A5015462363- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Plant responses to water stress
- Plant-Microbe Interactions and Immunity
- Postharvest Quality and Shelf Life Management
- Plant nutrient uptake and metabolism
- Phytochemical Studies and Bioactivities
- Light effects on plants
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Lepidoptera: Biology and Taxonomy
- Coastal wetland ecosystem dynamics
- Plant tissue culture and regeneration
- Rice Cultivation and Yield Improvement
Chung-Ang University
2016-2023
Grain size and leaf angle are two important traits determining grain yield in rice. However, the mechanisms regulating remain largely unknown. Here, we characterized a rice gain-of-function mutant, slender Dominant (slg-D), which exhibited longer narrower grains larger angles, similar to plants with elevated brassinosteroid (BR) levels or strengthened BR signaling. The increased cell length is responsible for mutant phenotypes slg-D. We demonstrated that phenotype of slg-D caused by enhanced...
DWARF1 (DWF1) is a sterol C-24 reductase that catalyses the conversion of 24-methylenecholesterol (24-MCHR) to campesterol (CR) in Arabidopsis. A loss-of-function mutant, dwf1, showed similar phenotypic abnormalities brassinosteroid (BR)-deficient mutants. These were reversed wild-type phenotype by exogenous application castasterone (CS) and brassinolide (BL), but not dolichosterone (DS). Accumulation DS decreased CS found quantitative analysis endogenous BRs dwf1. The enzyme solution...
Ethylene regulates numerous aspects of plant growth and development. Multiple external internal factors coordinate ethylene production in tissues. Transcriptional post-translational regulations ACC synthases (ACSs), which are key enzymes mediating a rate-limiting step biosynthesis have been well characterized. However, the regulation physiological roles oxidases (ACOs) that catalyze final largely unknown Arabidopsis. Here, we show Arabidopsis
ProACO4–GUS expression and RT-PCR analysis revealed that ACO4 is predominantly expressed in shoots of Arabidopsis seedlings under light conditions. ACO4-overexpressed mutant 35S–ACO4 produced more ethylene relative to the wild-type, which resulted reduced growth seedlings. The abnormal recurred after application Co2+ ions, suggesting a functional ACO necessary regulate development Exogenously-applied brassinosteroids (BRs) inhibited ACO4, an enhanced was found det2, BR-deficient mutant....
Gas chromatography–mass spectrometry (GC–MS) analysis revealed that castasterone and its biosynthetic precursors are found in Brachypodium distachyon. In vitro conversion experiments with crude enzyme solutions prepared from B. distachyon demonstrated the presence of following sequences: campesterol → campesta-4-en-3-one campesta-3-one campestanol 6-deoxocathasterone 6-deoxoteasterone teasterone ↔ 3-dehydroteasterone typhasterol castasterone. 22-hydroxycampesterol...
Brassinosteroids (BRs) are naturally occurring steroidal hormones that play diverse roles in various processes during plant growth and development. Thus, genetic manipulation of endogenous BR levels might offer a way improving the agronomic traits crops, including architecture stress tolerance. In this study, we produced transgenic creeping bentgrass (Agrostis stolonifera L.) overexpressing BR-inactivating enzyme, Arabidopsis thaliana BR-related acyltransferase 1 (AtBAT1), which is known to...
Brassinosteroids (BRs) are known to be endogenous regulators of ethylene production, suggesting that some BR activity in plant growth and development is associated with ethylene. Here, we demonstrated production Arabidopsis thaliana roots increased by signaling via the biosynthetic gene for ACC oxidase 1 (ACO1). Electrophoretic mobility shift chromatin immune-precipitation assays showed transcription factor BES1 directly binds two E-box sequences located intergenic region ACO1. GUS...
Hipparchia autonoe (Esper, 1783) is a protected butterfly species found in Mt. Halla South Korea. We have determined mitochondrial genome of H. collected Halla. The circular mitogenome 15,300 bp long, which shorter than previously sequenced by 189 due to differences tandem repeats. It includes 13 protein-coding genes, 2 ribosomal RNA and 22 transfer RNAs. base composition was AT-biased (78.9%). Nineteen single nucleotide polymorphisms one insertion deletion were identified between the two...
Brachypodium distachyon is a monocotyledonous model plant that contains castasterone (CS) but no brassinolide (BL) as the end product of brassinosteroids (BR) biosynthesis, indicating dysfunction BL synthase, which catalyzes conversion CS to BL. To increase BR activity, Arabidopsis cytochrome P450 85A2 ( AtCYP85A2 ) encoding 6-oxidase/BL synthase was introduced into B. with seed-specific promoters pBSU1, pAt5g10120 , and pAt5g54000 . RT-PCR analysis GUS activity revealed only expressed in...
Abstract Unlike the indispensable function of steroid hormone brassinosteroid (BR) in regulating plant growth and development, metabolism secondary metabolites regulated by BR is not well known. Here we show that reduces carotenoid accumulation Arabidopsis seedlings. BR-deficient or BR-insensitive mutants accumulated higher content carotenoids than wild-type plants, whereas treatment reduced content. We demonstrated transcriptionally suppresses 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD)...