental stability Wuhan strain all VOCs (Alpha, Beta, Gamma, Delta, Omicron BA.1, BA.2 variants) on plastic skin surfaces disinfection efficacy.To evaluate stability, residual virus titres were measured over time. Their survival time half-life calculated using...
Host cellular proteases induce influenza virus entry into cells by cleaving the viral surface envelope glycoprotein hemagglutinin (HA). However, details on involved in this event are not fully available. We report here that ubiquitous type II transmembrane serine proteases, MSPL and its splice variant TMPRSS13, novel candidates for processing HA proteins of highly pathogenic avian (HPAI) viruses, apart from previously identified furin proprotein convertases 5 6. HAs all HPAI H5 H7 strains...
Abstract SARS-CoV-2 has evolved continuously and accumulated spike mutations with each variant having a different binding for the cellular ACE2 receptor. It is not known whether interactions between such mutated spikes glycans are conserved among lineages. Here, we focused on three glycosylation sites (53, 90 322) that geometrically close to investigated effect of their pattern affinity. These deletions caused distinct site-specific changes in acted cooperatively. Of note, particular...
Abstract SARS-CoV-2 variants of concern (VOCs) could cause significant human and economic damage owing to increased infectivity transmissibility, understanding their characteristics is crucial for infection control. Here, we analyzed differences in viral stability disinfection efficacy between the Wuhan strain all VOCs. On plastic skin surfaces, Alpha, Beta, Delta, Omicron exhibited more than two-fold longer survival strain, variant had longest time. Specifically, times Alpha variant, Beta...
Introduction: Various therapeutic agents are being developed for the treatment of coronavirus disease 2019 (COVID-19). Therefore, it is crucial to accumulate information regarding features drug-resistant viruses these antiviral drugs. Methods: We investigated emergence dual-drug resistance in a kidney transplant recipient who received sotrovimab (from day 0) and remdesivir (RDV) 8 17). sequenced whole viral genomes from nasopharyngeal swabs taken on 0 seven points after starting (on days 12,...
In recent years, the highly pathogenic avian influenza virus H5N1 has raised serious worldwide concern about an pandemic; however, biology of pathogenesis is largely unknown. To elucidate mechanism pathogenesis, we prepared primary airway epithelial cells from alveolar tissues 1-year-old pigs and measured growth kinetics three H5 viruses (A/Crow/Kyoto/53/2004 [H5N1], A/Duck/Hong Kong/342/78 [H5N2], Kong/820/80 [H5N3]), resultant cytopathicity, possible associated mechanisms. H5N1, but not...
Both antiseptic hand rubbing (AHR) using ethanol-based disinfectants (EBDs) and washing (AHW) are important means of infection control to prevent seasonal influenza A virus (IAV) outbreaks. However, previous reports suggest a reduced efficacy ethanol disinfection against pathogens in mucus. We aimed elucidate the situations mechanisms underlying EBDs IAV infectious evaluated inactivation concentration change IAV-infected patients' mucus (sputum). Additionally, AHR AHW effectiveness adhering...
ABSTRACT In this study, we show that the highly pathogenic H5N1 avian influenza virus (AIV) (A/crow/Kyoto/53/04 and A/chicken/Egypt/CL6/07) induced apoptosis in duck embryonic fibroblasts (DEF). contrast, was reduced among cells infected with low-pathogenic AIVs (A/duck/HK/342/78 [H5N2], A/duck/HK/820/80 [H5N3], A/wigeon/Osaka/1/01 [H7N7], A/turkey/Wisconsin/1/66 [H9N2]). Thus, investigated molecular mechanisms of by H5N1-AIV infection. Caspase-dependent -independent pathways contributed to...
ABSTRACT A change in viral hemagglutinin (HA) receptor binding specificity from α2,3- to α2,6-linked sialic acid is necessary for highly pathogenic avian influenza (AI) virus subtype H5N1 become pandemic. However, details of the human-adaptive remain unknown. Our database search clade 2.2.1 viruses circulating Egypt identified multiple HA mutations that had been selected infected patients. Using reverse genetics, we found increases both human and pH threshold membrane fusion were facilitate...
