A5012845682- Monoclonal and Polyclonal Antibodies Research
- Kruppel-like factors research
- Glycosylation and Glycoproteins Research
- Advanced Fluorescence Microscopy Techniques
- Wnt/β-catenin signaling in development and cancer
- bioluminescence and chemiluminescence research
- Immune Response and Inflammation
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- HER2/EGFR in Cancer Research
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Prostate Cancer Treatment and Research
- Hedgehog Signaling Pathway Studies
- Cancer-related gene regulation
- Immune cells in cancer
- Genetic Syndromes and Imprinting
- Platelet Disorders and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Advanced Biosensing Techniques and Applications
- Protein Tyrosine Phosphatases
- Regulation of Appetite and Obesity
- Adipokines, Inflammation, and Metabolic Diseases
Academic Center for Education, Culture and Research
2010-2023
Tehran University of Medical Sciences
2017-2020
The receptor tyrosine kinase (RTK) ROR1 is overexpressed and of importance for the survival various malignancies, including lung adenocarcinoma, breast cancer chronic lymphocytic leukemia (CLL). There limited information however on in melanoma. In present study we analysed seven melanoma cell lines expression phosphorylation as well effects anti-ROR1 monoclonal antibodies (mAbs) suppressing siRNA survival. was at protein level to a varying degree phosphorylated serine residues. Three our...
Background Melanoma has increased in incidence worldwide prompting investigators to search for new biomarkers targeted immunotherapy of this disease. Placenta specific 1 (PLAC1) is a member cancer-testis antigens with widespread expression many types cancer. Here, we aimed study the first time pattern PLAC1 skin cancer samples including cutaneous melanoma, basal cell carcinoma (BCC), squamous (SCC) comparison normal and nevus tissues potential therapeutic effect anti-PLAC1 antibody melanoma...
Abstract Our recent findings strongly support the idea of PLAC1 being as a potential immunotherapeutic target in prostate cancer (PCa). Here, we have generated and evaluated an anti-placenta-specific1 (PLAC1)-based antibody drug conjugate (ADC) for targeted immunotherapy PCa. Prostate cells express considerable levels PLAC1. The Anti-PLAC1 clone, 2H12C12, showed high reactivity with recombinant selectivity recognized but not LS180 cells, negative control. binding induced rapid...
Citation Jeddi‐Tehrani M, Torabi R, Mohammadzadeh A, Arefi S, Keramatipour Zeraati H, Zarnani AH, Akhondi MM, Mahmoudian J, Mahmoudi AR, Zarei S. Investigating association of three polymorphisms coagulation factor XIII and recurrent pregnancy loss. Am J Reprod Immunol 2010; 64: 212–217 Problem Among important suspected causes thrombophilia in women with loss (RPL) are the (FXIII) gene. We performed a case–control study on between (FXIII G103T, FXIII A614T C1694T) RPL Iranian women. Method...
HER2 proto-oncogene is critical in the biology of breast cancer and an important therapeutic target monoclonal antibodies (mAbs). We have recently established a panel anti-HER2 mAbs recognizing different epitopes within extracellular domain HER2.In present study antiproliferative effect these was investigated on HER2-overexpressing human cell line BT474, using radioactive thymidine incorporation assay.Our results demonstrated that while two (1T0 2A8) inhibited proliferation dose dependently,...
Leptin is an important protein that regulates energy storage and homeostasis in humans animals. deficiency results various abnormalities such as diabetes, obesity, infertility. Producing a high affinity monoclonal antibody against human leptin provides tool to monitor trace function different biological fluids. In this study, recombinant was conjugated KLH injected into mice. After immunization, mouse myeloma SP2/0 cells were fused with murine splenocytes followed by selection of...
ABSTRACT Nowadays, fluorescent dyes have become significant tools in immunostaining experiments. Different various physicochemical traits. Some of these traits, for instance photobleaching and photostability, are applicable calibers such experiments as immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry (FC). In this study, photobleaching, mean fluorescence intensity (MFI) FITC (fluorescein isothiocyanate)- Dylight488- conjugated Herceptin, the humanized anti Her2...
Placenta-specific 1 (PLAC1) is among recently-discovered placental antigens which exerts fundamental role in function and development. Increasing body of literature shows that PLAC1 frequently activated expressed a wide variety human cancers promote cancer progression. However, no data available regarding the expression mouse orthologue, plac1, murine cell lines. Materials Methods: We investigated plac1 series lines from different histological origins, mammary carcinoma (4T1), melanoma...
Opticin (OPTC) is a member of the small leucine-rich proteoglycan (SLRP) family and localized particularly in certain extracellular matrices. We have previously reported unique expression another SLRP, fibromodulin (FMOD) leukemic cells patients with chronic lymphocytic leukemia (CLL). OPTC located same region as FMOD on chromosome 1 (1q32.1). Cluster up-regulation genes may be observed malignancies aim present study was to analyze CLL cells.The tested by RT-PCR realtime qPCR PBMC from...
Factor VII (FVII) is a serine protease-coagulating element responsible for the initiation of an extrinsic pathway clot formation. Here we generated and characterized high affinity monoclonal antibody that specifically recognizes human FVII. Recombinant FVII (rh-FVII) was used production using BALB/c mice. The specificity determined by Western blot plasma samples from human, mouse, sheep, goat, bovine, rabbit, rat. Furthermore, to detect transiently expressed rh-FVII in BHK21 cell line...
Actin is one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells with important roles many cell functions. Antibodies against β-actin other gene-encoded are used as internal loading controls Western blot analyses. The aim this study was to produce a monoclonal antibody (mAb) synthetic peptide derived from N-terminal region its reactivity different organisms. A peptide, β-actin, designed mAb by hybridoma technology. produced (clone 4E5- A10)...
Fluorescent dyes are excited by light and emit at a longer wavelength. Photobleaching is one the most important obstacles in fluorescent image capturing. Photochemical alteration of dye caused several excitation/emission cycles results fluorophore to be unable light. In this study, R-phycoerythrin (R-PE) Alexa Fluor 568 were separately conjugated streptavidin. The efficiency conjugations, R-PE-streptavidin streptavidin-Alexa 568, evaluated sodium dodecyl sulfate polyacrylamide gel...
Abstract Background Placenta-specific 1 (PLAC1) is one of the oncoplacental genes ectopically expressed in a wide variety cancers. Antibody drug conjugates (ADC) have potential to substantially improve efficacy and reduce toxicity treatment compared with cytotoxic small-molecule drugs are recently being employed for Here, SN38-conjugated monoclonal anti-PLAC1 antibody was examined breast cancer. Methods Results Anti-human PLAC1 antibodies were produced characterized. SN38 conjugated an...