A5053970026- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Phytochemical Studies and Bioactivities
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Phytoestrogen effects and research
- Postharvest Quality and Shelf Life Management
- Steroid Chemistry and Biochemistry
- Plant responses to water stress
- Traditional Chinese Medicine Studies
- Natural product bioactivities and synthesis
- Genomics and Phylogenetic Studies
- Microbial bioremediation and biosurfactants
- Light effects on plants
- Ecology and Conservation Studies
- Cholesterol and Lipid Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Bryophyte Studies and Records
- Healthcare and Venom Research
Chung-Ang University
2015-2024
Chung Cheong University
2013
University of Toronto
2010
University of California, San Diego
2010
RIKEN Center for Sustainable Resource Science
2010
Ithaca College
2010
Cornell University
2010
Daewoo Shipbuilding and Marine Engineering (South Korea)
2009
Inha Technical College
2009
Teikyo University
2009
Multiple transcription factors (TFs) play essential roles in plants under abiotic stress, but how these multiple TFs cooperate stress responses remains largely unknown. In this study, we provide evidence that the NAC (for NAM, ATAF1/2, and CUC2) TF ANAC096 cooperates with bZIP-type ABRE binding factor protein (ABF/AREB) to help survive dehydration osmotic conditions. directly interacts ABF2 ABF4, not ABF3, both vitro vivo. synergistically activate RD29A transcription. Our genome-wide gene...
Abstract The trade-off between growth and immunity is crucial for survival in plants. However, the mechanism underlying growth-immunity balance has remained elusive. PRE-IBH1-HBI1 tripartite helix-loop-helix/basic helix-loop-helix module part of a central transcription network that mediates regulation by several hormonal environmental signals. Here, genome-wide analyses HBI1 target genes show regulates both overlapping unique targets compared with other DNA binding components Arabidopsis...
The conversion of castasterone (CS) to brassinolide (BL), a Baeyer-Villiger oxidation, represents the final and rate-limiting step in biosynthesis BL plants. Heterologously expressed Arabidopsis thaliana CYP85A2 yeast mediated CS as well C-6 oxidation brassinosteroids (BRs). This indicated that is bifunctional enzyme possesses BR oxidase synthase activity. thus cytochrome P450 mediates Biochemical, physiological, molecular genetic analyses loss-of-function overexpression lines demonstrated...
Gibberellins (GAs) and brassinosteroids (BRs), two growth-promoting phytohormones, regulate many common physiological processes. Their interactions at the molecular level remain unclear. Here, we demonstrate that OsGSR1, a member of GAST (GA-stimulated transcript) gene family, is induced by GA repressed BR. RNA interference (RNAi) transgenic rice plants with reduced OsGSR1 expression show phenotypes similar to deficient in BR, including short primary roots, erect leaves fertility. The RNAi...
MicroRNA (miR)390 cleaves the non-coding TAS3 precursor RNA for production of tasiRNA-ARF, a group an endogenous trans-acting small-interfering RNAs which cleave transcripts auxin response factor (ARF) 3/4. miR390-cleaved is polymerized and diced into tasiRNA-ARF by RNA-dependent polymerase6 (RDR6) Dicer-like4 (DCL4), respectively. tasiRNA-ARF-dependent post-transcriptional gene silencing (PTGS) ARF3/4 involved in auxin-mediated polarity establishment development aerial lateral organs, such...
Rice architecture is an important agronomic trait and a major limiting factor for its high productivity. Here we describe novel CCCH-type zinc finger gene, OsLIC (Oraza sativaleaf tiller angle increased controller), which involved in the regulation of rice plant architecture. encoded ancestral unique CCCH type finge protein. It has many orthologous other organisms, ranging from yeast to humane. Suppression endogenous expression resulted drastically leaf angles, shortened shoot height,...
Arabidopsis RAV1, RAV1L and RAV2/TEM2 are Related to ABI3/VP1 (RAV) transcription factors that contain both plant-specific B3 AP2 domains. RAV1 was known be a negative regulator of growth its transcript level repressed by brassinolide (BL). In this study, we found the expressions closest homologs RAV2 were also regulated other plant hormones, especially significantly BL abscisic acid (ABA), which mediate various abiotic stress responses in plants. Therefore, further investigate physiological...
Abstract Plants need to finely balance resources allocated growth and immunity achieve optimal fitness. A tradeoff between pathogen-associated molecular pattern (PAMP)-triggered (PTI) brassinosteroid (BR)-mediated was recently reported, but more information about the underlying mechanisms is needed. Here, we identify basic helix-loop-helix (bHLH) transcription factor HOMOLOG OF BRASSINOSTEROID ENHANCED EXPRESSION2 INTERACTING WITH IBH1 (HBI1) as a negative regulator of PTI signaling in...
Grain size and leaf angle are two important traits determining grain yield in rice. However, the mechanisms regulating remain largely unknown. Here, we characterized a rice gain-of-function mutant, slender Dominant (slg-D), which exhibited longer narrower grains larger angles, similar to plants with elevated brassinosteroid (BR) levels or strengthened BR signaling. The increased cell length is responsible for mutant phenotypes slg-D. We demonstrated that phenotype of slg-D caused by enhanced...
