A5089200400- Histone Deacetylase Inhibitors Research
- Glioma Diagnosis and Treatment
- Signaling Pathways in Disease
- Meningioma and schwannoma management
- Pituitary Gland Disorders and Treatments
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- PARP inhibition in cancer therapy
- Cancer Immunotherapy and Biomarkers
- Neural dynamics and brain function
- Nuclear Receptors and Signaling
- Ferroptosis and cancer prognosis
- Cancer Research and Treatments
- Head and Neck Surgical Oncology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Peptidase Inhibition and Analysis
- Atherosclerosis and Cardiovascular Diseases
- S100 Proteins and Annexins
- Complement system in diseases
First Hospital of China Medical University
2015-2025
China Medical University
2015-2025
Nanjing Medical University
2013-2025
Academy of Medical Sciences
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Peking University
2024
First Hospital of Jiaxing
2017-2023
Jiaxing University
2020-2023
Jiangsu Province Hospital
2021
Case Western Reserve University
2018
Abstract Purpose: Glioma tissues consist of not only glioma cells but also glioma-associated nontumor cells, such as stromal and immune cells. These dilute the purity play important roles in biology. Currently, implications variation are sufficiently clarified. Experimental Design: Here, tumor was inferred for 2,249 gliomas 29 normal brain from 5 cohorts. Based on transcriptomic profiling method, we classified CGGA TCGA-RNAseq cohorts RNAseq set discovery. Cases TCGA-microarray, REMBRANDT,...
T lymphocyte infiltration has been detected in glioma, although its significance remains unclear. The purpose of the present study was to explore prognostic value CD4+ and CD8+ tumour-infiltrating lymphocytes (TILs) infiltrating Forkhead box protein 3 (FoxP3+) regulatory cells were also investigated. CD4+, FoxP3+ TILs assessed by immunohistochemical staining tissue microarray cores from 284 gliomas. Kaplan–Meier analysis Cox proportional hazards models used examine survival function these 90...
Markers of systemic inflammation are correlated with patient survival in various cancers. The prognostic value neutrophil-to-lymphocyte ratio (NLR) was compared that platelet-to-lymphocyte (PLR) patients glioblastoma. association NLR neutrophil and T- cell infiltration also explored. A total 152 glioblastoma were retrospectively analyzed. Clinical information obtained from electronic medical records. Kaplan-Meier analysis the Cox proportional hazards models used to examine function...
To investigate the local immune status and its prognostic value in glioma.A cohort of 297 glioma samples with whole genome microarray expression data from Chinese Glioma Genome Atlas database were included for discovery. The Cancer (TCGA) was used validation. Principal components analysis gene set enrichment to explore bioinformatic implication.Distinct identified according histologic grade. Glioblastoma (GBM) exhibited an enhanced phenotype compared lower grade glioma. We profiled...
Insulin-like growth factor binding protein-2 (IGFBP-2) is significantly increased in the serum of patients with malignant gliomas. High plasma IGFBP-2 levels are correlated poor prognosis glioma patients. However, exact role exogenous gliomas unclear.Using MTT cell viability assay, cycle analysis, and transwell migration it was demonstrated that treatment stimulated proliferation invasion U87 U251 lines primary SU3 cells. Western blot analysis immunofluorescence staining revealed promoted...
Abstract Extensive infiltration by tumor-associated macrophages (TAM) in combination with myeloid-derived suppressor cells constitute the immunosuppressive microenvironment and promote malignant phenotype of gliomas. The aggressive mesenchymal (MES)-subtype glioma stem (GSC) are prominent However, underlying immune-suppressive mechanisms still unknown. current study showed that antitumor immune was activated Nfat1−/− mice, suggesting induction nuclear factor T cells-1 (NFAT1). In TAMs, NFAT1...
Serum albumin level is a reliable and convenient marker of the nutritional status patients, has been identified as prognostic in glioblastoma. However, because recent wide application standard radio-chemotherapy for treatment glioblastoma effect preoperative serum levels needs to be re-evaluated related mechanism should further explored.A total 214 patients with histologically proven who underwent at our institution between 2009 2012 were retrospectively analyzed. Clinical information was...
We previously demonstrated that the local immune status correlated with glioma prognosis. Interleukin-6 (IL6) was identified as an important immune-related risk marker related to unfavourable In this study, we further investigated role and regulation of IL6 signalling in glioma.The expression prognostic value receptor (IL6R) were explored The Cancer Genome Atlas (TCGA) REMBRANDT databases clinical samples. Functional effects genetic knockdown overexpression IL6R or stimulation examined vitro...
Members of the nuclear factor activated T cells (NFAT) family have been identified as regulators oncogenic transformation in several human malignancies. A prominent member this family, NFAT1, is associated with tumor cell survival, apoptosis, migration and invasion. Here, we investigated role NFAT1 glioma cells. In 111 clinical samples, microarray analysis demonstrated that was over-expressed glioblastoma multiforme (GBM), compared low-grade gliomas, a result confirmed by RT-PCR 24 samples...
