A5113604804- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Galectins and Cancer Biology
- Immunotherapy and Immune Responses
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Blood groups and transfusion
- Advanced biosensing and bioanalysis techniques
- Chronic Myeloid Leukemia Treatments
- HER2/EGFR in Cancer Research
- Reproductive System and Pregnancy
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- SARS-CoV-2 detection and testing
- Wnt/β-catenin signaling in development and cancer
- Electrospun Nanofibers in Biomedical Applications
- Platelet Disorders and Treatments
National Institute of Genetic Engineering and Biotechnology
2023-2025
Shahid Beheshti University
2023-2025
Academic Center for Education, Culture and Research
2014-2025
Karolinska University Hospital
2002-2012
Karolinska Institutet
1997-2011
Pasteur Institute of Iran
2005
Tehran University of Medical Sciences
2005
Jan Kochanowski University
2005
University of Łódź
2005
Gene Therapy Laboratory
2000-2004
Abstract The aim of our study was to investigate the physical state and viral load HPV‐16 in tonsillar cancer correlate these findings with clinical outcome. To distinguish between integrated episomal forms HPV, 22 fresh‐frozen samples were analysed by a method based on restriction enzyme cleavage, ligation PCR ( rli PCR). detected 11/22 HPV‐33 1/22 cancers, hence 12/22 (55%) tumours HPV positive. Only extrachromosomal observed. Full‐length exclusively 7/11 whereas both full‐length deleted...
Abstract Gene profiling studies of patients with chronic lymphocytic leukemia (CLL) has revealed increased expression Ror1, a cell surface receptor tyrosine kinase. The aim present study was to analyze gene and protein Ror1 in CLL cells normal blood leukocytes. analysis reverse transcription‐polymerase chain reaction ROR1 that all ( n = 100) spontaneously expressed mRNA whereas enriched B T as well granulocytes from healthy donors 10) were negative. A strong nonphysiological activation...
IFN-γ regulates the immunogenicity of target cells by increasing their expression HLA class I molecules. This facilitates T cell receptor–mediated recognition CD8+ but decreases sensitivity to lysis NK due engagement inhibitory receptors. In this study, short-term tumor lines from patients with advanced ovarian carcinomas were established. We demonstrate paradoxical finding that treatment these carcinoma (OVACs) resulted in resistance peptide- and allospecific cells. Blocking experiments...
IFN-γ regulates the immunogenicity of target cells by increasing their expression HLA class I molecules. This facilitates T cell receptor–mediated recognition CD8+ but decreases sensitivity to lysis NK due engagement inhibitory receptors. In this study, short-term tumor lines from patients with advanced ovarian carcinomas were established. We demonstrate paradoxical finding that treatment these carcinoma (OVACs) resulted in resistance peptide- and allospecific cells. Blocking experiments...
The receptor tyrosine kinase ROR1 has been shown to be overexpressed in chronic lymphocytic leukemia (CLL). aim of this study was further characterize the expression and other member ROR family, ROR2, lymphoid myeloid malignancies. Normal white blood cells reactive lymph nodes were negative for ROR2. A significantly high uniform surface found CLL/hairy cell (HCL) compared mantle lymphoma (MCL), marginal zone (MZL), diffuse large B-cell (DLBCL), follicular (FL), myelomas, acute lymphoblastic...
Herein, a novel heterogeneous nanoscale reducing agent for antibody cleavage, made of iron oxide nanoparticles, silica network, palladium on calcium carbonate (10%), and dithiothreitol (Fe3O4@Pd/CaCO3-DTT), is presented as substantial alternative traditional homogeneous analogues. Conventionally, fragmentation accomplished using agents proteases that digest or cleave certain portions the immunoglobulin protein structure to provide active thiol sites drug tagging aims. Then, dialysis process...
The unstoppable spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has severely threatened public health over the past years. current ubiquitously accepted method for its diagnosis provides sensitive detection virus; however, it is relatively time-consuming and costly, not to mention need highly skilled personnel. There a clear develop novel computer-based diagnostic tools provide rapid, cost-efficient, time-saving in places where massive traditional testing practical....