Summary Phytosterols are membrane components or precursors for brassinosteroid ( BR ) biosynthesis. As they cannot be transported long distances, their homeostasis is tightly controlled through biosynthesis and metabolism. However, it unknown whether micro RNA s involved in homeostatic regulation. Rice Oryza sativa plants transformed with microRNA osa‐miR1848 its target, the obtusifoliol 14α‐demethylase gene, Os CYP 51G3 , were used to investigate role of regulation phytosterol directs mRNA...
ABSTRACT Rhodococcus sp. strain DK17 was isolated from soil and analyzed for the ability to grow on o- xylene as sole carbon energy source. Although cannot m - p -xylene, it is capable of growth benzene, phenol, toluene, ethylbenzene, isopropylbenzene, other alkylbenzene isomers. One UV-generated mutant strain, DK176, simultaneously lost o although could still phenol. The also unable oxidize indole indigo following in presence -xylene. This observation suggests loss an oxygenase that...
Abstract Exogenously applied brassinolide (BL, 10−9–10−5 m) increased gravitropic curvature in maize (Zea mays) primary roots. The BL-enhanced was clearly promoted the presence of indole-3-acetic acid (IAA, 10−10–10−8 m), indicating that BL is interactive with IAA during response. effect between and completely diminished by treatment p-chlorophenoxy isobutric acid, an auxin action antagonist. activation response absence nullified application 2,3,5-triiodobenzoic a polar transport inhibitor....
A number of Arabidopsis (Arabidopsis thaliana) lesion-mimic mutants exhibit alterations in both abiotic stress responses and pathogen resistance. One these mutants, constitutive expresser PR genes22 (cpr22), which has a mutation two cyclic nucleotide-gated ion channels, is typical mutant exhibiting elevated levels salicylic acid (SA), spontaneous cell death, expression defense-related genes, enhanced resistance to various pathogens; the majority its phenotypes are SA dependent. These defense...
Summary Organ size is determined by cell number and size, involves two fundamental processes: proliferation expansion. Although several plant hormones are known to play critical roles in shaping organ regulating the cycle, it not whether brassinosteroids (BRs) also involved division. Here we identified a rice T‐DNA insertion mutant for referred as xiao , that displays dwarfism erect leaves, typical BR‐related phenotypes, together with reduced seed setting. XIAO predicted encode an LRR...
Summary Brassinosteroids ( BR s) are essential regulators of plant architecture. Understanding how s control height and leaf angle would facilitate development new type varieties by biotechnology. A number mutants involved in biosynthesis have been isolated but many them lack detailed genetic analysis. Here, we report the isolation characterization a severe dwarf mutant, chromosome segment deleted 1 csdd1 ), which was deficient rice. We mutant screening tissue culture‐derived population,...
Brassinosteroid (BR) regulates a wide range of physiological responses through the activation BRASSINAZOLE RESISTANT1 (BZR1), whose activity is tightly controlled by its phosphorylation status and degradation. Although BZR1 appears to be degraded in distinct ways response different hormonal or environmental cues, little known about how BR signaling Here we show that BR-regulated U-box protein PUB40 mediates proteasomal degradation root-specific manner Arabidopsis (Arabidopsis thaliana)....
DWARF1 (DWF1) is a sterol C-24 reductase that catalyses the conversion of 24-methylenecholesterol (24-MCHR) to campesterol (CR) in Arabidopsis. A loss-of-function mutant, dwf1, showed similar phenotypic abnormalities brassinosteroid (BR)-deficient mutants. These were reversed wild-type phenotype by exogenous application castasterone (CS) and brassinolide (BL), but not dolichosterone (DS). Accumulation DS decreased CS found quantitative analysis endogenous BRs dwf1. The enzyme solution...
The effects of ABA and putrescine, a polyamine, on cold-induced membrane leakage were investigated using primary leaves wild-type an ABA-deficient mutant, flacca, tomato (Lycopersicon esculentum Mill.). amount chilling-induced electrolyte from flacca was much higher than that the leaves. When applied exogenously reduced both mutant. However, not as pronounced in indicating is important mediator response to cold stress Putrescine Synthesis putrescine increased by treatment. DFMO, biosynthetic...
The plasma membrane-localized BRASSINOSTEROID-INSENSITIVE1 (BRI1) and BRI1-ASSOCIATED KINASE1 (BAK1) are a well-known receptor pair involved in brassinosteroids (BR) signaling Arabidposis. formation of complex response to BRs the subsequent activation cytoplasmic domain kinase activity share mechanistic characteristics with animal kinases. Here, we demonstrate that BRI1 BAK1 BR-dependently phosphorylated, phosphorylated forms two proteins persist for different lengths time. Mutations either...
Summary Brassinosteroids ( BR s) are essential for various aspects of plant development. Cellular homeostasis is critical proper growth and development plants; however, its regulatory mechanism remains largely unknown. BAT1 ‐related acyltransferase 1 ), a gene encoding putative acyltransferase, was found to be involved in vascular bundle full‐length cDNA over‐expressor FOX ) screen. Over‐expression BAT resulted typical ‐deficient phenotypes, which were rescued by exogenously applied...