A5072884918- Advanced Proteomics Techniques and Applications
- Radiation Therapy and Dosimetry
- Metabolomics and Mass Spectrometry Studies
- Effects of Radiation Exposure
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
- Advanced NMR Techniques and Applications
- Protein Structure and Dynamics
- Kruppel-like factors research
- Fibroblast Growth Factor Research
- Radiation Effects and Dosimetry
- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- Cell Image Analysis Techniques
- Advances in Oncology and Radiotherapy
- Advanced Radiotherapy Techniques
- Epigenetics and DNA Methylation
- Metabolism, Diabetes, and Cancer
The University of Sydney
2012-2019
Technical University of Munich
2012-2013
FGF21 improves the metabolic profile of obese animals through its actions on adipocytes. To elucidate signaling network responsible for mediating these effects, we quantified dynamic changes in adipocyte phosphoproteome following acute exposure to FGF21. regulated a 821 phosphosites 542 proteins. A major FGF21-regulated node was mTORC1/S6K. In contrast insulin, activated mTORC1 via MAPK rather than canonical PI3K/AKT pathway. Activation mTORC1/S6K by surprising because this is thought...
Insulin triggers an extensive signaling cascade to coordinate adipocyte glucose metabolism. It is considered that the major role of insulin provide anabolic substrates by activating GLUT4-dependent uptake. However, stimulates phosphorylation many metabolic proteins. To examine implications this on metabolism, we performed dynamic tracer metabolomics in cultured adipocytes treated with insulin. Temporal analysis metabolite concentrations and labeling revealed rapid distinct changes favoring...
This study investigates a model cell as target for low-dose radiation using Monte Carlo simulations. Mono-energetic electrons and photons are used with initial energies between 10 50 keV, relevant to out-of-field radiotherapy scenarios where modern treatment modalities expose relatively large amounts of healthy tissue radiation, also microbeam irradiation studies which show the importance cytoplasm target. The relative proportions number ionizations total energy deposit in nucleus...
The long-held view that radiation-induced biological damage must be initiated in the cell nucleus, either on or near DNA itself, is being confronted by mounting evidence to suggest otherwise. While efficacy of death may determined radiation nuclear DNA, a plethora less deterministic responses has been observed when not targeted. These so-called nontargeted cannot understood framework DNA-centric radiobiological models; what needed are new physically motivated models address damage-sensing...
In response to stimuli, biological processes are tightly controlled by dynamic cellular signaling mechanisms. Reversible protein phosphorylation occurs on rapid time-scales (milliseconds seconds), making it an ideal carrier of these signals. Advances in mass spectrometry-based proteomics have led the identification many tens thousands sites, yet for majority kinase is unknown and underlying network topology networks therefore remains obscured. Identifying substrate relationships (KSRs)...
We performed in silico microbeam cell irradiation modelling to quantitatively investigate ionisations resulting from soft x-ray and alpha particle microbeams targeting the cytoplasm of a realistic model. Our results on spatial distribution show that as x-rays are susceptible scatter within can lead nucleus, may not be suitable for investigating DNA damage response radiation alone. In contrast, an ideal tightly confined cytoplasm, but degrades upon interaction with components upstream...
Table of contents A1 Highlights from the eleventh ISCB Student Council Symposium 2015 Katie Wilkins, Mehedi Hassan, Margherita Francescatto, Jakob Jespersen, R. Gonzalo Parra, Bart Cuypers, Dan DeBlasio, Alexander Junge, Anupama Jigisha, Farzana Rahman O1 Prioritizing a drug’s targets using both gene expression and structural similarity Griet Laenen, Sander Willems, Lieven Thorrez, Yves Moreau O2 Organism specific protein-RNA recognition: A computational analysis complex structures different...