Abstract IgD has been suggested to be a recently developed Ig class, only present in rodents and primates. However, this paper the cow, sheep, pig δ genes have identified shown transcriptionally active. The deduced amino acid sequences from their cDNAs show that artiodactyl H chains are structurally similar human IgD, where chain constant regions all contain three domains hinge region, sharing homologies of 43.6, 44, 46.8% with counterpart, respectively. According phylogenetic analysis, Cδ...
To estimate the prevalence of chronic granulomatous disease (CGD) in Sweden, an inquiry asking for known and possible CGD cases was mailed to paediatric, internal medicine infectious departments all over Sweden. The detected patients were characterized as genetics clinical presentation. Twenty–one (belonging 16 different families) found, corresponding a ∼ 1/450 000 individuals. with X–linked disease, lacking functional gp91 phox protein ( n = 12), comprised 57% 43% had autosomal recessive...
To investigate why human leukocyte-associated antigen-DRB1*0301 (HLA-DRB1*0301) positive Scandinavian patients have a better prognosis than HLA-DRB1*0301 negative patients, the present authors examined patterns of cytokine expression in bronchoalveolar lavage (BAL) cells and BAL fluid (BALF) from with pulmonary sarcoidosis controls. Using real-time PCR, mRNA selected cytokines newly diagnosed, untreated nonsmoking (n = 25) controls 11) was quantified. Cytokine protein levels BALF 34) were...
Abstract Purpose and Experimental Design: Twenty-eight patients with immunoglobulin G myeloma stages I to II were immunized i.d. over 110 weeks autologous M protein combined interleukin-12 (IL-12; n = 15) or IL-12 granulocyte macrophage colony-stimulating factor (GM-CSF; 13). Idiotype-specific T-cell responses assessed by [3H]thymidine incorporation, enzyme-linked immunospot assay, delayed-type hypersensitivity reaction. Results: Based on these three assays, idiotype-specific immune noted in...
Cernunnos is involved in the nonhomologous end-joining (NHEJ) process during DNA double-strand break (DSB) repair. Here, we studied immunoglobulin (Ig) class switch recombination (CSR), a physiological which relies on proper repair of DSBs, B cells from Cernunnos-deficient patients. The pattern vivo generated CSR junctions altered these cells, with unusually long microhomologies and lack direct end-joining. patients largely resemble those lacking ligase IV, Artemis, or ATM, suggesting that...
Summary Chicken antibodies are increasingly being used as diagnostic and therapeutic tools. As only the genomic organization of µ encoding gene was previously known, we analysed chicken immunoglobulin Y (IgY) IgA (υ α chain) heavy‐chain constant region (IGHC) genes IGHC locus. The is encoded by four separate exons, whereas, surprisingly, there no intervening DNA sequence between CH1 CH2 domains IgY heavy chain, which thus three exons separated two introns. analysis shows that exon boundaries...
Summary T‐cell immune dysfunction in patients with malignant tumours has been attributed to the altered expression of components receptor (TCR)/CD3 complex and their associated intracellular protein tyrosine kinases. In this study, four‐colour flow cytometry was applied study surface bound molecules TCR α β , CD28, CD152 CD154 involved signalling signal transduction CD3 ζ p56 lck p59 fyn ZAP‐70 phosphatidyl‐inositol‐3 kinase (PI3‐k) as well cytokines interferon‐ γ (IFN‐ ), interleukin (IL)‐4...
We present a method for conjugating antigens to gold nanoparticles (GNPs) during their synthesis via gas plasma, eliminating the need chemical linkers and significantly speeding up process (taking only 15 min). This fast, linker-free produces biocompatible stable GNPs, with potential immunotherapy applications, such as antigen antibody conjugation drug delivery. demonstrate of Nestin (NES), tumor marker, GNPs using two approaches. The first involves synthesizing citrate, followed by NES...