A5077150143- Venomous Animal Envenomation and Studies
- Amphibian and Reptile Biology
- Rabies epidemiology and control
- Animal Behavior and Reproduction
- Biochemical and Structural Characterization
- Marine Invertebrate Physiology and Ecology
- Insect and Pesticide Research
- Advanced Proteomics Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Healthcare and Venom Research
- RNA modifications and cancer
- Ion channel regulation and function
- Vector-borne infectious diseases
- RNA Research and Splicing
- Yersinia bacterium, plague, ectoparasites research
- Peroxisome Proliferator-Activated Receptors
- Cell Adhesion Molecules Research
- Cardiac Fibrosis and Remodeling
- Tissue Engineering and Regenerative Medicine
- Neurobiology and Insect Physiology Research
- Immune Cell Function and Interaction
- Protease and Inhibitor Mechanisms
- Mass Spectrometry Techniques and Applications
- Cancer, Lipids, and Metabolism
- Signaling Pathways in Disease
University of Colorado Anschutz Medical Campus
2021-2025
Scripps Research Institute
2018-2025
University of Colorado Denver
2022-2025
University of Northern Colorado
2012-2024
University of Colorado Cancer Center
2024
Liverpool School of Tropical Medicine
2024
University of California, Riverside
2024
Denver Health Medical Center
2024
Health and Hospital Corporation
2024
Consejo Superior de Investigaciones Científicas
2017-2024
In pancreatic ductal adenocarcinoma (PDAC) patients, we show that response to radiation therapy (RT) is characterized by increased IL-2Rβ and IL-2Rγ along with decreased IL-2Rα expression. The bispecific PD1-IL2v a PD-1-targeted IL-2 variant (IL-2v) immunocytokine engineered cis targeted PD-1 abolished binding, which enhances tumor-antigen-specific T cell activation while reducing regulatory (Treg) suppression. Using in orthotopic PDAC KPC-driven tumor models, marked improvement local...
Data-independent acquisition (DIA) is a promising technique for the proteomic analysis of complex protein samples. A number studies have claimed that DIA experiments are more reproducible than data-dependent (DDA), but these claims unsubstantiated since different data methods used in two methods. Data most workflows depends on spectral library searches, whereas DDA typically employs sequence database searches. In this study, we examined reproducibility and results using both search. The...
The extracellular matrix (ECM) is a critical non-cellular component of multicellular organisms containing variety proteins, glycoproteins, and proteoglycans which have been implicated in wide essential biological processes, including development, wound healing, aging. Due to low solubility, many ECM proteins underrepresented previous proteomic datasets. Using an optimized three-step decellularization extraction method involving chaotrope digestion via hydroxylamine hydrochloride, we...
Abstract Background Snake venoms are trophic adaptations that represent an ideal model to examine the evolutionary factors shape polymorphic traits under strong natural selection. Venom compositional variation is substantial within and among venomous snake species. However, forces shaping this phenotypic complexity, as well potential integrated roles of biotic abiotic factors, have received little attention. Here, we investigate geographic in venom composition a wide-ranging rattlesnake (...
Snakebite envenoming is a neglected tropical disease that causes substantial mortality and morbidity globally. The venom of African spitting cobras often permanent injury via tissue-destructive dermonecrosis at the bite site, which ineffectively treated by current antivenoms. To address this therapeutic gap, we identified etiological toxins in Naja nigricollis responsible for causing local dermonecrosis. While cytotoxic three-finger were primarily cobra cytotoxicity cultured keratinocytes,...
Abstract The restrictor, ZC3H4/WDR82, is the major termination factor for antisense transcription from bidirectional promoters, but its mechanism poorly understood. We report that ZC3H4/WDR82 co-purifies with PP1 phosphatase and nuclear targeting subunit, PNUTS, which binds directly to WDR82 subunit of restrictor. AlphaFold predicts a quaternary complex, PPWZ, in P P1-associated NUTS Z C3H4 both contact W DR82. To investigate role protein dephosphorylation PPWZ activity, we expressed...
