A5045316659- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Mitochondrial Function and Pathology
- Nutrition, Genetics, and Disease
- Neurobiology and Insect Physiology Research
- Adipokines, Inflammation, and Metabolic Diseases
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Advanced Proteomics Techniques and Applications
- Diet, Metabolism, and Disease
- Lipid metabolism and biosynthesis
- Ovarian function and disorders
- Sphingolipid Metabolism and Signaling
- 3D Printing in Biomedical Research
- ATP Synthase and ATPases Research
- Metabolomics and Mass Spectrometry Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Insect Utilization and Effects
- Reproductive Biology and Fertility
- Bioactive Compounds and Antitumor Agents
- Cancer Immunotherapy and Biomarkers
- Regulation of Appetite and Obesity
- Ion channel regulation and function
- Ion Channels and Receptors
The University of Sydney
2017-2024
Yale University
2023-2024
University of Virginia
2017-2024
Université Laval
2023
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Abstract The metabolic pathway of de novo lipogenesis is frequently upregulated in human liver tumours, and its upregulation associated with poor prognosis. Blocking cultured cancer cells sufficient to decrease cell viability; however, it not known whether blocking vivo can prevent tumorigenesis. Herein, we inhibit hepatic mice by liver-specific knockout acetyl-CoA carboxylase (ACC) genes treat the hepatocellular carcinogen diethylnitrosamine (DEN). Unexpectedly, lacking have a twofold...
Insulin triggers an extensive signaling cascade to coordinate adipocyte glucose metabolism. It is considered that the major role of insulin provide anabolic substrates by activating GLUT4-dependent uptake. However, stimulates phosphorylation many metabolic proteins. To examine implications this on metabolism, we performed dynamic tracer metabolomics in cultured adipocytes treated with insulin. Temporal analysis metabolite concentrations and labeling revealed rapid distinct changes favoring...
Adipose tissue is essential for metabolic homeostasis, balancing lipid storage and mobilization based on nutritional status. This coordinated by insulin, which triggers kinase signaling cascades to modulate numerous proteins, leading increased glucose uptake anabolic processes like lipogenesis. Given recent evidence that dispensable adipocyte respiration, we sought test whether necessary insulin-stimulated anabolism. Examining lipogenesis in cultured adipocytes, was insulin stimulate the...
Metabolic disease is caused by a combination of genetic and environmental factors, yet few studies have examined how these factors influence signal transduction, key mediator metabolism. Using mass spectrometry-based phosphoproteomics, we quantified 23,126 phosphosites in skeletal muscle five genetically distinct mouse strains two dietary environments, with without acute vivo insulin stimulation. Almost half the insulin-regulated phosphoproteome was modified background on an ordinary diet,...
•Adipocyte proteomes were highly conserved between white depots in lean mice.•Sustained obesogenic environment caused mitochondrial stress visceral adipocytes.•Subcutaneous adipose tissue adaptations could not be detected at the adipocyte level.•3T3-L1 total proteome was a good representation of adipocytes from mice. White is deposited mainly as subcutaneous (SAT), often associated with metabolic protection, and abdominal/visceral tissue, which contributes to disease. To investigate...
The ability of metabolically active tissues to increase glucose uptake in response insulin is critical whole-body homeostasis. This report describes the Dual Tracer Test, a robust method involving sequential retro-orbital injection [14C]2-deoxyglucose ([14C]2DG) alone, followed 40 min later by [3H]2DG with maximal dose quantify both basal and insulin-stimulated 2DG same mouse. collection measures from single animal imperative for generating high-quality data since differences action may be...
Metabolic disease is caused by a combination of genetic and environmental factors, yet few studies have examined how these factors influence signal transduction, key mediator metabolism. Using mass spectrometry-based phosphoproteomics, we quantified 23,126 phosphosites in skeletal muscle five genetically distinct mouse strains two dietary environments, with without acute vivo insulin stimulation. Almost half the insulin-regulated phosphoproteome was modified background on an ordinary diet,...
High fructose diets are associated with an increased risk of liver cancer. Previous studies in mice suggest lipogenesis is a key mechanism linking high to tumour growth. However, these administered at supraphysiological levels. The aim this study was determine whether growth and were altered fed physiological To test this, we injected male C57BL/6 the carcinogen diethylnitrosamine then them without or ranging from 10 20 % total calories. Results showed ≥15 had significantly numbers...