A major determinant in the change of avian influenza virus host range to humans is E627K substitution PB2 polymerase protein. However, activity viruses with a single PB2-E627K mutation still lower than that seasonal human viruses, implying require mutations addition PB2-627K for adaptation. Here, we used database search H5N1 clade 2.2.1 sequences identify other adaptation have been selected infected patients. Several identified acted cooperatively increase viral growth airway epithelial...
Although viral RNA or infectious virions have been detected in the feces of individuals infected with human influenza A and B viruses (IAV/IBV), mechanism survival gastrointestinal tract remains unclear. We developed a model that attempts to recapitulate conditions encountered by swallowed virus. While IAV/IBV are vulnerable simulated digestive juices (gastric acid bile/pancreatic juice), highly viscous mucus protects virions, allowing virus retain its infectivity. Our results suggest...
ABSTRACT Influenza A virus (IAV) affects the upper and lower respiratory tracts rapidly induces expression of mucins, which are common O-glycosylated proteins, on epithelial surfaces tract. Although mucin production is associated with inhibition transmission as well characteristic clinical symptoms, little known regarding how mucins produced cells they affect IAV replication. In this study, we found that two microRNAs (miRNAs), miR-17-3p miR-221, target GalNAc transferase 3 (GALNT3) mRNA,...
Avian influenza virus H9N2 has been endemic in birds the Middle East, particular Egypt with multiple cases of human infections since 1998. Despite concerns about pandemic threat posed by H9N2, little is known biological properties this epicentre infection. Here, we investigated evolutionary dynamics East and identified phylogeny-associated PB2 mutations that acted cooperatively to increase replication/transcription cells. The accumulation also correlated an growth upper lower airways mice...
The development of a simple detection method with high sensitivity is essential for the diagnosis and surveillance infectious diseases. Previously, we constructed sensitive biosensor pathological human influenza viruses using boron-doped diamond electrode terminated sialyloligosaccharide receptor-mimic peptide that could bind to hemagglutinins involved in viral infection. Circulation induced by avian virus humans has become major public health concern, methods are urgently needed. Here,...
Flavonoids have poor bioavailabilities largely because of metabolism via UDP-glucuronosyltransferases (UGTs). This study aims to further understand the functions UGT in metabolizing genistein and apigenin, two compounds metabolized more extensively gut than liver. Because Gunn rats are deficient UGT1As, we determined whether this deficiency would result less flavonoid glucuronidation, using rat intestinal perfusion model microsomes prepared from liver intestine. In yeast-expressed isoforms,...
Highly pathogenic avian influenza virus H5N1 has spread across Eurasia and Africa, outbreaks are now endemic in several countries, including Indonesia, Vietnam Egypt. Continuous circulation of Egypt, from a single infected source, led to significant genetic diversification with phylogenetically separable sublineages, providing an opportunity study the impact evolution on viral phenotypic variation. In this study, we analysed phylogeny H5 haemagglutinin (HA) genes viruses isolated Egypt 2006...
Close interaction between avian influenza (AI) viruses and humans in Egypt appears to have resulted many of the worldwide cases human infections by both H5N1 H9N2 AI viruses. is regarded as a hot spot virus evolution. Although no natural reassortant has been reported so far, their cocirculation may allow emergence reassortants that present significant public health risk. Using reverse genetics, we report here first comprehensive data showing H5N1-N9N2 fairly high genetic compatibility...
Highly pathogenic avian influenza virus H5N1 infects a wide range of host species, with few cases sporadic pigeon infections reported in the Middle East and Asia. However, role pigeons ecology evolution viruses remains unclear. We previously two strains, isolated from naturally infected Egypt, that have several unique mutations their viral polymerase genes. Here, we investigated effect these on activity growth identified three affected activity. The results showed PB1-V3D mutation...