Animal venoms represent a diverse source of potentially valuable therapeutic compounds due to the high specificity and potent biological activity many toxins. Snake venom toxins, particularly disintegrins proteases from viper venoms, have yielded therapeutics with anti-cancer hemostatic dysfunction activities. However, rear-fanged "colubrid" snakes rarely been analyzed perspective potential lead compound development. Here, we discuss recent progress in analysis these focusing on several...
Snake venoms represent an adaptive trophic response to the challenges confronting a limbless predator for overcoming combative prey, and this chemical means of subduing prey shows several dominant phenotypes. Many front-fanged snakes, particularly vipers, feed on various vertebrate invertebrate species, some their venom components (e.g., metalloproteinases, cobratoxin) appear have been selected "broad-brush" incapacitation different taxa. Using proteomic genomic techniques, compositional...
Snakebite envenoming is a neglected tropical disease that may claim over 100,000 human lives annually worldwide. occurs as the result of an interaction between and snake elicits either defensive response from or, more rarely, feeding mistaken identity. therefore biological and, specifically, ecological problem. Snake venom itself often described "cocktail", it heterogenous mixture molecules including toxins (which are typically proteinaceous) responsible for pathophysiological consequences...
Pelvic floor disorders, including pelvic organ prolapse and urinary fecal incontinence, affect millions of women globally represent a major public health concern. muscle (PFM) dysfunction has been identified as one the leading risk factors for development these morbid conditions. Childbirth, specifically vaginal delivery, recognized most important potentially modifiable factor PFM injury; however, precise mechanisms after parturition remain elusive. In this study, we demonstrated that PFMs...
Abstract Background Vertebrate predators use a broad arsenal of behaviors and weaponry for overcoming fractious potentially dangerous prey. A unique array predatory strategies occur among snakes, ranging from mechanical modes constriction jaw-holding in non-venomous to chemical means, venom, quickly dispatching However, even venomous different prey handling are utilized, varying the strike-and-hold exhibited by highly toxic elapid snakes rapid strike-and-release envenomation seen viperid...
Seminal plasma is a critical and complex fluid that carries sperm to eggs initiate the fertilization process. Here, we present top-down mass spectrometry (TDMS) strategy for identifying proteins posttranslational modifications (PTMs) in bovine seminal plasma. In this study, were separated using sheathless capillary zone electrophoresis (CZE)-MS reversed-phase liquid chromatography (LC)-MS, then fragmented electron-transfer/higher-energy collisional dissociation (EThcD) 213 nm ultraviolet...
The implementation of cancer immunotherapies has seen limited clinical success in head and neck squamous cell carcinoma (HNSCC). Interleukin-2 (IL-2), which modulates the survival functionality lymphocytes, is an attractive target for new but one that by presence regulatory T cells (Tregs) expressing high-affinity IL-2Rα. bispecific immunocytokine PD1-IL2v preferentially delivers IL-2 signaling through IL-2Rβγ on PD-1-expressing cells. Selectively targeting intermediate-affinity can be...
The adult cardiac extracellular matrix (ECM) is largely comprised of type I collagen. In addition to serving as the primary structural support component ECM, collagen also provides an organizational platform for other ECM proteins, matricellular and signaling components that impact cellular stress sensing in vivo.
Abstract Aims Following myocardial infarction (MI), the heart repairs itself via a fibrotic repair response. The degree of fibrosis is determined by balance between deposition extracellular matrix (ECM) activated fibroblasts and breakdown nascent scar tissue proteases that are secreted predominantly inflammatory cells. Excessive proteolytic activity turnover has been observed in human failure, protease inhibitors injured regulate breakdown. Serine (Serpins) represent largest most...
As trophic adaptations, rattlesnake venoms can vary in composition depending on several intrinsic and extrinsic factors. Ontogenetic changes venom have been documented for numerous species, but little is known of the potential age-related many species found México. In current study, samples collected from adult neonate Crotalus polystictus Estado de México were subjected to enzymatic electrophoretic analyses, toxicity assays (LD50), MALDI-TOF mass spectrometry, a pooled sample was analyzed...