Patients with ER/HER2-positive breast cancer have a poor prognosis and are less responsive to selective estrogen receptor modulators; this is presumably due the crosstalk between ER HER2. Fatty acid synthase (FASN) essential for survival maintenance of malignant phenotype cells. An intimate relationship exists FASN, We hypothesized that FASN may be downstream effector underlying ER/HER2 through PI3K/AKT/mTOR pathway in cancer. The present study implicated regulation expression cells...
// Wen Cheng 1 , Xiufang Ren 2 Jinquan Cai 3 Chuanbao Zhang 4,5 Mingyang Li Kuanyu Wang 6 Yang Liu Sheng Han and Anhua Wu Department of Neurosurgery, The First Hospital China Medical University, Shenyang, Pathology, Shengjing Second Affiliated Harbin Harbin, 4 Beijing Neurosurgical Institute, Beijing, 5 Tiantan Hospital, Capital Dalian Dalian, Correspondence to: Wu, email: Keywords : glioblastoma, MGMT promoter methylation, miRNA, prognosis, predictive model Received May 02, 2015 Accepted...
This study examined the ability of lipopolysaccharide (LPS) to affect glioma and stem-like cells (GSCs) in vitro induce antitumor immunity vivo role TLR4 these processes. Using RT-PCR immunohistochemistry, we expression 34 glioblastoma clinical samples. real time-PCR, western blot ELISA analyses, effect LPS stimulation on immune related molecules was evaluated RG2 U87 GSCs. Control or LPS-pretreated GSCs were intracranially subcutaneously implanted into wild-type nude Fisher 344 rats....
Abstract Glioma stem-like cells (GSC) promote tumor generation and progression. However, the mechanism of GSC induction or maintenance is largely unknown. We previously demonstrated that calcium-responsive transcription factor nuclear activated T cells-1 (NFAT1) in glioblastomas regulates invasion cells. In this study, we further explored role NFAT1 GSC. found expression was associated with an aggressive phenotype predicted poor survival gliomas. Compared normal glioma cells, upregulated...
Abstract Background Deregulation of the nuclear factor activated T cell (NFAT) pathway has been reported in several human cancers. Particularly, NFAT2 is involved malignant transformation tumor cells and identified as an oncogene. However, role glioblastoma (GBM) largely unknown. Methods The expression prognostic value were examined databases Repository Molecular Brain Neoplasia Data Cancer Genome Atlas (TCGA) clinical samples. functional effects silencing or overexpression evaluated glioma...
Special AT-rich sequence-binding protein 1 (SATB1) is a global chromatin organizer and gene regulator, high expression of SATB1 associated with progression poor prognosis in several malignancies. Here, we examine the pattern glioma. Microarray analysis 127 clinical samples showed that mRNA was expressed at lower levels highly malignant glioblastoma multiforme (GBM) than low-grade glioma normal brain tissue. This result further confirmed by real-time RT-PCR samples, three GBM cell lines,...
Objective This study explored the effects of telomerase reverse transcriptase (TERT) promoter mutations on transcriptional activity TERT gene under hypoxic and temozolomide (TMZ) treatment conditions, investigated status prognostic value these in gliomas. Methods The effect TMZ conditions was glioma cells using luciferase assay. were detected 101 samples (grades I–IV) 49 other brain tumors by sequencing. mRNA expression gliomas examined real-time PCR. Hazard ratios from survival analysis...
Abstract Curcumin has a variety of anticancer properties, but low bioavailability prevents its use in chemotherapeutic applications. To address this problem, we tested the efficacy synthetic curcumin analog B14 breast cancer cells and explored mechanism by which inhibits proliferation metastasis cells. We used cell line MCF‐7, MDA‐MB‐231 to study effects assessed viability, migration invasion, cycle, apoptosis, addition, antitumor effect vivo was examined mice bearing found that, as...
To explore the proteins regulated by cyclooxygenase-2 (COX-2) in gastric cancer, expression plasmid of COX-2siRNA was constructed and transfected into cancer cell line SGC7901. Then, two-dimensional electrophoresis PDQuest software analysis were applied to discover differentially expressed proteins. The differential protein spots analyzed matrix-assisted laser desorption/ionization time flight mass spectrometry. Fourteen between two lines identified. 15-Hydroxyprostaglandin dehydrogenase...
<title>Abstract</title> Hepatic ischemia-reperfusion injury (IRI) remains an unavoidable consequence of partial hepatic resection and liver transplantation. Oxidative stress damages cellular lipid membranes, causing the formation oxidized phospholipids (OxPLs), which are members damage-associated molecular patterns (DAMPs). Nevertheless, precise mechanism significance OxPLs in IRI yet to be investigated. Our findings reveal that accumulate excessively after IR. Compared control group,...