A hallmark of cancer cells is their ability to reprogram nutrient metabolism. Thus, disruption this phenotype a potential avenue for anti-cancer therapy. Herein we used phenotypic chemical library screening approach identify molecules that disrupted metabolism (by increasing cellular oxygen consumption rate) and were toxic cells. From screen discovered 1,4-Naphthoquinone (referred as BH10) broad range cell types. BH10 has improved cancer-selective toxicity compared doxorubicin, 17-AAG,...
Abstract Hepatic glucose production (HGP) is required to maintain normoglycemia during fasting. Glucagon the primary hormone responsible for increasing HGP; however, there are many additional and metabolic factors that influence glucagon sensitivity. In this study we report bioactive lipid lysophosphatidic acid (LPA) regulates hepatocyte by antagonizing glucagon-induced expression of gluconeogenic enzyme phosphoenolpyruvate carboxykinase (PEPCK). Treatment hepatocytes with exogenous LPA...
Abstract Genetic and environmental factors play a major role in metabolic health. However, they do not act isolation, as change an factor such diet may exert different effects based on individual’s genotype. Here, we sought to understand how gene–diet interactions influenced nutrient storage utilization, determinant of disease. We subjected 178 inbred strains from the Drosophila genetic reference panel (DGRP) diets varying sugar, fat, protein. assessed starvation resistance, holistic...
Insulin resistance is a pathophysiological state defined by impaired responses to insulin and risk factor for several metabolic diseases, most notably type 2 diabetes. occurs in target tissues including liver, adipose skeletal muscle. Methods such as tolerance tests hyperinsulinaemic-euglycaemic clamps permit assessment of specific allow the study progression causes resistance. Here we detail protocol assessing action muscle anesthetized mice administered with intravenously.
Research into cellular metabolism has become more high-throughput, with typical cell-culture experiments being performed in multiwell plates (microplates). This format presents a challenge when trying to collect gaseous products, such as carbon dioxide (CO2), which requires sealed environment and vessel separate from the biological sample. To address this limitation, we developed gas trapping protocol using perforated plastic lids plates. We used trap design measure CO2 production glucose...
Abstract Metabolic disease is caused by a combination of genetic and environmental factors, yet few studies have examined how these factors influence signal transduction, key mediator metabolism. Using mass spectrometry-based phosphoproteomics, we quantified 23,126 phosphosites in skeletal muscle five genetically distinct mouse strains two dietary environments, with without acute vivo insulin stimulation. Almost half the insulin-regulated phosphoproteome was modified background on an...
Metabolic disease is caused by a combination of genetic and environmental factors, yet few studies have examined how these factors influence signal transduction, key mediator metabolism. Using mass spectrometry-based phosphoproteomics, we quantified 23,126 phosphosites in skeletal muscle five genetically distinct mouse strains two dietary environments, with without acute vivo insulin stimulation. Almost half the insulin-regulated phosphoproteome was modified background on an ordinary diet,...
Despite the fact that genes and environment are known to play a central role in islet function, our knowledge of how these parameters interact modulate insulin secretory function remains relatively poor. Presently, we performed ex vivo glucose-stimulated secretion content assays islets 213 mice from 13 inbred mouse strains on chow, Western diet (WD), high-fat, carbohydrate-free (KETO) diet. Strikingly, among strains, commonly used C57BL/6J strain were least glucose responsive. Using matched...
Abstract Disclosure: L.M. Velez: None. C. Johnson: I. Tamburrini: M. Zhou: Viesi: N. Ujagar: D. Ashbrook: Nelson: A. Senior: James: R. Williams: Nicholas: Seldin: Polycystic ovary syndrome (PCOS) is the most common endocrinopathy in women, with a prevalence of ∼4-20% women reproductive age. The diagnosis generally occurs when patient consults for fertility issues and only based on criterion, which includes (1) hyperandrogenism, (2) oligo-anovulation, (3) polycystic morphology. However,...
Abstract Disclosure: L.M. Velez: None. C. Johnson: I. Yoon: K. Aberra: R. Feng: Tamburini: N. Ujagar: M. Nelson: A. Senior: D. Ashbrook: Williams: Nicholas: Seldin: Polycystic ovary syndrome (PCOS) is one of the most common female endocrinopathies, and frequently associated with major metabolic abnormalities such as insulin resistance, cardiovascular disease, obesity. Despite prevalence PCOS, genetic architecture subsequent interactions reproductive variables remain relatively unexplored....